Báo cáo khoa học: Molecular metamorphosis in polcalcin allergens by EF-hand rearrangements and domain swapping docx

... 2010 FEBS Molecular metamorphosis in polcalcin allergens by EF-hand rearrangements and domain swapping Iris Magler 1 , Dorota Nu ¨ ss 1 , Michael Hauser 2 , Fatima Ferreira 2 and Hans Brandstetter 1 1 ... induce oligomerization transitions of Bet v 4. The changes in the quaternary structure, termed molecular metamorphosis, are induced and controlled by a combi...

Ngày tải lên: 06/03/2014, 11:20

... the spleen and lungs by homogenizing tissues in 5 mL of NaCl ⁄ P i containing 0.05% Tween-80, and the number of viable bacteria in the organs of infected mice were determined by plating serial dilutions ... each strain in a final volume of 500 lL. The actual CFUs of each strain in both inocula were determined by plating serial dilutions on 7H11 plates containing either Km or...

Ngày tải lên: 23/03/2014, 09:20

... [11], resulting in depolarization and a sequence of injury- induced action potentials [12]. The calcium in ux and bystander activation of intra-axonal proteases and cytoskeleton remodelling underlie ... metabolism and protein synthesis [43,44] resulting in polyamines such as putrescine, spermine and spermidine show a strong enhancing effect on neurite outgrowth both in vitro...

Ngày tải lên: 30/03/2014, 16:20

... remain before we can solve the interaction at the atomic level and understand t he role of the threonine side chains in ice binding. The contradiction between the higher activity demonstrated by ... s tructural elements in LpxA that loop out from individual coils and act as ligand binding sites. SbwAFP, in contrast, is essentially a free- standing b-helix with a C-terminal cap. Th...

Ngày tải lên: 19/02/2014, 16:20

... the interaction with IRF-4 and hence might be the equivalent of Pin1’s WW domain, whereas its catalytic domain could induce structural changes in the N-terminal proline-rich domain of IRF-4. In ... Pin1 WW domain and the amyloid peptide will be necessary, and might form a novel starting point for the development of WW domain inhibitors. Even more surprising is the recent fin...

Ngày tải lên: 07/03/2014, 05:20

... correctly interpreted or not by integrating the user’s current ASR accuracy and barge -in rate. 2.2 Estimating ASR Accuracy by using Implicitly Supervised Labels To perform runtime prediction, we use informa- tion ... Predicting Errors by Using Barge -in Rate and ASR Accuracy We predict the errors in barge -in utterances by us- ing a logistic regression function: P = 1 1+exp(...

Ngày tải lên: 23/03/2014, 17:20

... cardosin A-binding protein. The interaction was confirmed by coimmunoprecipitation studies and pull-down assays. To investigate the structural and molecular determinants involved in the inter- action, ... present in the binding assays, and this complex disruption resulted in C2 domain cleavage by Fig. 5. Cardosin A interacts with the C2 domain of PLDa. Pull-down assays fo...

Ngày tải lên: 30/03/2014, 11:20

... used in chloroplasts, assisted by the single polypeptide of NTRc, which supports the functioning of a complete NADP–Trx system using the NTR and Trx domains located at the N-terminal and C-terminal ... peroxiredoxins that, in combina- tion with complementary studies of forward and reverse genetics, have gen- erated stimulating molecular models to explain how this enzyme integrat...

Ngày tải lên: 07/03/2014, 00:20

... substrates, in particular at the acceptor site, including deoxycytidine kinase, human mitochondria thymidine kinase 2 and herpes thymidine kinase 1 and 2 [27–29]. Only human deoxycyti- dine kinase ... kinases are globular proteins with a Ôcore domain made of a five-stranded parallel b-sheet surrounded by eight helices. The protein is made of two subdomains, each containing a nucleotid...

Ngày tải lên: 08/03/2014, 02:20

... it first determines a set of k nearest neigh- bors according to the similarity metric and as- Learning Accuracy (Hahn and Schattinger 1998) as an evaluation measure is described in Section 3. 2 We ... a noun in multiple classes was allowed. The experiments were conducted using ten- fold cross-validation. The nouns present in the constructed classes were divided into a training set...

Ngày tải lên: 08/03/2014, 21:20