Báo cáo khoa học: Phosphorylation-dependent binding of human transcription factor MOK2 to lamin A⁄C pot

... 3137–3147 ª 2009 The Authors Journal compilation ª 2009 FEBS 3147 Phosphorylation-dependent binding of human transcription factor MOK2 to lamin A ⁄ C Maryannick Harper, Jeanne Tillit, Michel Kress and ... A-type lamins have been shown to bind hsMOK2 in vitro and in vivo through the coil 2 domain common to lamin A and lamin C, whereas the lamin A ⁄ C -binding site...

Ngày tải lên: 07/03/2014, 01:20

... 2.2, at the end of each binding cycle. To measure the binding properties of Erbicin and ERB- hRNase to the extracellular domain of ErbB2, ECD was immobilized onto the surface of sensor chip CM5 ... Yamamoto T, Ikawa S, Akiyama T, Semba K, Nomura N, Miyajima N, Saito T & Toyoshima K (1986) Similar- ity of protein encoded by the human c-erb-B-2 gene to epidermal growth...

Ngày tải lên: 23/03/2014, 06:20

... the binding of Glc to the super-open state (Fig. 1C) of this two domain [large (L) and small (S)] enzyme, and thus the site of initia- tion of the global conformational transition; and (c) to gain ... structures of the two conformational states demonstrate large changes in the main-chain torsion angles of both regions on Glc binding. CR II, which Activation of glucokinase...

Ngày tải lên: 30/03/2014, 04:20

... penta-EF-hand domains [4], which contribute to activation of the pro- tease by Ca 2+ binding, to heterodimerization of each subunit, and to binding of the endogenous calpain inhibitor calpastatin [5,6]. Although ... be attributable to the presence of two potential binding sites in the IST1 C-terminal region, which contains Fig. 8. Autolytic properties of mGFP–calpain 7–...

Ngày tải lên: 18/02/2014, 04:20

... crystal structure of TK1-type enzymes of human and myco- plasmic origin [14], the feedback inhibitor dTTP was bound in the substrate pocket, similar to the binding of dTTP to the D. melanogaster ... B (2004) Effect of valine 106 on structure–function rela- tion of cytosolic human thymidine kinase. Kinetic prop- erties and oligomerization pattern of nine substitution muta...

Ngày tải lên: 18/02/2014, 12:20

... Biochem 244, 890–899. 18 Kurimoto K, Fukai S, Nureki O, Muto Y & Yokoyama S (2001) Crystal structure of human AUH protein, a single-stranded RNA binding homolog of enoyl-CoA hydratase. Structure ... group of the CoA-donor (R 1 -COO – ) to the carboxyl group of the CoA-acceptor (R 2 -COO – ). In the case of glutaconate CoA-transferase from A. fermentans, CoAS – transiently...

Ngày tải lên: 19/02/2014, 07:20

... concerted proton transfer to the leaving group of the substrate occurs. Proton transfer The requirement of an acceptor for the Oc proton from the catalytic serine is a critical component of the mechanism ... difference in the response of the protein to excess-substrate binding. That is to say, the structure of the BuChE active site changes in response to excess-substrate b...

Ngày tải lên: 20/02/2014, 23:20

... the phenomenon of oligomerization may be general to the whole GPCR family, rather than being restricted to some subgroups of receptors. Hence, oligomerization of receptors belonging to the same ... insert and an EcoRI site at the 3¢ end of the insert, to produce the recombinant plasmids TOPOD2L and TOPOD2S, respect- ively.Inordertoaddanepitopetagtobothreceptorsat their N-termi...

Ngày tải lên: 21/02/2014, 00:20

... region; ETNF-6-H, heavy chain of ETNF-6 mAb; HSA, human serum albumin; hTNF-a, human tumor necrosis factor- a; TNF-a, tumor necrosis factor- a; TNF-b, tumor necrosis factor- b; VIP, vasoactive intestinal ... are on the surface of the hTNF-a protein, enabling easy access to catalytic antibodies. The acces- sibility of the TNF-a molecule to ETNF-6-H to is one of the most im...

Ngày tải lên: 06/03/2014, 22:21

... eliminated by proton- ation of amino acid side chains. The amino acid sequence of human Ngb, with respect to the acid-labile horse and human Mbs, displays a high degree of sub- stitution of charged ... (2004) X-ray structures of the maltose–maltodextrin -binding protein of the ther- moacidophilic bacterium Alicyclobacillus acidocaldarius provide insight into acid stability...

Ngày tải lên: 07/03/2014, 02:20