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Báo cáo khoa học: New insights into structure–function relationships of oxalyl CoA decarboxylase fromEscherichia coli pptx

Báo cáo khoa học: New insights into structure–function relationships of oxalyl CoA decarboxylase fromEscherichia coli pptx

Báo cáo khoa học: New insights into structure–function relationships of oxalyl CoA decarboxylase fromEscherichia coli pptx

... on oxalyl CoA decarboxylase of E. coli T. Werther et al.2640 FEBS Journal 277 (2010) 2628–2640 Journal compilation ª 2010 FEBS. No claim to original US government works New insights into structure–function ... stepwiselinear gradient at a flow rate of 1 mLÆmin)1.Determination of the molar absorption coefficient of oxalyl CoA and formyl CoA Synthesized and HPLC-purified oxalyl CoA was dissolvedin 25 mm sodium ... The identity of the purifiedenzyme was confirmed using a combination of tryptic diges-tion and MALDI-TOF mass spectrometry.T. Werther et al. Studies on oxalyl CoA decarboxylase of E. coli FEBS...
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Tài liệu Báo cáo khoa học: Structural insights into the substrate specificity and activity of ervatamins, the papain-like cysteine proteases from a tropical plant, Ervatamia coronaria ppt

Tài liệu Báo cáo khoa học: Structural insights into the substrate specificity and activity of ervatamins, the papain-like cysteine proteases from a tropical plant, Ervatamia coronaria ppt

... characteristics of the Cys-25 ⁄ His-159 interactive system and of the modulatory group of papain: resolution of ambiguity by electronic perturba-tion of the quasi-2-mercaptopyridine leaving group in a new ... here.Modes of binding of E-64 with ervatamin-A andervatamin-CThe modes of binding of E-64 with ervatamin-A andervatamin-C have been analyzed and compared withthe structures of other complexes of ... feasibility of the indus-trial application of an enzyme. Three-dimensionalstructures of multiple enzymes from the same speciescan thus be a useful source of natural variants forinvestigation of properties...
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Tài liệu Báo cáo khóa học: Further insights into the assembly of the yeast cytochrome bc1 complex based on analysis of single and double deletion mutants lacking supernumerary subunits and cytochrome b pdf

Tài liệu Báo cáo khóa học: Further insights into the assembly of the yeast cytochrome bc1 complex based on analysis of single and double deletion mutants lacking supernumerary subunits and cytochrome b pdf

... However, the amounts of core protein 1 and coreprotein 2 were relatively unaffected. Therefore, the absence of subunit 6 appeared to alter the rates of processing of two of the redox subunits ... the lack of immunoreactivity in the latter strain was due to the inability of the truncated protein to insert into and be stable in theinner mitochondrial membrane or lack of detection of thetruncated ... B.H.(1997) The N terminus of the Qcr7 protein of the cytochrome bc1complex is not essential for import into mitochondria inSaccharomyces cerevisiae but is essential for assembly of thecomplex. J....
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Tài liệu Báo cáo khoa học: Complete subunit sequences, structure and evolution of the 6 · 6-mer hemocyanin from the common house centipede, Scutigera coleoptrata pptx

Tài liệu Báo cáo khoa học: Complete subunit sequences, structure and evolution of the 6 · 6-mer hemocyanin from the common house centipede, Scutigera coleoptrata pptx

... notion of the usefulness of hemocyaninsequences for the resolution of the arthropod phylogenetictree [4,6].Myriapod hemocyanin subunit evolution and diversityOne of the most striking features of ... version of SIGNALPV1.1 [28]. The aminoacid sequences of the S. coleoptrata hemocyanins wereadded to a previously published alignment of the arthropodhemocyanin superfamily [4,11] by the aid of GENEDOC2.6[29]. ... openreading frames of the five sequences translate into distinctpolypeptides of 656–685 amino acids (Table 1; Fig. 3).Signal peptides required for the transmembrane excretion into the hemolymph...
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Tài liệu Báo cáo khoa học: Dimer asymmetry and the catalytic cycle of alkaline phosphatase from Escherichia coli doc

Tài liệu Báo cáo khoa học: Dimer asymmetry and the catalytic cycle of alkaline phosphatase from Escherichia coli doc

... The influence of Mg2+on the kinetic properties of APase fromE. coli. The influence of Mg2+on the kinetic properties of APase fromE. coli inthepresenceofaZn2+to dimer ratio of 2 : 1 in 2A2M1Pbuffer, ... )Mg2+.Fig. 5. The influence of Mg2+on the kinetic properties of APase fromE. coli. The influence of Mg2+on the kinetic properties of APasefrom E. coli at a Zn2+to dimer ratio of 4 : 1 in 2A2M1P ... catalysis of bothsubunits.Model representation of the catalytic cyclefor APase fromE. coli A model describing the catalytic mechanism of APasefrom E. coli, based on the results of the kinetic...
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Tài liệu Báo cáo khoa học: X-ray crystallographic and NMR studies of pantothenate synthetase provide insights into the mechanism of homotropic inhibition by pantoate docx

Tài liệu Báo cáo khoa học: X-ray crystallographic and NMR studies of pantothenate synthetase provide insights into the mechanism of homotropic inhibition by pantoate docx

... wereport the results of the structural studies of the panto-ate-bound form of the N-terminal domain of E. coli PS (nPS). The structure and substrate-binding interac-tions of E. coli nPS have been ... effects of one of the substrates, i.e. pantoate [8,9]. One of theinteresting features of the plant PSs is the presence of a conserved 24 residue insertion in the sequence [9].This sequence of amino ... using thestructures of PSs from E. coli and M. tuberculosis.Structural analysesAnalysis of the sites of substrate binding from NMR data,and structural comparison of the E. coli, M. tuberculosisand...
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Tài liệu Báo cáo khóa học: Trichostatin A reduces hormone-induced transcription of the MMTV promoter and has pleiotropic effects on its chromatin structure pptx

Tài liệu Báo cáo khóa học: Trichostatin A reduces hormone-induced transcription of the MMTV promoter and has pleiotropic effects on its chromatin structure pptx

... software.ResultsTrichostatin A has pleiotropic effects on MMTVtranscriptionPools of oocytes were injected with 5 ng GR mRNA into thecytoplasm, followed by intranuclear injection of 1 ng of sspMMTV:M13 ... deacetylatedwhereas the acetylation of both H3 and H4 of nuclesomeF was increased [42]. Addition of TSA resulted in only aninsignificant increase of the acetylation level of histone H4 inthe nucleosome ... was added to some of the pools immediately after DNA injection to assemblechromatin in the presence of TSA, this is referred to as earlyTSA (E). Hormone induction of half of these pools wasperformed...
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Tài liệu Báo cáo khóa học: New activities of a catalytic antibody with a peroxidase activity ppt

Tài liệu Báo cáo khóa học: New activities of a catalytic antibody with a peroxidase activity ppt

... value (e )of1 .49· 105M)1Æcm)1at407 nm for MP8. In the case of BSA, 6 mol of MP8 werebound per mol of protein, whereas in the case of KLH, 22 mol of MP8 were bound per 100 000 g of protein. ... insertingMP8 into 3A3 [2]. They are consistent with the insertion of the MP8–Fe(II)–RNO complex into a hydrophobicpocket with no change of the Fe(II) spin state and noreplacement of any of the two ... themaximum of absorption of the MP8–Fe(II)–RNO complex,as a function of time.S-oxidation of thioanisole by various oxidants catalyzedby MP8 or the antibody–MP8 complexOptimization of reaction...
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Tài liệu Báo cáo khoa học:

Tài liệu Báo cáo khoa học: "Understanding the Semantic Structure of Noun Phrase Queries" pptx

... the Semantic Structure of Noun Phrase QueriesXiao LiMicrosoft ResearchOne Microsoft WayRedmond, WA 98052 USAxiaol@microsoft.comAbstractDetermining the semantic intent of webqueries not only ... se-mantic understanding of noun phrase queries. As-suming that the intent class c ∈ C of a query isknown, we cast the problem of extracting the se-mantic structure of the query into a joint segmen-tation/classification ... types of lexicons leveragingdatabases and query logs for each intent class.The first type of lexicon is an IH lexicon com-prised of a list of attribute names for the intentclass, e.g., “box of ce”...
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Tài liệu Báo cáo khoa học:

Tài liệu Báo cáo khoa học: "Learning Hierarchical Translation Structure with Linguistic Annotations" ppt

... packed forests. In Pro-ceedings of the Joint Conference of the 47th AnnualMeeting of the ACL and the 4th International JointConference on Natural Language Processing of theAFNLP, pages 558–566, ... grammars: Soft-ening syntactic constraints to improve statistical ma-chine translation. In Proceedings of Human LanguageTechnologies: The 2009 Annual Conference of theNorth American Chapter of the ... disconnected from therest of the derivation.While in a decoder this is somehow mitigated bythe use of a language model, we believe that theweakness of straightforward applications of SCFGsto model...
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