Báo cáo khoa học: Binding of cGMP to the transducin-activated cGMP phosphodiesterase, PDE6, initiates a large conformational change involved in its deactivation ppt

... FEBS Binding of cGMP to the transducin-activated cGMP phosphodiesterase, PDE6, initiates a large conformational change involved in its deactivation Akio Yamazaki 1,2,3 , Fumio Hayashi 4 , Isao Matsuura 5 and ... we investigated whether cGMP binds to Pabc during deactivation of Pabc to Pabcc. Binding of cGMP may be involved in Pabc deact...

Ngày tải lên: 06/03/2014, 00:21

... Wei Table 3. Summary of the changes in SASA in A ˚ 2 and heat capacity changes (DC p ) in calÆmol )1 ÆK )1 calculated for the complex models. DA tot is the change in total accessible surface area, ... formation is an informative measure that can provide insights into the exchange of water during the process. The relations connecting the changes in heat capacity...

Ngày tải lên: 16/03/2014, 06:20

... observed agglutination of bacteria and yeast by hemolin may be due to binding and crossing-linking of these micro-organisms by hemolin. Binding of hemolin to bacteria and yeast cells M. sexta hemolin ... 86%. LTA was more effective than LPS itself as an inhibitor of hemolin binding to LPS, suggesting that LPS and LTAbindtothesamesitesonhemolinandthathemolin may have a hi...

Ngày tải lên: 31/03/2014, 09:20

... conforma- tional changes associated with ATP binding; (c) obtaining insights into the active site contact residues involved in ATP binding; and (d) relating this informa- tion to steady-state enzyme kinetic ... The pseudo-absorbance data were obtained at the same time as the CD data in (A) , reporting on the biphasic heat-induced increase in absorbance. The regression...

Ngày tải lên: 06/03/2014, 00:20

... þ À 1 K d;AMA Á [AMA] ÁÁ ð2Þ which takes into account the concentration of AMA and the K d,AMA of the AMA–porcine odorant binding protein complex (1 lm). General anesthetic binding to a b-barrel protein ... Halothane binding to the b-barrel cavity was determined using fluorescence quenching of Trp16, and a com- petitive binding assay with 1-aminoanthracene. In a...

Ngày tải lên: 07/03/2014, 16:20

... of RNase A and 3¢-nucleotides, and indicate that non-natural fura- nose rings can serve as the basis for more potent inhibitors of catalysis by RNase A. Abbreviations araUMP, arabinouridine 3¢-phosphate; ... contribution of noncata- lytic phosphate -binding subsites to the mechanism of bovine pancreatic ribonuclease A. Cell Mol Life Sci 54, 766–774. 31 Altona C & Sunda...

Ngày tải lên: 16/03/2014, 18:20

... plays a primary role in initiating and maintaining the vascular response to injury. By interacting with HK, kallikrein cleaves off the vasoactive peptide, bradykinin [21–23]. Bradykinin stimulates ... FXIIa binding to the surface or to conforma- tional changes of FXIIa occurring when bound to the surface. Both of these mechanisms may be involved in the protecti...

Ngày tải lên: 23/03/2014, 20:22

... ATP [7] and more recently crystallographically as a large conformational change in the N-terminal nucleotide binding domain of a C-terminal truncated form of Grp94 upon ATP binding [17]. These ... [25]. In that case it was shown crystal- lographically that the ligand binding induced a con- formational change which caused a net decrease in the hydrodynamic...

Ngày tải lên: 30/03/2014, 11:20

... monoclonal Z2 antibody in the presence of increasing concentrations of potassium thiocyanate (ranging from 0 to 2.0 m). After incubation and washing, the antibody binding was mea- sured using a goat ... hydrophobicity in the binding of a pH 4.0 buffer-treated monoclonal IgG antibody to its target antigen To evaluate the types of intermolecular interactions...

Ngày tải lên: 16/02/2014, 15:20

... binding antibodies. The signal intensity of each of the three protein bands was quantified by densitometry. The mean values of the calculated signal intensities were analyzed for each of the N- or C-terminal binding ... post- translational modification involving removal of an N-terminal signal peptide and C-terminal residues in the polypeptide chain and attachment o...

Ngày tải lên: 19/02/2014, 02:20