Báo cáo khoa học: Transmembrane helix 12 plays a pivotal role in coupling energy provision and drug binding in ABCB1 pot
Báo cáo khoa học: Transmembrane helix 12 plays a pivotal role in coupling energy provision and drug binding in ABCB1 pot
... The Authors Journal compilation ª 2010 FEBS 3985 Transmembrane helix 12 plays a pivotal role in coupling energy provision and drug binding in ABCB1 Emily Crowley1, Megan L. O’Mara2, Ian D. ... the transmembrane domains and nucleotide -binding domains(NBDs) of ABCB1. The present investigation further addressed the role ofTM12 in ABCB1 by characterizing its topography during the multidrugefflux ... atomic-scale model of human ABCB1. Taken together, these results indicate thatTM12 plays a key role in the progression of the ATP hydrolytic cycle in ABCB1 and, in particular, in coordinating conformational...
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Báo cáo khoa học: Acylation of lysophosphatidylcholine plays a key role in the response of monocytes to lipopolysaccharide ppt
... utilized two acyltransferase inhibitors.SK&F 98625, originally described as a CoA-independentacyltransferase inhibitor [16], was included as it inhibitsboth LPCAT and LPAAT in MonoMac 6 cells ... level of U 1A mRNA was similar in all samples asshown in Fig. 4A. This indicated equal extraction efficiency and that SK&F 98625 was not a general transcriptioninhibitor. There was a background ... vials and radioactivity estimated in Optisafe ÔHisafeÕ scintillant(Fisons, Loughborough, UK) using a LKB Wallac 121 1betacounter. Automatic quench correction was made by theexternal standard channels...
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Tài liệu Báo cáo khoa học: Synchronization of Ca2+ oscillations: a capacitative (AC) electrical coupling model in neuroepithelium pptx
... withF-actin via b-catenin and a- catenin; b-catenin bindsto cadherin and a- catenin, which in turn interacts withF-actin (Fig. 3A) [24]. Thus b-catenin plays a pivotal role in the regulation of ... in intracellular [Ca2+], as described below.Synchronous increases in intracellular [Ca2+] and disassembly of cadherin–catenin complexesThe cytoplasmic domain of cadherin interacts withF-actin ... University, Kashihara, JapanStructural organization of intracellularCa2+stores and coupling modesof Ca2+increaseChemical coupling and DC electrical coupling The lumen of the endoplasmic reticulum...
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Tài liệu Báo cáo khoa học: Proteolysis of Pseudomonas exotoxin A within hepatic endosomes by cathepsins B and D produces fragments displaying in vitro ADP-ribosylating and apoptotic effects doc
... occurring mainly within domain III ofETA -A. Cell-free endosomes preloaded in vivo with ETA intraluminallyprocessed and extraluminally released intact ETA and ETA -A in vitro in a pH-dependent and ATP-dependent ... (h)AEEAFDLWNECAKACVLDLKDGVRSSRMSVDPAIADTNGQGVLHYSMVLEGGNDALKLAIDNALSITSDGLTIRLEGGVEPNKPVRYSYTRQARGSWSLNWLVPIGHEKPSNIKVFIHELNAGNQLSHMSPIYTIEMGDELLAKLARDATFFVRAHESNEMQPTLAISHAGVSVVMAQTQPRREKRWSEWASGKVLCLLDPLDGVYNYLAQQRCNLDDTWEGKIYRVLAGNPAKHDLDIKPTVISHRLHFPEGGSLAALTAHQACHLPLETFTRHRQPR2791ETA-B280GWEQLEQCGYPVQRLVALYLAARLSWNQVDQVIRNALASPGSGGDLGEAIREQPEQARLALTLAAAESERFVRQGTGNDEAGAANADVVSLTCPVAAGECAGPADSGDALLERNYPTGAEFLGDGGDVSFSTRGTQNWTVERLLQAHRQLEERGYVFVGYHGTFLEAAQSIVFGGVRARSQDLDAIWRGFYIAGDPALAYGYAQDQEPDARGRIRNGALLRVYVPRSSLPGFYRTSLTLAAPEAAGEVERLIGHPLPLRLDAITGPEEEGGRLETILGWPETA -A LAERTVVIPSAIPTDPRNVGGDLDPSSIPDKEQAISALPDYASQPGKPPREDLK613 A BFig. ... of intact ETA, in vivo association of the catalytic ETA -A sub-unit and low molecular mass ETA -A fragments was observed in theendosomal apparatus. After an in vitro proteolytic assay with an...
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Tài liệu Báo cáo khoa học: Solution structure of hirsutellin A – new insights into the active site and interacting interfaces of ribotoxins docx
... N-terminal hairpin in HtA isintermediate between those in RNse T1 and a- sarcin,having 20 amino acids in HtA, 26 in a- sarcin and 12 in RNase T1. From a functional point of view, thisreduction in length ... in HtA replaces a tyrosine of a- sarcin, and the aromaticring of F126, close to the catalytic H113, replaces a leucine side chain in a- sarcin in a similar arrangementto that found in RNase T1. ... unique among ribotoxins. Compared to a- sarcin, HtA has anaspartate group, D40, replacing a tyrosine, and the aromatic ring of F126,located in the leucine ‘environment’ close to the catalytic...
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Báo cáo khoa học: Gc recruitment system incorporating a novel signal amplification circuit to screen transient protein-protein interactions pot
... the targetZI3 1A gene was amplified by PCR with primers 5¢-AAATATAAAACGCTAGCGTCGACATGGCGC-3¢ and 5¢-AGCGTAAAGGATGGGGAAAG-3¢. The final ratio of targetcells was determined by counting the number ... asvarious mating responses, including global changes in transcription, a reporter gene assay and mating selec-tion are available (Fig. 1A) [12] .Unlike stable interactions, however, transientinteractions ... 67–79.8 Takesako K, Ikai K, Haruna F, Endo M, ShimanakaK, Sono E, Nakamura T, Kato I & Yamaguchi H(1991) Aureobasidins, new antifungal antibiotics.Taxonomy, fermentation, isolation, and properties.J...
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Báo cáo khoa học: Inhibition of PI3K/Akt partially leads to the inhibition of PrPC-induced drug resistance in gastric cancer cells pdf
... CTAGAAAAAGTTGCTGTACTCATCCATGTGTCATGGATGAGTACAGCAAPrPCRNAi2 Sense TTTGGTGATACACATCTGCTCAACATGAGCAGATGTGTATCACCTTTTTAntisense CTAGAAAAAGGTGATACACATCTGCTCATGTTGAGCAGATGTGTATCACAkt RNAi Sense TTTGTAGTCATTGTCCTCCAGCACAGCTGGAGGACAATGACTACTTTTTAntisense ... CCCAAGCTTGGGATGGCGAACCTTGGCTGCTAntisense CGGGATCCTCCCACATCAGGAAGATGAGGAPrPCRNAi1 Sense TTTGTTGCTGTACTCATCCATGACACATGGATGAGTACAGCAACTTTTTAntisense CTAGAAAAAGTTGCTGTACTCATCCATGTGTCATGGATGAGTACAGCAAPrPCRNAi2 ... TTTGTAGTCATTGTCCTCCAGCACAGCTGGAGGACAATGACTACTTTTTAntisense CTAGAAAAAGTAGTCATTGTCCTCCAGCTGTGCTGGAGGACAATGACTAMDR-1 Sense CTCGAGGAATCAGCATTCAGAntisense AGATCTCTTTGAGCTTGGAAGAGCMDR-1 promoter Sense CTCGAGGAATCAGCATTCAGAntisense AGATCTCTTTGAGCTTGGAAGAGCJ....
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Báo cáo khoa học: Detailed structure of lipid A isolated from lipopolysaccharide from the marine proteobacterium Marinomonas vaga ATCC 27119T pot
... 2 and 2 ¢) and ester linked (at positions 3 and 3¢)(R)- 3-hydroxy and (R)-3-acyloxy fatty acids [7]. On the otherhand, according t o data available so far, lipid A structuralvariants displaying ... suchmolecules.Keywords: fast-atom bombardment MS (FAB-M S); lipid A; marine proteobacteria; Marinomonas vaga;NMR.Gram-negative bacteria, along with classical membranelipids based on glycerol, contain an unusual ... lipopolysaccharidefrom the marine proteobacteriumMarinomonas vagaATCC 27119TInna N. Krasikova1, Natalie V. Kapustina1, Vladimir V. Isakov1, Andrey S. Dmitrenok2, Pavel S. Dmitrenok1,Natalie...
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Báo cáo khoa học: Curcumin suppresses the dynamic instability of microtubules, activates the mitotic checkpoint and induces apoptosis in MCF-7 cells ppt
... 73–75.5 Aggarwal BB, Kumar A & Bharti AC (2003) Antican-cer potential of curcumin: preclinical and clinical stud-ies. Anticancer Res 23, 363–398.6 Dohare P, Garg P, Jain V, Nath C & Ray M ... localization of the kinesin proteinEg5 and induced monopolar spindle formation. Further, curcuminincreased the accumulation of Mad2 and BubR1 at the kinetochores, indi-cating that it activated ... 3437advanced pancreatic cancer [2] and a phase III clinicaltrial in combination with gemcitabine and celebrex forthe treatment of metastatic colon cancer [2]. Curcumininhibits tumor growth in...
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Báo cáo khoa học: Suppression of microtubule dynamics by benomyl decreases tension across kinetochore pairs and induces apoptosis in cancer cells potx
... tension across kinetochore pairs and inducesapoptosis in cancer cellsK. Rathinasamy and D. PandaSchool of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai, IndiaBenomyl ... Inazawa J,Toh H & Nagata S (1998) Molecular cloning and char-acterization of human caspase-activated DNase. ProcNatl Acad Sci USA 95, 9123 – 9128 .51 Skehan P, Storeng R, Scudiero D, Monks A, ... chemotherapy. Similar to taxol and vin-blastine, benomyl also induces apoptosis in HeLa cells,indicating that benomyl may be useful in combinationwith other potent anticancer drugs for the treatment...
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Từ khóa: Nghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngchuyên đề điện xoay chiều theo dạngNghiên cứu tổ hợp chất chỉ điểm sinh học vWF, VCAM 1, MCP 1, d dimer trong chẩn đoán và tiên lượng nhồi máu não cấpđề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDEPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhPhát hiện xâm nhập dựa trên thuật toán k meansNghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Định tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Tìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinChuong 2 nhận dạng rui roBT Tieng anh 6 UNIT 2Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtBÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIMÔN TRUYỀN THÔNG MARKETING TÍCH HỢP