Báo cáo khoa học: Active site residue involvement in monoamine or diamine oxidation catalysed by pea seedling amine oxidase doc

... (2011) 1232–1243 ª 2011 The Authors Journal compilation ª 2011 FEBS 1243 Active site residue involvement in monoamine or diamine oxidation catalysed by pea seedling amine oxidase Maria Luisa Di Paolo1, ... fac-tors underlying substrate selectivity still remain to be discovered. With this in view, we examined the kinetic behaviour of pea seedling amine oxidase with cadaverine and hexylamine, the ... the monoamine is driven by hydrophobic interactions. Instead,binding of the diamine is strongly facilitated by electrostatic factors, con-trolled by polar side-chains and two titratable residues...

Tài liệu Báo cáo khóa học: Active-site residues and amino acid speciﬁcity of the bacterial 4¢-phosphopantothenoylcysteine synthetase CoaB pptx

... ofsubstrates by forming energy-rich thioester bonds. Oxo-coenzyme A (which has an ethanolamine residue instead ofa cysteamine residue) cannot fulﬁll this role and may inhibitcell growth. Therefore, ... converted to PPC (in presenceof cysteine) or to PP (in the presence of cysteamine) or to 4¢-phosphopantothenoylcysteine methyl ester (in theFig. 3. Residues that are crucial for the second-half ... conserved in CoaB proteins. Residues of the N-terminal part of the Escherichia coliCoaB domain that are conserved in CoaB (Dfp) proteins from all kingdoms of life and that were examined in the...

Báo cáo khoa học: Active site mutants of Drosophila melanogaster multisubstrate deoxyribonucleoside kinase pot

... beensuggested to be important for the ability of HSV-1 TK tobind and phosphorylate the acyclic guanosine analogues.The corresponding amino acid residue in Dm-dNK is anisoleucine (I29). To investigate ... deoxyribonucleo-side kinase; dCK, deoxycytidine kinase; dGK, deoxyguanosine kinase;TK2, thymidine kinase 2; HSV-1 TK, herpes simplex virus type-1thymidine kinase; dAdo, deoxyadenosine; dCyd, deoxycytidine;dGuo, ... ganciclovir binding that is unique forHSV-1 TK. We have used the structural information toperform site- directed mutagenesis of the residues N28, I29,F114 and Q81 in the Dm-dNK active site. The...

Báo cáo khoa học: Active site residues governing substrate selectivity and polyketide chain length in aloesone synthase docx

... maintains almost iden-tical CoA binding site and the catalytic triad ofCys164, His303, and Asn336 (numbering in M. sativaCHS) (Fig. 2). Furthermore, most of the active site residues including ... located in proximity of the catalytic Cys164 atthe ‘ceiling’ of the active site cavity guides the growing polyketide chain to extend into the buried pocket.I. Abe et al. Active site residues in ... (1998) Anat-omy of protein pockets and cavities: measurement ofbinding site geometry and implications for liganddesign. Protein Sci 7, 1884–1897. Active site residues in aloesone synthase I....

Báo cáo khóa học: Active site residues and mechanism of UDP-glucose dehydrogenase ppt

... covalently bound to an active site lysine residue via an imine linkage [18]. This suggestion wassupported by experiments on the bovine liver enzyme in which the active site cysteine thiol had been ... observed in approximately equal amounts,conﬁrming that the reaction was accompanied by incor-poration of a single solvent-derived oxygen atom. In orderto determine the position of the incorporated18O-atom,both ... and therefore thehydride transfer steps are not rate determining in thisreaction. Site- directed mutagenesis In an effort to further understand the roles of the active site residues in catalysis...

Báo cáo khoa học: Active-site-speciﬁc chaperone therapy for Fabry disease Yin and Yang of enzyme inhibitors pptx

... catalytically fully or partially active. Active- site direc-ted competitive inhibitors are often effective active- site- speciﬁc chaperoneswhen they are used at subinhibitory concentrations. Active- site- speciﬁcchaperones ... ﬁrst domaincontains the active- site formed by the C-terminal endsof the b strands at the center of a barrel. Thirteenamino acid residues were predicted to be directlyinvolved in the interaction ... in favor of proper folding, therebyincreasing intracellular enzyme activity (Fig. 3). Retro-spectively, these enzyme inhibitors could be usefultools for probing and assessing the folding status...

Báo cáo khoa học: Hydrogen bond residue positioning in the 599–611 loop of thimet oligopeptidase is required for substrate selection pdf

... regions lining the substrate-binding site allow the enzyme toconform to substrates of varying structure. This study describes mutantforms of thimet oligopeptidase in which Gly or Tyr residues in ... around the active site residuesof domain I and the loop and Tyr residues in domai-n II supports our initial conformation for the model.Furthermore, the experimental effects observed forTyr605 ... Authors Journal compilation ª 2008 FEBS 5613(the corresponding residue in neurolysin), demon-strated that this residue may be important in governingthe differences in substrate selection by...

Tài liệu Báo cáo khoa học: The bacterium, nontypeable Haemophilus inﬂuenzae, enhances host antiviral response by inducing Toll-like receptor 7 expression ppt

... receptor anddownstream adaptors are involved in TLR7 induction by NTHi. Because TLR2 is important for mediatingNTHi-induced gene transcription, we ﬁrst investigatedthe role of TLR2 in NTHi-induced ... bacterial prod-ucts including peptidoglycan, lipoprotein and lipoara-binomannan [1–5]. The importance of TLR2 in hostKeywordscylindromatosis; mixed infection;nontypeable Haemophilus in uenzae; signaltransduction; ... signaling path-way. Interestingly, CYLD, a novel deubiquitinase, acts as a negative regu-lator of TLR7 induction by NTHi. Our study thus provides new insightsinto a novel role for bacterial infection...

Tài liệu Báo cáo khoa học: "Identifying Agreement and Disagreement in Conversational Speech: Use of Bayesian Networks to Model Pragmatic Dependencies" docx

... ﬁrst performed several empirical analyses in or- der to determine to what extent contextual informa-tion helps in discriminating between agreement anddisagreement. By integrating the interpretation ... baseline algorithm.3.2 Maximum Entropy RankingWe view the problem as an instance of statisti-cal ranking, a general machine learning paradigmused for example in statistical parsing (Collins,2000) ... important in interactions of multiple speak-ers since other speakers have more opportunities toinsert utterances between the two elements of theAP construction (e.g. interrupted, abandoned or...

Báo cáo khóa học: Mechanistic insight into the peroxidase catalyzed nitration of tyrosine derivatives by nitrite and hydrogen peroxide doc

... binding constants to the proteins.According to Schemes 1 and 2, the nitrating speciesproduced by the enzyme is either NO2,formedbycompound I or II and nitrite (pathway A), or Enitrformed by ... Spectroscopic and binding studieson the interaction of inorganic anions with lactoperoxidase.J. Inorg. Biochem. 68, 17–26.35. Gebicka, L. (1999) Kinetic studies on the oxidation of nitrite by horseradish ... The corresponding KnitriteMvalues are connected to theafﬁnity of nitrite for the protein site where it is transformedinto the nitrating species, in the presence of the phenol.Interestingly,...

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