Báo cáo khoa học: Unusual stability of human neuroglobin at low pH – molecular mechanisms and biological significance docx

... conditions of acidic pH, is essentially a random coil polypeptide. Urea-mediated denaturation studies revealed that the stability displayed by apoNgb at pH 2.0 is very similar to that of Mb at pH 7.0. ... maximum at 287 nm and the minimum at 283 nm and that between the maximum at 287 nm and the mini- mum at 295 nm were used to calculate the Tyr exposure. Unusual...

Ngày tải lên: 07/03/2014, 02:20

... panel). At low pH, bk cat is larger than k cat , but at high pH bk cat is smaller than k cat .Thatistosay, A328W goes from substrate activation, at low pH, to substrate inhibition, at high pH. A ... bk cat and k cat at high pH is large relative to the difference at low pH, and the difference between bk cat at high pH and bk cat at low pH is relatively...

Ngày tải lên: 20/02/2014, 23:20

... Unfolding of triosephosphate isomerase from Trypanosoma brucei: identification of intermediates and insight into the denaturation pathway using tryptophan mutants. Arch. Biochem. Biophys. 399, 11 7–1 29. 51. ... with ammonium sulfate at a saturation of 7 0–9 0% followed by anion-exchange chromatography using a ResourceQ (6 mL; Pharmacia) column at pH 8.0 (20 m M Tris/HCl) wit...

Ngày tải lên: 31/03/2014, 07:20

... enzymes, membranes and molecules to cope with the heat. At low temperatures, the chemical reaction rates are lower, and hence, in order to be competitive and grow fast at low temperatures, evolu- tionary ... substrate, and it is possible that the enzymes also have adapted strategies to remove Na + around the phosphates of DNA before catalysis can take place. It seems clear t...

Ngày tải lên: 19/02/2014, 05:20

... plasmid for GST and GST-fused proteins (GST– IST1, GST–IST1MIM, GST–IST1MIM L326D,L353A , GST– CHMP6NT, GST–pHis and GST–MIT–pHis). Expression of GST–IST1, GST–IST1MIM and GST–IST1 L326D,L353A was ... were incu- bated with glutathione–Sepharose beads immobilizing GST–pHis (negative control) or GST–MIT–pHis for 2 h at 4 °C with gentle mixing. After Sepharose beads had been recovered...

Ngày tải lên: 18/02/2014, 04:20

... gel-filtration Fig. 2. Effect of orthophosphate and di- and tri-polyphosphates on oligomerization of native hTK1. hTK1 was diluted and incubated with 3 m M of the indicated nucleotides or phosphate ... absence of MgCl 2 , as the combination of MgCl 2 and Chaps causes a heavy precipitate. Fig. 1. Effect of nucleotides and polyphos- phates on oligomerization of native hTK...

Ngày tải lên: 18/02/2014, 12:20

... Isomers of 3-MG-CoA as substrates for human 3-MG-CoA hydratase (AUH). (A) 3-MG-CoA was enzymatically synthesized by incuba- ting 100 m M 3-methylglutaconate in 100 mM potassium phosphate pH 7.0 ... cleavage of citrate on inactivated citrate lyase. Eur J Biochem 37, 29 5–3 04. 26 Loupatty FJ, Ruiter JPLIJ, Duran M & Wanders RJ (2004) Direct nonisotopic assay of 3-methylglutacon...

Ngày tải lên: 19/02/2014, 07:20

... Eu 3+ at 320 nm and monitoring the XL665 emission at 665 nm. Analysis of data Data were analysed using the computer program GRAPHPAD PRISM (GraphPad Software Inc.). [ 3 H]Spiperone saturation binding ... monoclonal anti-gp120 Ig; NIBSC) for 1 h at 4 °C on a rotating wheel. Then, 2 5–5 0 lg of protein G–sepharose (Sigma) was added and incubation was carried out at 4 °C overni...

Ngày tải lên: 21/02/2014, 00:20

... of incubation (lane 2), the bands at 19 and 20 kDa had disappeared, and two clear bands appeared at 15.0 and 13.3 kDa. These bands are thought to be fragments of hTNF-a .At2 0hof incubation (lane ... 8 h of incu- bation, the bands at 19 and 29 kDa had disappeared, and two clear bands appeared at 15.0 and 13.3 kDa. These bands are thought to be fragments of hTNF-a....

Ngày tải lên: 06/03/2014, 22:21

... Chem 28, 1–2 . 42 Kabra PM, Lee HK, Lubich WP & Marton LJ (1986) Solid-phase extraction and determination of dansyl derivatives of unconjugated and acetylated polyamines by reversed-phase liquid ... of immunofluorescent staining of SMO in cells overex- pressing each of the isoforms provided direct visuali- zation and confirmation of protein localization without the possi...

Ngày tải lên: 07/03/2014, 05:20