Báo cáo khoa học: Functional analysis of mutations in UDP-galactose-4epimerase (GALE) associated with galactosemia in Korean patients using mammalian GALE-null cells pdf

... transfected individually or in combination into ldlD cells and immunoprecipitation was carried out with myc antibody using detergent-soluble cell lysates containing the amount of protein indicated. ... UDP-galactose-4- epimerase (GALE) associated with galactosemia in Korean patients using mammalian GALE-null cells You-Lim Bang 1, *, Trang T. T. Nguyen 1, *, T...

Ngày tải lên: 07/03/2014, 00:20

... sub- strate acetyl-S-Ppant-PCP. Interestingly, after 5–10 min of incubation at 37 °C, no further hydrolysis of the remain- ing substrate was observed, indicating clear instability of this enzyme under the ... kDa), which is associated with the production of the secondary metabolite surfactin [12,24]. A total of 11 TEII Srf mutants, including mutations of all strictly conserv...

Ngày tải lên: 30/03/2014, 13:20

... group of GHMP kinases in which proton transfer does not play a major role in the rate determining step of catalysis. That five of the 10 disease-causing mutations resulted in insoluble protein in ... 2003 homoserine kinase, proton transfer is unlikely to play a major role in the rate-determining step of catalysis. Several of the disease-causing mutations are not soluble...

Ngày tải lên: 08/03/2014, 02:20

... °Cor 22 °C), by using an alternative expression host {BL21(DE3)[pLysS]} or by including protease inhibi- tors in the solutions used during purification. Kinetic analysis of disease-causing mutations The ... Crosslinking of wild-type and disease-causing mutants of UDP-galactose 4-epimerase (GALE). Crosslinking was carried out with N-(3- dimethylaminopropyl)-N¢-ethylcarbodiimi...

Ngày tải lên: 23/03/2014, 11:20

... N-terminal region of DEC1, including the bHLH domain, interacted with the C-terminal r egion o f BMAL1 in a mammalian two-hybrid assay. Accordingly, a Fig. 5. Analysis of DEC1 mutants for binding to ... region, including the PAS-B domain of BMAL1, is required for the binding to DEC1, Fig. 4. Identification of the domain of D EC1 that interacts with BMAL 1. Interactions of...

Ngày tải lên: 19/02/2014, 16:20

... FEBS 4505 Functional analysis of a murine monoclonal antibody against the repetitive region of the fibronectin-binding adhesins fibronectin-binding protein A and fibronectin-binding protein B from ... fibronectin. The C-terminal domain of fibronectin-binding protein A is organized into 11 tandem repeats, six of which bind the ligand with high affinity; other sites bind more weakly. Fi...

Ngày tải lên: 06/03/2014, 22:21

... aglycone-binding site of Zm-p60.1 differs distinctly from that of Bgl4:1 and its homologs. These findings offer exciting prospects for comparative analysis of the molecular determinants of substrate ... modeling to obtain insights into the molecular interactions underlying the observed changes in the mutants’ enzymatic kinetics. Modeling was only applicable to F193A because inter-...

Ngày tải lên: 16/03/2014, 04:20

... (C1–3) loops. In addition, GPCRs contain a more or less extended N-terminal domain and C-terminal domain, which are both often involved either in ligand binding or G-pro- tein coupling. Owing to their ... 4B). Localization of the ET-1-binding site In order to determine the ETB region that is essential for binding of ET-1, a series of eight plasmids coding for terminally truncated...

Ngày tải lên: 16/03/2014, 10:20

... DNA-binding protein of the ferritin family 563 nt 20 052 Da Contains domains typical of the ferritin-like superfamily, such as: DNA-binding ferritin- like protein, ferritins and bacterioferritins Putative ... proteins in comparison with those of other actinomycetes revealed a strong conser- vation of motifs in domains 1 and 2 ( 70% identical residues), particularly in the DNA...

Ngày tải lên: 16/03/2014, 18:20

... over 12 min at a flow rate of 1mLÆmin )1 . Quantification was by integration of peak areas using breeze software (Waters, Milford, MA, USA). The MCE activity was determined over a range of enzyme ... of the domains occurs in several different ways, suggesting that the evolution of these proteins probably involved multiple gene duplication, gene fusion and domain swapping events. M...

Ngày tải lên: 23/03/2014, 13:20