Báo cáo khoa học: Temporal expression of heat shock genes during cold stress and recovery from chill coma in adult Drosophila melanogaster pdf

... processes involve expression of some Hsps in Drosophila [40,57]. Finally, it has been suggested that expression of Hsps during recovery from cold might result from the thermal stress experienced during ... studied. In the present study, we focused on 11 Dro- sophila melanogaster Hsp genes during the stress exposure and recovery phases. The temporal ge...

Ngày tải lên: 06/03/2014, 09:22

... different from that of many active promoters, which are marked by binding of TATA-binding pro- tein and recruitment of protein phosphatase 2A [67,68]. In mouse lens, HSF4 binds to nonclassical heat shock ... constitutively in cells and binds to and regulates the expression of genes without any apparent stress intervention [1,3]. HSF2 and HSF4 may not be directl...

Ngày tải lên: 18/02/2014, 04:20

... regulation and binding of heat shock factor 1 and heat shock factor 2 to 32 human heat shock genes during thermal stress and differentia- tion. Cell Stress Chaperones 9, 21–28. 44 Ostling P, Bjo ¨ rk ... aid the folding of damaged proteins, and increased accumulation of HSPs is essential for sur- vival of cells exposed to protein-damaging stresses, includin...

Ngày tải lên: 07/03/2014, 00:20

... suggesting that the heterologous expression of genes encoding metallo- proteins can lead to incorrect metal ion incorporation. This is observed in rubredoxin and desulforedoxins where zinc, instead ... transformants. Protein techniques Protein was determined by protein-dye binding with BSA as a standard [47]. Whole cell protein of C. ammoniagenes cells was isolated from 2 mL of...

Ngày tải lên: 15/02/2014, 01:20

... cloning and cold- inducible gene expression of ferritin H subunit isoforms in rainbow trout cells. J Biol Chem 271, 26908–26913. 13 Tang SJ, Sun KH, Sun GH, Lin G, Lin WW & Chuang MJ (1999) Cold- induced ... properties of the tetrameric enzyme may result in fine-tuning of the properties of skeletal LDH-A, which is in line with previously observed kinetic and thermodyn...

Ngày tải lên: 19/02/2014, 02:20

... ferritin expression in nonpermeabilized (thin lines, – saponin) and permeabilized cells (thick lines, + saponin) cells cul- tured without and with iron as indicated. (D) Perls’ staining of cyto- spins ... in iron-rich media showed a further increase in ROS production as deter- mined by an increase in DCFH mean fluorescence intensity (Fig. 2B, thick line). In addition, determi...

Ngày tải lên: 19/02/2014, 16:20

... Blue staining. Table 2. Quantitation and comparison of proteins in GM and WI-38 cells. From the data in Fig. 2 and others, the concentrations of Mcm proteins, ORC2 and PCNA in total protein and chromatin-bound ... products are involved in the higher level of Mcm expression in HeLa cells and cancer cells from human uterine cervix. Although it remains to be det...

Ngày tải lên: 20/02/2014, 23:20

... an inhibitor of c-Jun N-terminal kinase, showed no effect on the DNA binding of c-Fos and c-Jun induced by treatment with ATZ + mercaptosuccinic acid, indicat- ing that JNK is not involved in ... directly, leading to transactiva- tion of AP-1. On the basis of these findings, we next examined the expression and phosphorylation of c-Fos and c-Jun. The total amount of nuclea...

Ngày tải lên: 06/03/2014, 00:21

... function of increasing amount of total proteins in membrane fractions from I7 yeast strain induced at 15 °C, and from yeast expressing SSTR2 as a control. J. Minic et al. Expression of olfactory ... results in overexpression of GAL4, in turn inducing an increase of the expression of the OR gene under control of GAL1. The pJH2 vector contains the URA3-selectable m...

Ngày tải lên: 07/03/2014, 16:20

... acetylation of Ets1 are also unknown, but of future interest, as mutation of the Ets binding motif (m4) results in both a loss of protein binding and the TSA superinduction of Timp-1. Eleven NAD-independent ... the expression of a protein(s) required for induction of the Timp-1 gene. Time course of TSA action upon PMA- and TGF-b1-induced Timp-1 expression The time co...

Ngày tải lên: 16/03/2014, 18:20