Báo cáo khoa học: Staying on message: design principles for controlling nonspecific responses to siRNA pdf

... activation. Initial Backbone chemistry siRNA sequence End features Key Fig. 1. Design considerations for controlling nonspecific responses to siRNA. This figure summarizes known recognition interactions between ... MINIREVIEW Staying on message: design principles for controlling nonspecific responses to siRNA Shirley Samuel-Abraham 1 and Joshua N. Leonard 1,2...

Ngày tải lên: 06/03/2014, 01:23

... aggrega- tion-prone state of the protein. Owing to the resulting corruption of the optical readout by the stray light contribution, it was not possible to perform a detailed deconvolution of the stopped-flow ... single-turnover conditions, as well as prest eady- state conditions, afforded useful information for comp arison of the wild-type protein with the mutant Cons truct E. Specificall...

Ngày tải lên: 18/02/2014, 11:20

... kernel function. In Proceedings of CoNLL, pages 57–64. Tong, Richard M. 2001. An operational system for detecting and tracking opinions in on- line discussion. SIGIR Work- shop on Operational Text ... classifier’s goal is to opti- mize binary classification error, its major concern is to increase confidence in the positive/negative distinction, which may not correspond to higher confid...

Ngày tải lên: 20/02/2014, 15:20

... compilation ª 2008 FEBS 2733 the valine residue at position 406 on one subunit may interact with the counterpart on the other subunit through their long aliphatic hydrocarbon chains, thus contributing ... were able to substitute for valine, pointing to the indispensable role of residues with a longer aliphatic side chain at position 406 of TNSALP. Taken together, this particular mut...

Ngày tải lên: 07/03/2014, 05:20

... supernatant was then adjusted to final concentrations of 50 mm acetate buffer (pH 5.5), 1% (w ⁄ v) Triton X-100, and 0.1% SDS. Digestion with Endo H (final concentration 0.2 U ⁄ mL) was carried out ... A gen- eral method for rapid purification of soluble versions of glycosylphosphatidylinositol-anchored proteins expressed in insect cells: an application for human tis- sue -nonspecific alka...

Ngày tải lên: 16/03/2014, 12:20

... perspective. Due to their deviation from the canonical constructions, they come in a vari- ety of potential construction patterns as exemplified above. Non-canonical constructions can be expected to be individually ... Association for Computational Linguistics, pages 85–90, Jeju, Republic of Korea, 8-14 July 2012. c 2012 Association for Computational Linguistics CSNIPER Annotation-by-...

Ngày tải lên: 16/03/2014, 20:20

... the reason for the differences found for ouabain-induced signaling through the a1ora3 isoforms. The recent demonstration that the a1 isoform is recruited to the plasma membrane via interaction of ... control 0 25 50 75 100 125 % of control B Fig. 1. Effect of transfection with various RNAi on expression of a subunit isoforms. (A) Expression of a subunit isoforms in SK-N-AS cells witho...

Ngày tải lên: 29/03/2014, 09:20

... challenge for current syntax-based statistical machine translation (SMT) systems. On the one hand, there is a ten- dency to integrate richer syntactic information into a translation rule in order to ... approach to constructing a derivation forest to include multiple interpretations of target function words. The deriva- tion forest is a hypergraph as previously used in (Galley et...

Ngày tải lên: 30/03/2014, 21:20

... which is likely to be due to conformational differences between the two proteins. The relative cross-reactivity of poly- clonal antibodies was used to probe the conforma- tional variations at the ... due to the presence of antibodies which are reacting to the linear epitopes or to conserved con- formational epitopes. The data showed that there are significant structural differences...

Ngày tải lên: 18/02/2014, 12:20

... depends on the design of a classifier that decides if an an- swer correctly responds to the target question. We design a classifier based on SVMs and different ker- nels applied to several forms ... structure of both definitions and the latent semantics they con- tain (inherent to behavior, disorder, anxiety) are sim- ilar. So for instance, s 2 appears as a definition even to so...

Ngày tải lên: 20/02/2014, 09:20