Báo cáo khoa học: Polypyrimidine tract-binding protein is essential for early mouse development and embryonic stem cell proliferation potx

... splicing is important for ES cell self-renewal and differentiation [3]. However, the mechanisms by which molecules that Keywords cell cycle; embryonic stem cells; knockout mouse; polypyrimidine tract-binding ... viable, they form compact colonies and exhibit severe defects in cell proliferation without precocious differentiation. Our data clearly demonstrate that PTB...

Ngày tải lên: 07/03/2014, 00:20

... endogenous a-amylase of B. subtilis (AmyE), the halo assays were per- formed with amyE mutant B. subtilis 168 X strains. Subcellular localization of proteins The subcellular localization of proteins in E. coli ... for providing anti-Trigger Factor of E. coli, R. Brimacombe for providing anti-L23 of E. coli, M. Marahiel for providing B. subtilis SG1, and P. Genevaux for providing E....

Ngày tải lên: 16/03/2014, 22:20

... for disks. Results for PapMV disks and CP6–215 disks differed from those for F13L and F13Y disks, and suggest that there is still some RNA associated with recombinant F13L and F13Y disks. This ... three forms were as follows: NLP, 33%; disks, 36%; and monomers, 31%. This current profile differs slightly from the first one that we pub- lished [9]. This is probably because the...

Ngày tải lên: 07/03/2014, 05:20

... Vps4p, are essential for interaction with Vta1p. In addition, we show that one of these motifs is required for a homotypic interaction and for formation of a highly active ATPase complex, but is not ... GAI motif is not essential for Vps4p recruitment to endosomes. The Vps4p GAI motif is not essential for ATPase activity To determine whether the GAI motif in the b do...

Ngày tải lên: 16/03/2014, 13:20

... were treated with 0, 20, 40, 60, 80 or 100 lgÆmL )1 LPS for 24 h and then the RNA and protein were isolated for RT- PCR and western blot analysis. The results demon- strated that LPS induced HBD-2 ... HMGN2 is crucial for LPS-induced HBD-2 expression in A549 cells. The cells were incubated in DMEM with 10% FBS containing 0, 20, 40, 60, 80 or 100 lgÆmL )1 LPS for 24 h and t...

Ngày tải lên: 22/03/2014, 16:20

... Sp3 is essential for postnatal survival and tooth development [18], hematopoiesis [19] and myo- cardial development [20]. The involvement of Sp1 and ⁄ or Sp3 in transcriptional activation is gene-spe- cific. ... in cell lysates was determined and normalized according to Renilla luciferase activity. Statistical analysis Values were expressed as the mean ± standard error. S...

Ngày tải lên: 29/03/2014, 00:20

... with increased risk of cardiovascular disease, the leading cause of death. Therefore, it is important to understand the molecular basis underly- ing obesity and related metabolic diseases in order ... resistance [15]. Here, we focus on the roles of ANGPTL2 and ANGPTL6 ⁄ AGF in obes- ity and related metabolic diseases, and discuss whether these ANGPTLs could be targets for the prev...

Ngày tải lên: 14/02/2014, 22:20

... variable reduction and linear discrimi- nant analysis was performed on sensitive and multiresistant cells spectra in order to establish a diagnostic tool for multiresistant pattern. For each of t ... nd K 562/DNS cells is not significant in the amide protein. This is not surprising as spectra were normalized for equal content of proteins. But a qualitative change of the proteins...

Ngày tải lên: 21/02/2014, 03:20

... model (Brill, 1993). This tagging system is a hybrid system using both statistical training and rule-based training. Rule-based training is performed only on the statistical tagging errors. ... transformation rules dealing with agglutinative characteristics of Korean, and also extends the tagger by using specific transforma- tion rule considering the lexical information of mis...

Ngày tải lên: 08/03/2014, 05:21

... gene and protein mentions in bio- logical texts, and show that incorporating unlabeled data improves the performance of the supervised CRF in this case. 1 Introduction Semi-supervised learning is ... Computational Linguistics and 44th Annual Meeting of the ACL, pages 209–216, Sydney, July 2006. c 2006 Association for Computational Linguistics Semi-Supervised Conditional Random Fi...

Ngày tải lên: 17/03/2014, 04:20