Báo cáo khoa học: Catalytic digestion of human tumor necrosis factor-a by antibody heavy chain pot
Báo cáo khoa học: Catalytic digestion of human tumor necrosis factor-a by antibody heavy chain pot
... determining region; ETNF-6-H, heavy chain of ETNF-6 mAb; HSA, human serum albumin; hTNF-a, human tumor necrosis factor-a; TNF-a, tumor necrosis factor-a; TNF-b, tumor necrosis factor-b; VIP, vasoactive ... 3823–3832 ª 2010 The Authors Journal compilation ª 2010 FEBS Catalytic digestion of human tumor necrosis factor-a by antibody heavy chain Emi H...
Ngày tải lên: 06/03/2014, 22:21
Báo cáo khoa học: Catalytic activation of human glucokinase by substrate binding – residue contacts involved in the binding of D-glucose to the super-open form and conformational transitions ppt
... Activation of glucokinase by D-glucose binding FEBS Journal 275 (2008) 2467–2481 ª 2008 The Authors Journal compilation ª 2008 FEBS 2481 Catalytic activation of human glucokinase by substrate binding ... crystal structures of the two conformational states demonstrate large changes in the main -chain torsion angles of both regions on Glc binding. CR II, which Activation of g...
Ngày tải lên: 30/03/2014, 04:20
Báo cáo khoa học: Catalytic transformations of supercoiled DNA as studied by flow linear dichroism technique doc
... 5. Inhibition of topoizomerase II activity measured by the FLD technique. Comparison of the kinetics of inhibition of reaction of 8 lg of pUC19 DNA with 4 lg of topoisomerase II by etoposide and ... study experiment- Fig. 4. The effect of increasing concentrations of several campothe- cine analogues on the kinetics of topoisomerization of pUC19 DNA (8 lg) by topois...
Ngày tải lên: 30/03/2014, 11:20
Tài liệu Báo cáo khoa học: Reversible tetramerization of human TK1 to the high catalytic efficient form is induced by pyrophosphate, in addition to tripolyphosphates, or high enzyme concentration ppt
... CL, Lu CY, Ke PY & Chang ZF (2004) Perturba- tion of ATP-induced tetramerization of human cytosolic thymidine kinase by substitution of serine-13 with aspartic acid at the mitotic phosphorylation ... Structures of thymidine kinase 1 of human and mycoplasmic origin. Proc Natl Acad Sci USA 101, 17970–17975. 15 Sabini E, Ort S, Monnerjahn C, Konrad M & Lavie A (2003) Stru...
Ngày tải lên: 18/02/2014, 12:20
Báo cáo khoa học: Catalytic mechanism of the primary human prostaglandin F2asynthase, aldo-keto reductase 1B1 – prostaglandin D2 synthase activity in the absence of NADP(H) pptx
... structure of the cofactor-binding site within the catalytic pocket. Discussion Catalytic mechanism of PGDS and PGFS activities of AKR1B1 Mutational analysis of the catalytic tetrad of AKR1B1 and ... analysis of the PGFS and PGDS activities of wild-type AKR1B3. Fig. S2. Mutagenesis analyses of the catalytic tetrad of AKR1B3. Table S1. NADP + -binding affinities of wi...
Ngày tải lên: 14/03/2014, 23:20
Tài liệu Báo cáo khoa học: Autolytic activity of human calpain 7 is enhanced by ESCRT-III-related protein IST1 through MIT–MIM interaction pptx
... were proba- bly derived by limited digestion with other cellular pro- teases, and not by autolysis of mGFP–calpain 7. Enhancement of autolysis of mGFP– calpain 7–Strep by GST–MIM in vitro As we ... BamHI–EcoRI sites of pGST–pHis, a cDNA fragment encoding amino acids 1–165 of calpain 7 (calpain 7MIT) was amplified by using a pair of primers (forward, 5¢-GAG AGATCT Enhancem...
Ngày tải lên: 18/02/2014, 04:20
Tài liệu Báo cáo khoa học: Limited suppression of the interferon-b production by hepatitis C virus serine protease in cultured human hepatocytes pptx
... Recognition of double-stranded RNA and activa- tion of NF-kappaB by Toll-like receptor 3. Nature 413, 732–738. 14 Honda K & Taniguchi T (2006) IRFs: master regulators of signalling by Toll-like ... the activation of IRF-3 by M-pIC treatment is mediated by the TLR3 ⁄ TRIF signaling pathway. We obtained further evidence by examining the status of the phos- phorylation an...
Ngày tải lên: 18/02/2014, 16:20
Tài liệu Báo cáo khoa học: Catalytic mechanism of SGAP, a double-zinc aminopeptidase from Streptomyces griseus pdf
... the presence of D 2 O in solution reduced the catalytic A B Fig. 5. Inhibition of SGAP by phosphate ion. (A) A representative plot of a Lineweaver–Burk plot for determination of the mode of inhibition ... metalloenzymes [53–58]. Inhibition of SGAP by fluoride and phosphate anions was investi- gated by determining the initial rates of the hydrolysis of Leu-pNA as a funct...
Ngày tải lên: 19/02/2014, 02:20
Tài liệu Báo cáo khoa học: Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism docx
... isolated by HPLC and used as substrates for AUH. The AUH assay contained 2 m M of the respective isomers of 3-MG-CoA in a total volume of 25 lL. The reaction was started by addition of AUH (1 ... The production of (Z)-3-MG-5-CoA by Gct is probably due to the possible trans-conformation of the 5-carboxyl group of (Z)-3-methylglutaconate. Table 2. Kinetic constants of AUH...
Ngày tải lên: 19/02/2014, 07:20
Tài liệu Báo cáo khoa học: High activity of human butyrylcholinesterase at low pH in the presence of excess butyrylthiocholine pptx
... general features of the recently determined X-ray structure of human BuChE [7,8]. In particular, most of the essential features of the catalytic site (i.e. a catalytic triad of Ser-His-Glu, an ... the mechanism of action of chymotrypsin: the role of the low barrier hydrogen bond. Biochemistry 36, 4576–4584. 46. Warshel, A. (1998) Electrostatic origin of the catalytic...
Ngày tải lên: 20/02/2014, 23:20