Báo cáo khoa học: Structural and functional aspects of unique type IV secretory components in the Helicobacter pylori cag-pathogenicity island potx

... MINIREVIEW Structural and functional aspects of unique type IV secretory components in the Helicobacter pylori cag-pathogenicity island Laura Cendron 1 and Giuseppe Zanotti 2 1 Department of ... anti- genic factor of the bacterium, is the main signature of the cagPAI-positive strains. Indeed, cagPAI confers H. pylori the capability to express...

Ngày tải lên: 06/03/2014, 00:21

... (Fig. 1C). The 2¢OH of the ribose is hydro- gen bonded to the main chain and the side chain of Asp142 (Fig. 1C). Both hydroxyl groups of the ribose are involved in coordinating a putative Ca 2+ ion. ... com- pared to the spectrophotometric methods. Because the enzymatic activity of PRTFDC1 towards hypoxanthine and guanine is only 0.26% and 0.09%, respectively, o...

Ngày tải lên: 15/02/2014, 01:20

... it to the electron-donating (FNR) and electron- accepting (NOSoxy) components within the NOS dimer. The CaM-binding site (gray box) in the H2 hinge enables CaM to in uence the movements. The same ... rate [111–114]. The FMN–FNR sub- domain hinge (H1 in Fig. 5) is one of the least con- served motifs and is shorter in eNOS than in nNOS. Swapping the H1 hinge...

Ngày tải lên: 18/02/2014, 11:20

... Comparison of rates and kinetic isotope effects using PEG-modified variants and glycoforms of glucose oxidase: the relationship of modification of the protein envelope to C–H activation and tunneling. ... by the driving force, DG 0 , and reorganization energy in the standard way: DG z ¼ DG 0 þ k ÀÁ 2 =4k ð2Þ The driving force dependence of H transfer in the flav...

Ngày tải lên: 18/02/2014, 11:20

... Alanine-scanning of the 50’s loop in the Clostridium beijerinckii flavodoxin: evaluation of additivity and the importance of interac- tions provided by the main chain in the modulation of the ... & Medina M (2004) Role of neighboring FMN side chains in the modulation of flavin reduction potentials and in the energetics of the FMN:apoprotein intera...

Ngày tải lên: 18/02/2014, 11:20

... 168–174 are missing; in chain F, residues 1 and 168–175 are missing. All amino acid residues were present in the E chain, and part of the His-tag was observed in the B chain. A few amino acid residues ... carried out in o [29] and coot [30]. In chain A, residues 1 and 169–176 are missing; in chain B, residues 167–172 are missing; in chain C, residues 1, 2 and 1...

Ngày tải lên: 18/02/2014, 16:20

... hydrolysis of the nucleotide. To investi- gate the in uence of the binding and ⁄ or hydrolysis of ATP on the interaction of the AAA-peroxins, Pex1p and Pex6p carrying mutated ATP-binding sites ... do interact in vivo. We assigned their corresponding binding sites and elucidated the importance of ATP-binding and -hydrolysis of Pex1p and Pex6p for their inte...

Ngày tải lên: 07/03/2014, 16:20

... Ca 2+ binding and homodimerization of Iba1 and Iba2. Furthermore, F-actin binding and cross-linking assays were performed with both human Iba proteins, and their role in bacterial invasion was investigated. Results Crystal ... Ca 2+ coordination. Sedimentation experiments and microscopy detected pronounced, indistin- guishable F-actin binding and cross-linking activity of Ib...

Ngày tải lên: 16/03/2014, 06:20

... with the amino group and the N3 atom of the cytosine (Fig. 1). This study uses site-directed muta- genesis to further explore the contribution of these amino acids interacting with the pyrimidine ... domain, is conserved in NMP kinases. It is used as a rigid platform around which the short a-heli- cal LID domain, situated in the C-terminal moiety, and the NMP-bindi...

Ngày tải lên: 16/03/2014, 10:20

... P Gs in vitro [21], the metabolic origin of the unusual 15R-PGs remained uncertain until our recent report on the cloning and expression of a COX from the R-variety of P. homomalla [22]. The discovery ... tive site residue at position 349, Val in the novel P. homomalla 15S-COX and in all known 15S-specific COX proteins, and an Ile in the 15R- COX [22,31] To de...

Ngày tải lên: 23/03/2014, 12:20