Báo cáo khoa học: SAF-3, a novel splice variant of the SAF-1/MAZ/Pur-1 family, is expressed during inflammation pptx
Báo cáo khoa học: SAF-3, a novel splice variant of the SAF-1/MAZ/Pur-1 family, is expressed during inflammation pptx
... variant of the SAF-1/MAZ/Pur-1
family, is expressed during inflammation
Alpana Ray
1
, Srijita Dhar
1
, Arvind Shakya
1
, Papiya Ray
1
, Yasunori Okada
2
and Bimal K. Ray
1
1 Department of Veterinary ... CCATTCCAGgtgagtag 84 ctccgcagGCCGCGCCG
2 851 CTTCTCCCGgtgtgcac 403 gtccccagGCCGGATCA
364AATGTGAGgtaggaag 277 ctcctcagAAATGTGAG
4 172 CAACAAAGgtacatgc 1335 ctgtgcagGTACTGGTG...
Báo cáo khoa học: Phaiodotoxin, a novel structural class of insect-toxin isolated from the venom of the Mexican scorpion Anuroctonus phaiodactylus pdf
... tran-
scriptase. The cDNA was joined with the a daptor provided
by the kit (5 ¢-gcugauggcgaugaaugaacacugcguuugCUGG
CUUUGAUGAAA-3¢) using T4 DNA ligase. The modi-
fied cDNA was used as t emplate for PCR amplification.
Two ... scorpions of the family
Buthidae, probably because they are dangerous to humans.
A large number of different protein and polypeptides have
been isolated a...
Tài liệu Báo cáo khoa học: TransLISA, a novel quantitative, nonradioactive assay for transcription factor DNA-binding analyses pdf
... h
D
1 h
Autoradiography
Add D beads and incubate
D A
O
2
Read AlphaLISA si
g
nal at 615 nm
HSE
Fig. 1. Comparison of EMSA and TransLISA for the detection of HSF1–DNA binding activity. (A) Schematic ... between the addition of reagents.
The broad analytical range of the assay allows quanti-
tation of large differences in the DNA-binding activity
of transcription factors...
Tài liệu Báo cáo khóa học: TbPDE1, a novel class I phosphodiesterase of Trypanosoma brucei pdf
... 5¢-GGGAATTCCATATGAGAGACAATA
TTTCCCGTTTATCAAATC-3¢ and 5¢-CCGCTCGAGT
CATTACTAGGTTCCCTGTCCAGTGTTACC-3¢,and
PDE1(Lys321–Thr620) was amplified with primers 5¢-GGG
AATTCCATATGAAGAATGATCAATCTGGCTGCG
GCGCAC-3¢ and 5¢-CCGCTCGAGTCATTACTAGG
TTCCCTGTCCAGTGTTACC-3¢. ... explore the PDEs of parasites as potential
targets for antiparasitic drugs. The African trypanosome
Trypanosoma brucei is the p...
Báo cáo khoa học: Purification and structural analysis of the novel glycoprotein allergen Cyn d 24, a pathogenesis-related protein PR-1, from Bermuda grass pollen pot
... GGT AGC CAA 276
93QEGYGSTQLPSDEPLNGLND
CAA GAA GGA TAT GGC AGC ACC CAA CTT CCA TCG GAC GAG CCA TTG AAC GGG CTC AAC GAC 336
113KAIQDILNEHNMFRAKERVP
AAG GCC ATA CAG GAC ATC CTC AAC GAG CAC AAC ATG ... 156
53QEAVEGAEQVASESASLTTP
CAG GAG GCG GTA GAG GGC GCC GAG CAG GTA GCG TCC GAG TCA GCC TCC CTC ACA ACA CCA 216
73TTREEQPAAEAAASTAGGSQ
ACC ACC AGG GAA GAA CAA CCG GCG GCA GAG GCC GCG GCG TCC ACC GC...
Báo cáo khoa học: Brox, a novel farnesylated Bro1 domain-containing protein that associates with charged multivesicular body protein 4 (CHMP4) potx
... has a point
mutation at amino acid 408, was c reated by PCR-based
site-directed mutagenesis using pmGFP–Brox as a
template and complementary primers (5¢-CAA AAG
GAC ACT GGG TCC TAC ATC TCC TAA ... 52 789 4088
E-mail: mmaki@agr.nagoya-u.ac.jp
Database
The nucleotide sequence of the human Brox
cDNA is available in the DDBJ ⁄ EMBL ⁄ Gen-
Bank database under accession number
AB27612...
Báo cáo khoa học: Molecular cloning and functional expression of the human sodium channel b1B subunit, a novel splicing variant of the b1 subunit potx
... the
same splicing variant from human tissue. Here, we report
the cloning and characterization of a novel, splicing
variant of the human b
1
subunit by rapid amplification
of cDNA end polymerase chain ... shown are the mean (j) and standard deviation (bars) for each cRNA a: b ratio, and the sample size per
cRNA combination is shown in parenthesis. Data are from five diff...
Báo cáo khoa học: Plasmoredoxin, a novel redox-active protein unique for malarial parasites doc
... antioxidant protein
that is also involved in DNA synthesis may represent a
potential drug target.
Acknowledgements
The technical assistance of Elisabeth Fischer, Petra Harwaldt and Beate
Hecker is acknowledged. ... the His-tag
and 22 kDa) appeared when probing the recombinant
protein and a trophozoite extract of P. falciparum.This
result, and the fact that PfPlrx was ampli...
Báo cáo khoa học: "Towards a Semantic Classification of Spanish Verbs Based on Subcategorisation Information" doc
... are the 3-way and 15-way classifica-
tions. The baseline is calculated, for each task, as
the average value of the Adjusted Rand measure for
100 random cluster assignations. Although all the
tasks ... in
evaluating unsupervised classification tasks against
a gold standard lies in the fact that the class labels
of the obtained clusters are unknown. Therefore, the
evalua...
Báo cáo khoa học: Broad antibiotic resistance profile of the subclass B3 metallo-b-lactamase GOB-1, a di-zinc enzyme potx
... reverse:
(5¢-GATCTTGCTGCTTACT
GCGGCTCACTACGACC
ATACAGG-3¢)
(5¢-GCACCTGTATGGTCGTAGTGAGC
CGCAGTAAG
CAGC-3¢)
For the Q116N mutant forward and reverse:
(5¢-GATCTTGCTGCTTACT
AACGCTCACTACGACC
ATACAGG-3¢)
(5¢-GCACCTGTATGGTCGTAGTGAGC
GTTAGTAAG
CAGC-3¢)
For ... reverse:
(5¢-GATCTTGCTGCTTACT
AACGCTCACTACGACC
ATACAGG-3¢)
(5¢-GCACCTGTATGGTCGTAGTGAGC
GTTAGTAAG
CAGC-3¢)
For the Q116H mutant forward and...