... atrophy.(Received 5 August 20 04, rev ised 27 S eptember 20 04,accepted 6 October 20 04)Eur. J. Biochem. 27 1, 45 52 4558 (20 04) Ó FEBS 20 04 doi:10.1111/j.14 32- 1033 .20 04.04419.xTesque) was dissolved ... Frozen cells weresuspended in 100 lL of extraction buffer [20 mMHepes /KOH, pH 7.9, 1.5 mMMgCl 2 ,0.2mMEDTA, 0.5 mMphenylmethanesulfonyl fluolide, 0.5 mMdithiothreitol,0. 42 MNaCl and 25 % ... http://rsb.info.nih.gov/nih-image/).Thermotolerance of cells C3H10T1 /2 cells (7 · 104 cells per well) grown in 24 -wellplates containing collagen-coated coverslips were incubatedwith or without drugs at 37 °C for 1 h. These cells wereheat-shocked...