... mediaStrains W303-1B (mat a, ade2 1, ura3 1, his3 11 , trp1 1, leu2–3 ,11 2, can1 10 0), FYF4-A1 aco1 (mat a, ura3–52,trp1D63, leu2D1, his3D200, aco1::URA3), and BY4 7 41 (mat a, his3, leu2, met15, ura3) ... stained.Lane 1, recombinant human IRP1; lanes 2– 11 , fractions 1 10 of the sucrose gradient.(B) Western blot of the same fractions.Lane 1, recombinant human IRP1; lanes 2– 11 , fractions 1 10 of the ... property of human IRP1. The active participation of mitochondrialKGD1p is borne out by the lack of aggregates in aKGD1 mutant (Fig. 6) and implies that association of IRP1 and KGD1p eludes...