... Escherichia coli
phosphotransacetylase structural domains and analysis
of key compounds involved in activity regulation
Valeria Alina Campos-Bermudez, Federico Pablo Bologna, Carlos Santiago Andreo and
Marı
´
a ... is
located in the C-terminal domain, the E. coli Pta
N-terminal domain is involved in stabilization of the
hexameric native structure, in...
... study of the role of recombination in
mtDNA inheritance in S. cerevisiae showed that budding
cells were enriched in linear monomers of mtDNA. It was
proposed that in addition to a role in initiating ... species.
The role of Ydc2 for maintaining the content of mtDNA
in fission yeast is not clear. Apart from DNA repair,
recombination could be involved in the initiation...
... This indicates
that in vitro binding of a dimer requires the presence of a
DNA b inding domain in each i nteracting protein.
This was f urther investigated using a carboxy terminal
deletion of ... to
binding site specificity, nuclear localization, iden tification of
DNA– a nd protein–protein binding domains it is shown
that efficient DNA binding of a h eterodimer requires two
DNA...
... Shen JR, Inoue Y & Sherman LA
(1994) Role of disulfide linkage and putative intermole-
cular binding residues in the stability and binding
of the extrinsic manganese-stabilizing protein to the
photosystem ... & Yocum CF
(1994) Role of disulfide linkage and putative intermole-
cular binding residues in the stability and binding of the
extrinsic manganese-stabilizing p...
... HSEs in the
promoter regions of hsp genes [19]. In addition, an
in ammatory cytokine, interleukin-6, induces the exp-
ression of Hsp70 and Hsp90b via STAT-like binding
A
B
Fig. 5. Activation of ... region between )206
and )187 bp of the hsp70 promoter in vitro and
in vivo, resulting in the induction of Hsp70 expression.
STAT3 is required for the induction of hsp70...
... hand, functional assays of reconstituted
proteins are proposed to be most crucial to yield infor-
mation on the specificity of binding of particular vs.
bulk lipids, the number of their binding ... structures of integral
membrane proteins have revealed structurally diverse
annular, nonannular, single leaflet and laterally con-
nected lipid-binding sites [1–4]. Each of the multip...
... higher.
Quenching of protein tyrosine fluorescence
by acrylamide
A fluorescence spectrum of intrinsic tryptophan and
tyrosine residues in proteins can be a good conforma-
tional probe. In particular, ... scattering data were obtained by
using a dynapro Molecular Sizing Instrument (Protein
Solutions Inc., Milton Keynes, UK) at 20 °C. Various con-
centrations of protein solution at pH...
... Results).
Organization of bovine tryptase genes and location
of intron II–V
Intron II–V length of the two genes encoding bovine
tryptases was evaluated by amplification of bovine genomic
DNA, using the following ... to the inhibition by
standard serine protease inactivators, it is worth mentioning
that, similarly to BLCT [11,32], BLT is sensitive to
aprotinin, the trypsin inhibitor...
... 14 after induction. As
lipid droplet-targeting proteins, perilipin and fatty
acid-binding protein (FABP) are expressed at late and
mature stages of lipid droplet formation. Interestingly,
we ... proteins, including
cell death-inducing DFF45-like effector A (CIDEA), cell death-inducing
DFF45-like effector B (CIDEB) and cell death-inducing DFF45-like effec-
tor C (CIDEC) [fat-specific pr...
... Furthermore,
MinCDE (complex of MinC, MinD and MinE pro-
teins) has been shown to inhibit bacterial cell division
by preventing assembly of the Z-ring [23,40,55]. In
addition to the proteinous regulation of ... quantities of protein util-
izing the principle of protein–dye binding. Anal Biochem
72, 248–254.
57 Lu C, Stricker J & Erickson HP (1998) FtsZ from
Escherichia...