Báo cáo khoa học: The propagation of hamster-adapted scrapie

Báo cáo khoa học: The propagation of hamster-adapted scrapie PrPSc can be enhanced by reduced pyridine nucleotide in vitro pdf

... Calculat- ing the signal intensity of the reactions of 0 and 10 lm NADPH revealed that the efﬁciency of PMCA was increased by  2.59-fold in the presence of NADPH. To evaluate whether the effect of ... series of experiments was conducted to investigate the possible in uences of pyridine nucleotides on the propagation activities of hamster-adapted scra...

Ngày tải lên : 07/03/2014, 03:20

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Báo cáo khoa học: The properties of phosphodiesterase 11A4 GAF domains are regulated by modiﬁcations in its N-terminal domain pptx

... regulating the cGMP afﬁnity of the PDE11A4 tandem GAF domain and thus allosterically affect PDE11 activity. The role of the N-terminus of the PDE11A4 GAF tandem domain The N-termini that precede the ... from apparent inhibition by its N-terminus and increased the cGMP afﬁnity of the GAF tandem domain up to 20-fold (Figs 1 and 2B). Maximal stimulation by cGMP was...

Ngày tải lên : 16/03/2014, 06:20

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Báo cáo khoa học: Selective detection of superoxide anion radicals generated from macrophages by using a novel ﬂuorescent probe pdf

... reaction system increased with increasing time up to 10 min and became constant thereafter. This shows that the probe can capture O 2 – Æ quickly within 10 min, which attests to DBZTC being a ‘fast ... concentration The pH of the medium has a large effect on ﬂuores- cence intensity. Hepes was used because it has been shown to be a better buffer for the incubation of mammalian...

Ngày tải lên : 16/03/2014, 11:20

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Tài liệu Báo cáo khoa học: The occurrence of hemocyanin in Hexapoda docx

... by three histidines in two distinct binding sites. Some hemocyanins assemble into large oligomers of up to 8 · 6 subunits [1]. The occurrence and properties of hemocyanins have been thoroughly ... respiratory proteins have long been considered unnecessary [10– 12]. Nevertheless, a functional hemocyanin has been identiﬁed in the hemolymph of the stoneﬂy Perla mar- ginata [8]....

Ngày tải lên : 18/02/2014, 08:20

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Tài liệu Báo cáo khoa học: Mitochondrial targeting of intact CYP2B1 and CYP2E1 and N-terminal truncated CYP1A1 proteins in Saccharomyces cerevisiae ) role of protein kinase A in the mitochondrial targeting of CYP2E1 pdf

... of PKA in the mitochondrial targeting of CYP2E1 The role of PKA in mitochondrial targeting of CYP2E1 was investigated using two approaches. The ﬁrst approach involved measuring the level of mitochondrial ... were initiated by the addition of 3 mm NADPH, and incubation was continued for 30 min at 37 °C in a shaking water bath. The reactions were terminated by...

Ngày tải lên : 18/02/2014, 16:20

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Tài liệu Báo cáo khoa học: The effect of small molecules in modulating the chaperone activity of aB-crystallin against ordered and disordered protein aggregation pdf

... a-lactalbumin was maintained in the presence of intermediate concentrations of Arg-HCl, but was not further enhanced by it (Fig. 1C). The sig- niﬁcant decrease in the amount of precipitation in the ... interact with a number of functional groups, including the aromatic side chains of some amino acids, through a stacking mechanism [42]. The interaction of argi...

Ngày tải lên : 18/02/2014, 16:20

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Tài liệu Báo cáo khoa học: The sequentiallity of nucleosomes in the 30 nm chromatin ﬁbre pptx

... and can be assessed quantitatively) in the dinucleosome sample is indistinguishable from that of high-molecular weight chromatin. Furthermore, in the DNase I foot- print of the dinucleosome, the ... of this material with trypsin initially caused a gradual decrease of the number of nucleosomes per chain in the main peak, with a corresponding increase in the...

Ngày tải lên : 18/02/2014, 18:20

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Tài liệu Báo cáo khoa học: The stereochemistry of benzo[a]pyrene-2¢-deoxyguanosine adducts affects DNA methylation by SssI and HhaI DNA methyltransferases pptx

... large increase in ﬂuorescence in the case of the complex of M.SssI with the G CY + ⁄ CGM duplex containing the (+)-trans adduct in the CpG site may be caused by diminished quenching by the target ... 5B). The DNA molecule is located in the cleft formed between the two domains with the major groove facing the small domain and the minor groove facing t...

Ngày tải lên : 19/02/2014, 00:20

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Tài liệu Báo cáo khoa học: The localization of FGFR3 mutations causing thanatophoric dysplasia type I differentially affects phosphorylation, processing and ubiquitylation of the receptor pptx

... phosphorylation of proteins involved in the trafﬁcking of the receptor, including c-Cbl, could account for its intracellular retention. By contrast, TDI mutations in the ECD or disruption of the termination ... However, the possible involvement of the adaptor proteins FRS2 and Grb2 in the ubiquitinylation process cannot be excluded [31,40]. Alternatively, other...

Ngày tải lên : 19/02/2014, 00:20

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Tài liệu Báo cáo khoa học: The role of electrostatic interactions in the antitumor activity of dimeric RNases docx

... through the exchange between subunits of either their N-terminal, or their C-terminal ends [33–35]. We found that the behavior with the model membrane of the dimer in which the C-terminal ends ... contribution of the protein to the degree of polarization of the ﬂuorescence probe. Leakage of vesicle aqueous contents induced by the RNases was measured by t...

Ngày tải lên : 19/02/2014, 06:20

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