Báo cáo khoa học: Saccharomyces cerevisiae coq10 null mutants are responsive to antimycin A ppt

... Saccharomyces cerevisiae coq10 null mutants are responsive to antimycin A Cleverson Busso1, Erich B. Tahara2, Renata Ogusucu2, Ohara Augusto2,Jose Ribamar Ferreira-Junior3, Alexander ... converted to q)and q0after cell growth for mitochondrial preparation.There are a number of cellular events that lead to mitochondrial DNA instability in yeast [30]. We canspeculate that changes ... Alexander Tzagoloff4, Alicia J. Kowaltowski2and Mario H. Barros11 Departamento de Microbiologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Brazil2 Departmento de Bioquimica,...

Báo cáo khoa học: Saccharomyces cerevisiae Ybr004c and its human homologue are required for addition of the second mannose during glycosylphosphatidylinositol precursor assembly ppt

... are unable to incorporate [3H]inositol into proteins, and accumulate a GPI intermedi-ate having a single mannose that is likely modiﬁed with ethanolaminephosphate. These data indicate that ... essential Saccharomyces cerevisiae Ybr004c protein as a candidate for the secondGPI a- mannosyltransferase (GPI-MT-II). S. cerevisiae cells depleted ofYbr004cp have weakened cell walls and abnormal ... similar-ity calculated relative to S. cerevisiae sequence.OrganismLength(aminoacids)% Identity ⁄similarityGenBankaccessionnumberFungiCandida albicans 394 37 ⁄ 70 EAK94563Candida glabrata...

Báo cáo khoa học: Saccharomyces cerevisiae Pip2p–Oaf1p regulates PEX25 transcription through an adenine-less ORE ppt

... 5¢-TCGACTCTCCGCCGGAAGAGGGTACCGACTTGTCGGAGGAATTCGGAGTG-3¢ This studyPEX14 ORE1 (RE328) 5¢-AATTCATAGCGGTTTTAATAAGCGCCCGAAAGAG-3¢ This studyPEX14 ORE2 (RE329) 5¢-TCGACTCTTTCGGGCGCTTATTAAAACCGCTATG-3¢ ... 5¢-TCGACCGGATCATCGCGATTAACTCCGG-3¢ This studyPEX5 ORE1-R 5¢-TCGACCGGAGTTAATCGCGATGATCCGG-3¢ This studyPEX5 ADR1-F 5¢-TCGACACTCCGAATTCCTCCGACAAGTCGGTACCCTCTTCCGGCGGAGAG-3¢ This studyPEX5 ADR1-R ... genes (Table 1) are functional. Applica-tion of monoclonal antibodies against Pgk1p served asan internal control for equal loading of protein, whereas anantibody against Cta1p was used to demonstrate...

Báo cáo khoa học: Saccharomyces cerevisiae a1,6-mannosyltransferase has a catalytic potential to transfer a second mannose molecule ppt

... (5¢-CAAGAGGTGGTATTTACTCAGCTATGGATACTATGCTTTTGAA-3¢) and D18 8A- RV (5¢-TTCAAAAGCATAGTATCCATAGCTGAGTAAATACCACCTCTTG-3¢),and the pPICZaA-ScOCH1 as a template. The bothD18 8A mutant and wild-type proteins were expressed asmentioned above. After ... that the contaminants may have a catalyticactivity only toward the substrate (Man10GlcNAc2-PA), where the ﬁrst mannose was added to Man9Glc-NAc2-PA, we puriﬁed Man10GlcNAc2-PA and ... proceduresMaterialsThe Pichia pastoris expression kit was purchased from Invi-trogen Corp. (Carlsbad, CA, USA). a1 ,2-Mannosidase (As-pergillus saitoi) was from Seikagaku Corp. (Tokyo, Japan). a1 ,6-Mannosidase...

Báo cáo khoa học: Saccharomyces cerevisiae Cox18 complements the essential Sec-independent function of Escherichia coli YidC doc

... by a smallcytoplasmic loop and two translocated termini: a lipid-modiﬁedN-terminus and a large C-terminus. To permit immunodetection, anHA tag was attached to the C-terminus. (D) Steady state ... andmitochondrial homologues are ﬂanked by nonrelatedregions. The E. coli core domain is preceded by a largeperiplasmic loop and an additional TM. MitochondrialOxa1 and Cox18 lack this region, but are ... [6,9,10,12]. To examine whether Cox18 is able to participate in this Sec-dependent pathway, we analy-sed the putative contacts between EcCox18 and sub-strates of this pathway that are trapped duringintegration...

Báo cáo khoa học: Coordination of three and four Cu(I) to the a- and b-domain of vertebrate Zn-metallothionein-1, respectively, induces signiﬁcant structural changes doc

... 2007)doi:10.1111/j.1742-4658.2007.05770.xVertebrate metallothioneins are found to contain Zn(II) and variableamounts of Cu(I), in vivo, and are believed to be important for d10-metalcontrol. To date, structural information is available for ... form a distorted chair. TheC-terminal a- domain is characterized by an adaman-tane-like four-metal cluster. Solution structures of113Cd-substituted Cd7MT-2 from rabbit, rat andhuman are available ... together, the initial additions of Cu(I) to eachdomain caused the disappearance of a large set ofNOESY cross-peaks and the parallel appearance ofanother set of cross-peaks, until a clean 2D spectrumbelonging...

Báo cáo khoa học: "Joint Processing and Discriminative Training for Letter-to-Phoneme Conversion" ppt

... approach into a uniﬁed dynamicprogramming framework, we are able to achieve substantial performance gains. Ourresults surpass the current state-of-the-art onsix publicly available data sets representingfour ... for eachnew language. Effective rule-based approaches canbe designed for some languages such as Spanish.However, Kominek and Black (2006) show that inlanguages with a less transparent relationship ... n-gramapproach (Bisani and Ney, 2002) accounts for allthree desiderata, but each operation is informed onlyby a limited amount of left context. The many- to- many classiﬁer of Jiampojamarn et al....

Báo cáo khoa học: HIV-1 gp41 and gp160 are hyperthermostable proteins in a mesophilic environment ppt

... are different from a protein that has evolved to be hyperthermostable as a consequence of the adaptation to a factor other thanelevated temperature.The availability of an increasing number of ... the loop are the reason for aggregation atneutral pH. However, the mutation of an additionalamino acid in the loop appears to be necessary to furtherincrease protein solubility.Amino acid residues ... forward and reverse primers, as follows:forward primer: 5¢-CTCTTTCATGACGCTGACGGTACAGGCC-3¢; reverse primer: 5¢-CCGCTCGAGCTAATGGTGATGGTGATGGTGTGACCCTCCCCCTCCACTTGCCCATTTATCTAA-3¢. The start...

Tài liệu Báo cáo khoa học: "SIMULATING CHILDREN''''S NULL SUBJECTS: A NEARLY LANGUAGE GENERATION MODEL" ppt

... learning Italian, a pro-drop language (Valian, 1991) and Chinese, which allows 'topic-drop' (Wang et. al., 322 1992), as compared to English-speaking children of the same age and ... will also be used to examine why other patterns of output appear in the speech of children learning languages such as Italian and Chinese. Initial findings are that an output generator successfully ... claims that the matrix subject is outside the domain where the pro-drop parameters are applied initially, while Rizzi claims that the matrix CP is considered optional at an early stage in acquisition....

Báo cáo khoa học: "What do we know about medication errors made via a CPOE system versus those made via handwritten orders"

... oferrors made with CPOE systems compared to handwrittenorders. I find in Shulman et al.’s article essential questionsthat are too often glossed over or assumed to have obviousanswers. Their ... complexity of medication prescribingerrorShulman and colleagues assign medication errors into a 12category schema that illuminates the many types ofmedication prescribing errors and, key here, ... with dosages. Idiscuss these issues and their implications for the evaluation ofCPOE systems and of other emerging healthcare technologies.Shulman and colleagues [1] have contributed a thoughtfulstudy...

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