Báo cáo khoa học: Staying on message: design principles for controlling nonspeciﬁc responses to siRNA pdf

... activation. InitialBackbone chemistry siRNA sequenceEnd featuresKeyFig. 1. Design considerations for controlling nonspeciﬁc responses to siRNA. This ﬁgure summarizes known recognition interactionsbetween ... MINIREVIEW Staying on message: design principles for controlling nonspeciﬁc responses to siRNA Shirley Samuel-Abraham1and Joshua N. Leonard1,21 Department of Chemical ... recogni-tion events and how siRNA might be designed to control immune recognition (Fig. 1). siRNA design considerations siRNA sequenceWhen selecting an siRNA sequence, potency is oftenthe ﬁrst consideration,...

Tài liệu Báo cáo khoa học: Biosynthesis of riboﬂavin Screening for an improved GTP cyclohydrolase II mutant pdf

... aggrega-tion-prone state of the protein. Owing to the resultingcorruption of the optical readout by the stray lightcontribution, it was not possible to perform a detaileddeconvolution of the stopped-ﬂow ... single-turnover conditions, as well as prest eady-state conditions, afforded useful information for comp arison of the wild-type protein with the mutantCons truct E. Speciﬁcally, absorbance progressioncurves ... a total volume of 400 lL. Experiments were per-formed at 30 °C. The reaction was initiated by the additionof GTP to a predetermined concentration (0.017–1.7 mm).The assay was monitored photometrically...

Tài liệu Báo cáo khoa học: "Seeing stars: Exploiting class relationships for sentiment categorization with respect to rating scales" doc

... kernel function.In Proceedings of CoNLL, pages 57–64.Tong, Richard M. 2001. An operational system for detectingand tracking opinions in on- line discussion. SIGIR Work-shop on Operational Text ... classiﬁer’s goal is to opti-mize binary classiﬁcation error, its major concern is to increaseconﬁdence in the positive/negative distinction, which may notcorrespond to higher conﬁdence in separating ... review might fail to take into con-sideration my overall impression of the ﬁlm — which I hope to capture in the grade” (http://www.sover.net/˜ozus/cinema.htm).2One contributing factor may be that...

Báo cáo khoa học: Molecular basis of perinatal hypophosphatasia with tissue-nonspeciﬁc alkaline phosphatase bearing a conservative replacement of valine by alanine at position 406 Structural importance of the crown domain potx

... compilation ª 2008 FEBS 2733the valine residue at position 406 on one subunit mayinteract with the counterpart on the other subunitthrough their long aliphatic hydrocarbon chains, thuscontributing ... were able to substitute for valine, pointing to theindispensable role of residues with a longer aliphatic side chain at position406 of TNSALP. Taken together, this particular mutation highlights ... infantile forms, develop severe defects inskeletal bone mineralization, unequivocally demon-strating that TNSALP is physiologically involved inthe mineralization process of bone. Consistent...

Báo cáo khoa học: Aberrant interchain disulﬁde bridge of tissue-nonspeciﬁc alkaline phosphatase with an Arg433 ﬁ Cys substitution associated with severe hypophosphatasia pdf

... supernatant was then adjusted to ﬁnal concentrationsof 50 mm acetate buffer (pH 5.5), 1% (w ⁄ v) Triton X-100,and 0.1% SDS. Digestion with Endo H (ﬁnal concentration0.2 U ⁄ mL) was carried out ... A gen-eral method for rapid puriﬁcation of soluble versions ofglycosylphosphatidylinositol-anchored proteinsexpressed in insect cells: an application for human tis-sue -nonspeciﬁc alkaline ... strikingly, a distinct pattern wasobtained under nonreducing conditions. In addition to the two molecular forms, a 160 kDa and a 130 kDaform were found only in the cells expressing TNS-ALP(R433C)...

Báo cáo khoa học: "CS NIPER Annotation-by-query for non-canonical constructions in large corpora" pdf

... perspective. Due to their deviation fromthe canonical constructions, they come in a vari-ety of potential construction patterns as exempliﬁedabove. Non-canonical constructions can be expected to be individually ... Association for Computational Linguistics, pages 85–90,Jeju, Republic of Korea, 8-14 July 2012.c2012 Association for Computational LinguisticsCSNIPERAnnotation-by-query for non-canonical constructions ... same propositional content, the orderof information units available through the permissi-ble grammatical constructions offers interesting in-sights into the constructional inventory of a lan-guage....

Báo cáo khoa học: Sodium pump a1 and a3 subunit isoforms mediate distinct responses to ouabain and are both essential for survival of human neuroblastoma docx

... thereason for the differences found for ouabain-inducedsignaling through the a1ora3 isoforms. The recentdemonstration that the a1 isoform is recruited to theplasma membrane via interaction of ... control0255075100125% of controlBFig. 1. Effect of transfection with various RNAi on expression of a subunit isoforms. (A) Expression of a subunit isoforms in SK-N-AS cellswithout and with transfection with ... the a3 isoformcan generate CTS-induced activation of Erk1 ⁄ 2, indi-cating its localization in an environment different to that of the a1 isoform. The basis for these differentenvironments is...

Báo cáo khoa học: "Effective Use of Function Words for Rule Generalization in Forest-Based Translation" pdf

... challenge for current syntax-based statistical machine translation(SMT) systems. On the one hand, there is a ten-dency to integrate richer syntactic information intoa translation rule in order to ... approach to constructing a derivation forest to include multipleinterpretations of target function words. The deriva-tion forest is a hypergraph as previously used in(Galley et al., 2006), to maintain ... alignments for all of the target function words. The consider-ation behind is in order to generate target functionwords in a robust manner. In order to avoid gener-ating too large a derivation forest...

Tài liệu Báo cáo khoa học: Studies on structural and functional divergence among seven WhiB proteins of Mycobacterium tuberculosis H37Rv pdf

... which islikely to be due to conformational differences betweenthe two proteins. The relative cross-reactivity of poly-clonal antibodies was used to probe the conforma-tional variations at the ... due to the presence of antibodies whichare reacting to the linear epitopes or to conserved con-formational epitopes. The data showed that there aresigniﬁcant structural differences among WhiB ... different conditions was measured.The total iron content of the native and in-columnrefolded protein varied between 0.14 and 0.20 atomsper monomer (Table 1). The sub-stoichiometric ironcontent...

Tài liệu Báo cáo khoa học: "Kernels on Linguistic Structures for Answer Extraction" doc

... depends on the design of a classiﬁer that decides if an an-swer correctly responds to the target question. We design a classiﬁer based on SVMs and different ker-nels applied to several forms ... structureof both deﬁnitions and the latent semantics they con-tain (inherent to behavior, disorder, anxiety) are sim-ilar. So for instance, s2appears as a deﬁnition even to someone who only knows what ... reasonwhy our kernels on linguistic structures improve itby 63%, which is a remarkable result for an IR task(Allan, 2000).2 Kernels for Q/A ClassiﬁcationThe design of an answer extractor...

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