Báo cáo khoa học: Studies on the role of the receptor protein motifs possibly involved in electrostatic interactions on the dopamine D1 and D2 receptor oligomerization pdf

... dimerization FEBS Journal 276 (2009) 760–775 ª 2008 The Authors Journal compilation ª 2008 FEBS 775 Studies on the role of the receptor protein motifs possibly involved in electrostatic interactions on ... USA) and Sigma (Poznan ´ , Poland). Construction of fusion proteins The human dopamine D 1 and D 2 receptor genes were cloned into the pcDNA3.1...

Ngày tải lên: 07/03/2014, 03:20

... crystal- lization conditions included an ammonium ion concen- tration of 1.9 m, which probably in uences the nature of the cation under crystallization conditions. The function of the ion is presumably ... results of the decarboxylation assays of the ‘bulky’ mutants all point to the orientation of H333 and its hydrogen bond donor properties as being the cornerst...

Ngày tải lên: 19/02/2014, 08:20

... monomer (Table 1). The sub-stoichiometric iron content of iron–sulfur proteins is generally due to the impaired incorporation of the cluster into the protein during overexpression in E. coli and ... in gene expression after the deletion of whiB7 [6] could be seen as the absence of a component which possibly exerts its function via a protein protein interaction...

Ngày tải lên: 18/02/2014, 12:20

... function of OsDmc1 protein concentration (Fig. 4A, lines 2 and 3). How- ever, at concentrations higher than 2.5 lm OsDmc1, there was only an increase in the rate, but no further increase in the ... function of reac- tion time, at a fixed concentration of protein, by varying nucleotide cofactor conditions. The reaction was the fastest when it contained ATP, where within a...

Ngày tải lên: 19/02/2014, 07:20

... was found to be increased upon overexpression of the deubiquitinating enzyme otubain 1 (OTUB1), a member of the ovarian tumour domain-containing protein family. Conversely, OTUB1 knockdown interfered ... the intestine. The Yersinia surface protein invasin binds to b1 integrins on the apical surface of M cells, which facilitates translocation across the epithelium [4,5]...

Ngày tải lên: 16/02/2014, 15:20

... in Swiss-Prot and similar databases. Structure and function of the linker histone variants Location and structure of the linker histones Historically, the location of the globular domain of the linker ... modifications at Lys26, and at posi- tion 27 there is an alanine, rather than a serine [43]. Ubiquitination and formylation For the first time, ubiquitinations...

Ngày tải lên: 18/02/2014, 08:20

... content of the thrombi [4]. These lipid constituents of thrombi profoundly modu- late the fibrinolytic process [2,3,5–7]. In the few studies evaluating the effect of long-chain fatty acids on plasmin ... by plasmin (EC 3.4.21.7.), contain up to millimolar concentrations of fatty acids and these are known to affect the action of the protease. In the present stu...

Ngày tải lên: 18/02/2014, 17:20

... important roles in the induction of mitochondria-mediated apoptosis and the inhibition of invasion, along with the downregulation of matrix metalloproteinase expression. In addition, by using the calcium ... from the ER has been described as one of the mechanisms of this regulation. On the basis of the above findings, our research provides new insights into...

Ngày tải lên: 18/02/2014, 18:20

... PE-containing membranes, but rather for anionic membranes contain- ing PG [16], and little information is available regard- ing the phospholipid and membrane binding of the other members of the ... to date, and include the association with phospholipids and membranes [13–16], the inhibition of Raf1 kinase [17,18], thrombin [19], and G -protein- coupled receptor kin...

Ngày tải lên: 19/02/2014, 05:20

... titration curve having two in exion points, whose difference along the abscissa corresponded to the amount of acid required for the protonation of the amine groups of samples. The number of equivalents ... min (4 h) after which there was a decline (Fig. 4C,D), indicating enzyme stability in the pH range 2.0–5.0 and 45–60 °C in the initial hours. The effect of...

Ngày tải lên: 19/02/2014, 12:20