Báo cáo khoa học: Amyloid oligomers: spectroscopic characterization of amyloidogenic protein states docx

... MINIREVIEW Amyloid oligomers: spectroscopic characterization of amyloidogenic protein states Mikael Lindgren 1 and Per Hammarstro ¨ m 2 1 Department of Physics, Norwegian University of Science ... recent optical spectroscopic studies of amyloid protein misfolding, oligomerization and amyloid fibril growth. Although amyloid fibrils have been studied using establis...

Ngày tải lên: 06/03/2014, 09:22

... quantitation of microgram quantities of protein util- izing the principle of protein- dye binding. Anal Biochem 72, 248–254. 30 Gill SC & von Hippel PH (1989) Calculation of protein extinction ... NaOH apo Pfr Pr protein 32 P Fig. 6. Autoradiogram of BphP. Autoradiogram after [ 32 P]ATP[cP] labeling, SDS ⁄ PAGE and electroblotting. (A) Autoradiogram of the apo and holo...

Ngày tải lên: 16/03/2014, 18:20

... isolation of membrane protein complexes in enzymatically active form. Anal Biochem 199, 223–231. 31 Walker KW, Lyles MM & Gilbert HF (1996) Catalysis of oxidative protein folding by mutants of protein ... coefficient of a protein. Protein Sci 4, 2411–2423. 41 Creighton TE (1977) Kinetics of refolding of reduced ribonuclease. J Mol Biol 113, 329–341. 42 Lyles MM & G...

Ngày tải lên: 18/02/2014, 11:20

... particularly regarding the assignment of spectroscopic and redox potentials of the NrfA hemes. In addition, the first partial spectroscopic and redox characterization of the NrfH subunit is also presented. ... best combination of the two subunits that match the smallest molecular mass is a 2 b 2 . Overnight incubation of the protein in this detergent led to a decrease of th...

Ngày tải lên: 21/02/2014, 00:20

... of neurodegeneration induced by Alzheimer’s beta -amyloid protein: channel formation and disruption of calcium homeostasis. Brain Res Bull 53, 389–397. 54 Soto C (2003) Unfolding the role of protein ... against the aggregation of misfolded protein. Importantly, how- ever, our findings also suggest the possibility that the suppression of protein aggregation may cause the for- m...

Ngày tải lên: 06/03/2014, 09:22

... (2006) Formation of soluble oligomers and amyloid fibrils with physical properties of the scrapie isoform of the prion protein from the C-terminal domain of recombinant murine prion protein mPrP-(121-231). ... suppression caused by the amyloid- like aggregates of Sup35 [13]. The propagation of [PSI + ]is strictly dependent on the presence of an appropriate amount of Hsp1...

Ngày tải lên: 06/03/2014, 09:22

... these diseases, inclusions of the mutant proteins are found in the neuronal cells of patients and the accumulation of misfolded proteins is considered to be a primary cause of neuronal dysfunc- tion ... the folding of newly synthesized normal proteins. Thus, the overloading of cellular chaperoning capacity by misfolded mutant proteins results in increased mis- folding of normal...

Ngày tải lên: 06/03/2014, 09:22

... putative T-box DNA-binding domain of t-bet, the STAT protein inter- action domain, STAT protein all-alpha domain, STAT protein DNA-binding domain and SH2 domain of stat6, and the zinc-finger domain, ... on adjacent DNA-binding sites, a coiled-coil STAT protein all-alpha domain, which is implicated in other protein protein interactions, a STAT protein DNA-binding domain and an S...

Ngày tải lên: 16/02/2014, 09:20

... quantitation of microgram quantities of protein uti- lizing the principle of protein- dye binding. Anal Biochem 72, 248–254. 42 Runser SM & Meyer PC (1993) Purification and bio- chemical -characterization ... FEBS 3579 characterization and evaluation of HYD Js from Jannaschia sp. CCS1. Characterization of HYD Js To explore the possible cause of the higher specific activi...

Ngày tải lên: 18/02/2014, 08:20

... left lane of the gel. (B) Interactions of nonlabeled, S protein- tagged full- length SmGCN5 with 35 S-labeled, non-S protein- tagged, full-length SmSmad2 or SmSmad4 (5 lL each) in the presence of non-S ... for LacZ filter-lift assays. The development of a blue color through the activation of the yeast LacZ reporter gene is another indication of a protein protein interaction. Fo...

Ngày tải lên: 18/02/2014, 16:20