Báo cáo khoa học: The N-terminal hybrid binding domain of RN

Báo cáo khoa học: The N-terminal hybrid binding domain of RNase HI from Thermotoga maritima is important for substrate binding and Mg2+-dependent activity pot

... shows processivity for long RNA ⁄ DNA substrates and loses this processivity on removal of the HBD. Binding to substrate To examine whether the HBD of Tma -RNase HI is important for substrate binding, the binding ... catalytic domain (residues 64–223) of Tma -RNase HI; Tma-ND, N-terminal domain (residues 1–63) of RNase HI from Thermotoga ma...

Ngày tải lên : 06/03/2014, 22:21

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Báo cáo khoa học: The Drosophila jumonji gene encodes a JmjC-containing nuclear protein that is required for metamorphosis pot

... interaction domain of dJmj for these factors is the N-terminal repression domain, which is evolutionarily conserved among Jmj proteins (Fig. 1). Detailed analysis of the role of N-terminal and the ... structures of mouse Jmj, Drosophila Jmj and Lid. The locations of the JmjN domain, JmjC domain, ARID, PHD, AT-hook domain and C5HC2 zinc ﬁn- ger d...

Ngày tải lên : 23/03/2014, 07:20

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Báo cáo khoa học: The natural mutation by deletion of Lys9 in the thrombin A-chain affects the pKa value of catalytic residues, the overall enzyme’s stability and conformational transitions linked to Na+ binding pdf

... single band of ~ 36 kDa for all the thrombin forms. Effect of pH on thrombin amidase activity and binding of the inhibitor a-NAPAP to the thrombin active site The effects of pH (5.5–10) on the hydrolysis ... flexibility of the environment of Trp207 and 29 may significantly affect the intrinsic fluorescence of the DK9 variant, the higher the surface exp...

Ngày tải lên : 07/03/2014, 12:20

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Báo cáo khoa học: The variable C-terminal extension of G-protein-coupled receptor kinase 6 constitutes an accessorial autoregulatory domain ppt

... Analysis of the genomic organ- ization of the mouse GRK6 gene showed that the four mRNAs are generated by alternative RNA splicing from a single gene. Three of the GRK6 isoforms, GRK6-A, -B and ... Three isoforms, mGRK6-A to mGRK6-C differ in their C-terminal-most portion, which is known to mediate membrane and ⁄ or receptor interaction and regulate the activity...

Ngày tải lên : 07/03/2014, 12:20

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Báo cáo khoa học: Studies on structure–function relationships of indolepyruvate decarboxylase from Enterobacter cloacae, a key enzyme of the indole acetic acid pathway ppt

... analysis of substrate speciﬁcity and cofactor binding of EcIPDC. The enzymatic conversion of all substrates studied in this work (pyruvate, the native substrate of PDC, benzoylformate, the native substrate ... addition of the cofactors. The enzyme is destabilized at low ionic strength. The stability of EcIPDC in aqueous solutions is higher than that of...

Ngày tải lên : 08/03/2014, 02:20

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Báo cáo khoa học: The proximity between C-termini of dimeric vacuolar H+-pyrophosphatase determined using atomic force microscopy and a gold nanoparticle technique ppt

... translocation of protons from the cyto- sol to the vacuolar lumen, at the expense of PP i hydrolysis, for the storage of ions, sugars, and other metabolites. Dimerization of V-PPase is necessary for ... surface. The volume of the V-PPase homodimers (V m ) in the reconstituted membrane was estimated using the height of the protein protrusion and the...

Ngày tải lên : 16/03/2014, 02:20

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Báo cáo khoa học: The N-terminal cysteine pair of yeast sulfhydryl oxidase Erv1p is essential for in vivo activity and interacts with the primary redox centre doc

... shown for the Erv2 protein, the dimer formation depends on an interaction of the CGC motif with the CXXC motif of the opposite monomer. The short form of the Erv1 protein, which lacks the N-terminal ... serine destroys the disulﬁde bridge and the introduction of a polar group probably interferes with binding of the adenine portion of the FAD that i...

Ngày tải lên : 17/03/2014, 10:20

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Báo cáo khoa học: The allene oxide cyclase family of Arabidopsis thaliana – localization and cyclization ppt

... an inhibitor of AOC (see below), is essential for binding to AOC from corn [18] is consistent with the observed binding position in corn AOC [23]. Any shift of the epoxy group would inhibit the formation ... nature of the side chains of Asn and Leu and steric con- straints imposed by the two methyl groups of Leu on the substrate. The ﬁnding that the...

Ngày tải lên : 23/03/2014, 07:20

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Báo cáo khoa học: The multifarious short-term regulation of ammonium assimilation of Escherichia coli: dissection using an in silico replica pdf

... of ammonium. This partial robustness of the net ammonium assimilation ﬂux is achieved by the active regulation of the system, which is dominated by the adenylylation of GS. The constancy of the ... prediction is based upon the comparison of the calculated behaviour of our in silico ATase, PII and UTase mutants. The removal of either one of the thr...

Ngày tải lên : 23/03/2014, 13:20

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Báo cáo khoa học: The )148 to )124 region of c-jun interacts with a positive regulatory factor in rat liver and enhances transcription Dipali Sharma*, Sujata Ohri and Aparna Dixit ppt

... amounts of divalent cations are necessary for the formation of complex between RLjunRP and the )148 to )124 region of c-jun, and so the optimum amount of MgCl 2 required for complex formation was then ... India is duly acknowledged for the Senior Research Fellowships to D.S. and S.O. The technical assistance of S. Singh is sincerely appreciated. The anima...

Ngày tải lên : 23/03/2014, 20:22

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