Báo cáo khoa học: Relationship between functional activity and protein stability in the presence of all classes of stabilizing osmolytes ppt

... backbone and the osmolytes leading to the preferential hydration of the protein domain is the driving force of protein stabiliza- tion or folding [3,4]. Furthermore, the effect of osmolytes on the functional ... proteins [3,4], but they also induce refolding of misfolded proteins [5–8] and remove protein aggregation [9–12]. Mechanisms of protein osmolyt...

Ngày tải lên: 07/03/2014, 00:20

... 3177 binding of an N-hydroxyguanidine moiety in the iNOS heme pocket thus roughly involves the same binding energy as binding of the guanidine moiety, and the equilibrium constants are mainly ... NOHA [10], nothing is known about the thermodynamics and kinetics of the binding of guanidines and N-hydroxy- guanidines to iNOS. Removal of the a-amino or a-carboxyla...

Ngày tải lên: 16/03/2014, 22:20

... Lucigenin-amplified chemiluminescence of the xanthine/xanthine oxidase reaction in the presence of 40 lgÆmL )1 of oxy and met derivatives of both hemoglobins. The system contained 0.9 U of xanthine ... R.W., Pennelly,R.R.,Fung,L.W.,Ho,C.,Giannini,I.,Porschke,D.& Winkler, H. (1978) Interactions between the quaternary structure of the globin and the spin sta...

Ngày tải lên: 23/03/2014, 12:20

... Sims’ position, as the buttocks fall to each side, and finger tips of both hands of the investigator are free for gentle eversion of the anal skin with the help of a good lighting [5,14]. A fundamental ... reliable for determining the causes of anal bleeding, anal itch, anal pain or anal burning. The sensitivity, specificity and predictive value of patients’ pos...

Ngày tải lên: 03/11/2012, 11:24

... timing of steps involved in dimer formation and a ppearance of thermostabilization by phosphate has been examined. The role of the cofactor 5¢-phosphate group in the induction of stability and stabil- ization ... formation of an a po-oligomer induced by the b inding of the nucleotide sugar. The presence of maltopentaose (5 m M ) resulted in a moderate 1...

Ngày tải lên: 19/02/2014, 16:20

... diagram of the trunca- tion mutants of CcCac1L. (C) Interaction between CcCac1L and CcLim15 in a yeast two-hybrid assay. The inserts in the activation domain (AD) and DNA-binding domain (BK) ... exonu- clease activity and invades the homologous double- stranded region of the other allele. These steps of homology search and recombination are catalysed by two b...

Ngày tải lên: 07/03/2014, 05:20

... on the PEF domains of both the large and small subunits [33,34]. In the presence of Ca 2+ , subdomains A and C of calpastatin tightly associate with PEF domain IV of the large subunit and domain ... determine which of the other PEF domain-containing calpains form heterodimers and which form homodimers. His6-tagged PEF domains of calpains 1, 3, 9 and 13 were...

Ngày tải lên: 16/03/2014, 04:20

... ITSPOKE: Well The product of the mass of a body and its acceleration remains constant for a given force. What is the relationship between a force act- ing on the box, the mass of the box, and its ... CERT – REJ interaction is explained only by the interaction between Certain and Rej and the in- teraction between Uncertain and Rej. 5 Results - dep...

Ngày tải lên: 17/03/2014, 04:20

... understanding of system capa- bilities. Of the remaining 66% of inputs (2166) which were within the functionality of the system, 68% were in grammar. On the within functionality portion of the ... and pen input, includ- ing both pointing and handwriting (Figure 1). Our application task also differs, focusing on search and browsing of a large database of mov...

Ngày tải lên: 20/02/2014, 12:20

... fumarate reduction; the use of the quinol-binding site inhibitor HOQNO shows that the inhibitor blocks binding of MQH 2 at the Q P site [27]. Study of the reduction and oxidation of the cytochrome has ... four kinetic phases in the reduction of the hemes by menaquinol. According to the quinone type used, the two hemes of the cytochrome are probably involv...

Ngày tải lên: 07/03/2014, 15:20