Báo cáo khoa học: Cysteine residues exposed on protein surfaces are the dominant intramitochondrial thiol and may protect against oxidative damage docx

... within the mitochondrial matrix, the location of the exposed thiols on the surface of proteins may alsoprevent oxidative damage to the proteins on whichthey are located. To do this, the exposed thiol ... which contrasts with the matrixconcentration for exposed protein thiols of 60–90 mm.Therefore, within the mitochondrial matrix, exposed cysteine residues on the surface of proteins are by far the ... dominant free thiol. Response of exposed mitochondrial protein thiolsto oxidative stress The high concentration of exposed protein thiols within the mitochondrial matrix is consistent with them...

Tài liệu Báo cáo khoa học: Roles of AP-2 transcription factors in the regulation of cartilage and skeletal development doc

... migra-tion and differentiation. Studies with knockout mice demonstrate that a mainfunction of AP-2s is the suppression of terminal differentiation duringembryonic development. Additionally, the ... migration of undifferentiatedmesenchymal cells to regions determined to differenti-ate into bone, followed by aggregation and the forma-tion of mesenchymal condensation [48,49]. Theseresting and ... expres-sion in the face and limbs. In a follow-up study, theyfound that this conserved cis-acting sequence serves tomaintain a level of AP-2a expression that limits the size of the hand plate and the...

Tài liệu Báo cáo khoa học: A synthetic weak neurotoxin binds with low afﬁnity to Torpedo and chicken a7 nicotinic acetylcholine receptors docx

... kaouthia. Considering that the two toxinsdisplay 11 residue differences and that the competitionsystems used (human and rat on one hand, and chicken on the other) are not identical in the two ... subgroup may exert a highly similarbiological function. This may be in contrast to the Wntxsfrom the second subgroup, which display many deviations and few conserved residues (besides the conserved ... in capitalletters below the second base of each codon. The nucleotides that vary among isoforms are indicated (+), the stop codon is shown (*) and the variant residues are in bold.4250 S. L....

Báo cáo khoa học: Versatile regulation of multisite protein phosphorylation by the order of phosphate processing and protein–protein interactions pptx

... phosphorylation on the one hand, and the speciﬁc and versatile regulation of protein activation required in signaling pathways on the other.ResultsMathematical modelWe consider a target protein ... strongly nonlin-ear through the nonlinear dependence of the effectiverate constants (Eqn 3) on the enzyme concentrations and the conservation relations (Eqn 4). This has the remarkable consequence ... binding and the catalysis. K and P denote the free concentrations ofkinase and phosphatase, respectively, and Ln and Qn are the respective dissociation constants for the kinase–target and phosphatase–target...

Báo cáo khoa học: DmGEN shows a ﬂap endonuclease activity, cleaving the blocked-ﬂap structure and model replication fork docx

... replication fork differing in the gap sizes of the lagging strand. The gaps in the RF2,RF3 and RF4 are 8, 4 and 0 bp, respectively. Asshown in Fig. 7C, DmGEN cleaved the lagging strandof the model ... representation of the conserved N and I domainsof some members of the RAD2 ⁄ XPG family. The total number of amino acids in each protein and homology (% identity) between DmGEN and other members are ... We prepared fourderivatives (RF1–RF4) of the model replication fork.RF1 resembles the replication fork that lacks the pro-gressing lagging strand. RF2, RF3 and RF4 are the forms of the normal...

Báo cáo khoa học: Light-harvesting complex II protein CP29 binds to photosystem I of Chlamydomonas reinhardtii under State 2 conditions doc

... phosphoryl-ated. These residues correspond to positions 16 and 32in the sequence of the mature CP29 (Table 1). The third peptide contained one threonine and three serine residues (Fig. 3C). However, the ... reinhardtii cells exposed to State 2 conditions. The b(N-terminal) and y (C-terminal) fragment ions are labelled and the peptide sequences shown. The lower case t and s in the sequencesdesignate ... during the in-gel digestion procedure and the following peptide extraction, as well as the suppressedionization of the phosphorylated peptides in the pres-ence of nonphosphorylated ones [22]....

Báo cáo khoa học: DYNLL/LC8: a light chain subunit of the dynein motor complex and beyond pptx

... Twoﬁve-stranded b-sheets are formed at the dimerization interface, eachcontaining four strands from one monomer and the ﬁfth from the other. Residues not fully conserved in LC8 paralogs and orthologs are ... the motif are the most conservedGln0, the ﬂanking Thr+1 and Thr)1 residues and Asp)4 (Fig. 1C). We depict 11 residues both on the logo and in Tables 1 and 2 since the binding site on ABCDEFig. ... b-sheetcontains four strands from one monomer and a ﬁfthstrand from the other monomer. These sheets are ﬂanked by two pairs of a-helices at the opposite facesof the dimer. Interestingly, the Tctex ⁄ DYNLT...

Báo cáo khoa học: Two CCAAT/enhancer binding protein sites in the cytochrome P4503A1 locus Potencial role in the glucocorticoid response ppt

... administration The time-course variation of the relative concentrations ofC/EBPa mRNAandproteinwasmonitoredintheratliver.In parallel we also followed the accumulation of the CYP3A1 mRNA, upon addition ... B and C denotes the position of the C/EBP containing complex and the arrowhead ÔssÕ the position of the DNA protein complex shifted by the speciﬁc antibody.Ó FEBS 2003 Two C/EBP sites in the ... markedly on the ontological development, and on the hormonal and nutritional status of the animals [2]. Moreover, P450 mono-oxygenases, as major body interfaces, are adaptive enzymeshighly responsive...

Báo cáo khoa học: Interactions of ultraspiracle with ecdysone receptor in the transduction of ecdysone- and juvenile hormone-signaling pdf

... 2005 FEBS 1581We then assessed the effect of overexpression ofdUSP on the transcriptional activation pathways that are induced by 20OHE alone and on the interaction ofJH III and 20OHE. In order ... antibody against dUSP or a monoclonalantibody against the unrelated protein ELAV [47]. The antibody was added 30 min before addition of the probe.After incubation at 4 °C, the binding reaction contentswere ... promoter construct, the resultant slopes of the plots are not the same. The shallower slope for the IR1JHECore promoter construct compared to the DR1bJHE construct shows that the IR1JHECore con-struct...

Báo cáo khoa học: A new highly toxic protein isolated from the death cap Amanita phalloides is an L-amino acid oxidase pdf

... sequence and deduced aminoacid sequence of the toxophallin. The aminoacid translation is under the secondnucleotide of the corresponding codon. The masses of underlined peptides correspondto the ... treatment and cell death did not depend on the activation of the caspase cascade [16]. The mostpronounced destructive changes, namely condensationof nuclear chromatin and DNA fragmentation, wereobserved ... H2O2scavengers may be protective during its action in the organism.In conclusion, in the present study, a novel cytotoxic protein was isolated from the death cap and character-ized. The physico-chemical,...

Xem thêm