Báo cáo khoa học: Role of the C-terminal extension in a bacterial tyrosinase Michael Fairhead and Linda Thony-Meyer doc

... Research. Journal compilation ª 2010 FEBS 2095 Role of the C-terminal extension in a bacterial tyrosinase Michael Fairhead and Linda Tho ¨ ny-Meyer EMPA, Swiss Federal Laboratories for Materials ... the presence of such a bond in N. crassa tyrosinase [15] and in molluscan haemocyanins [28]. The arrangement of a core tyrosinase domain followed by...

Ngày tải lên: 06/03/2014, 11:20

... engi- neered in each plasmid at the junction between the N-terminal and C-terminal domain sequences of each protein. The chimeric protein composed of the N-ter- minal domain of hoPL and of the C-terminal ... whatever the nature of the N-terminal domain. Moreover, the C-terminal domain of hoPLRP2 was not able to bind colipase even in the presence of...

Ngày tải lên: 16/03/2014, 06:20

... archaea, and there is also variation within bacteria. We also focused on the lysine-degradation pathway because the substrates and intermediates of the pathway are structurally sim- ple, and so the ... Schematic drawing of reactions catalyzed by aminovalerate aminotrans- ferase (PA0266) and glutarate semialdehyde dehydrogenase (PA0265). (B) Activity of PA0265 and PA026...

Ngày tải lên: 07/03/2014, 09:20

... ⁄ Abl and ⁄ or phosphoinositide-3-kinase may also activate the small Rho GTPases Cdc42 and Rac1, and binding of CagA PY to Grb2, or Shp2 may regulate proliferative and pro -in ammatory signalling ... domain in the amino-termi- nal region of CagA [28]. In particular, CagA induces the ubiquitination and degradation of RUNX3, thereby extinguishing its ability to inhibit...

Ngày tải lên: 14/02/2014, 19:20

... Each of these variants retains a catalytic activity against yeast RNA comparable with that of parent mBS, indicating that a native conformation is present. A further indication of the similarity ... 5¢-GAGTGCGGCC GCAAGCTTGGGCTG-3¢, had an estimated T m of 82 °C. The reverse flanking primer sequence, 5¢-ATATACA TATGAAAGAAAG-3¢, had a calculated T m of 42 °C. The mutageni...

Ngày tải lên: 16/03/2014, 23:20

... and drugs taken up into cells accumulate in the cytoplasmic leaflet prior to being released into the cytoplasm. In the latter case, Pgp functioning as a flippase will have the added advantage of ... plasma mem- brane into the cytoplasm occurs at a rate similar to that of the flip-flop of dye across the plasma mem- brane. In the case where the release into the...

Ngày tải lên: 29/03/2014, 08:20

... and a sequence that can accelerate the maturation of ACP into HA are both present in a given SP, the competing reactions of ACP maturation and ACP sequestration may make the formation of CPNs as ... inhibition of calcification in soft and mineralized tissues, the extracellular matrix and a wide range of biofluids, including milk and blood. Abbreviations ACP...

Ngày tải lên: 29/03/2014, 23:20

... lead to a significant change in stability, the additional elimin- ation of the positive charge K8 in the double mutants D7 2A ⁄ K8D and D7 2A ⁄ K 8A resulted in an increased apparent stability against ... that its thermal stability was affected in a similar way. Electrostatic interactions play a critical role in guiding and stabilizing functional protein–pro- t...

Ngày tải lên: 30/03/2014, 20:20

... LH1 a and b bands, a band attributable to the mutated PufX is clearly visible. In lane 4 a faint band, attributable to PufXD 2)19 is indicated by an arrow, while no band, except those of LH1 a and ... confidence interval in the determined value of the lag was obtained by using an F-statistic to determine the probability p of a particular fractional increase in...

Ngày tải lên: 31/03/2014, 09:20

... are involved in the signal transduction pathway for P-LAP translocation. Moreover, reagents that increase intracellular cAMP also activate the translocation of P-LAP, indicating that AVP stimulates ... revealed that the increase in the surface level of P-LAP was detectable at 30 min after AVP addition (Fig. 3C). We also examined the AVP-mediated increase in the cell surf...

Ngày tải lên: 08/03/2014, 02:20