Báo cáo khoa học: Interaction of the general transcription factor TnrA with the PII-like protein GlnK and glutamine synthetase in Bacillus subtilis potx

... 2011 FEBS 1789 Interaction of the general transcription factor TnrA with the PII-like protein GlnK and glutamine synthetase in Bacillus subtilis Airat Kayumov 1,2 , Annette Heinrich 3 , Kseniya ... blotting. Fig. 6. The interaction of truncated TnrA proteins with GlnK and GS. (A) BIAcore analysis of GlnK and GS binding to wild-type TnrA...

Ngày tải lên: 06/03/2014, 00:21

... analyzed the effect of HSP25 on the polymerization of intact actin and aggregation of heated actin. Interaction of HSP25 with intact actin In the first series of experiments we analyzed the effect of HSP25 ... The quantity of actin in the pellet was plotted against the total quantity of actin in the sample. Results Heat denaturation of actin Before...

Ngày tải lên: 20/02/2014, 23:20

... 17.6 Fig. 5. Insertion of stefins into DOPC and DOPG monolayers. (A) Kinetic traces of the insertion into DOPG lipid monolayers at initial pressure of 5 mNÆm )1 . The proteins were injected into the sub- phase ... signal increase during the injec- tion phase was the largest and there was low dissoci- ation after the end of injection. Discussion The main hypothesis for...

Ngày tải lên: 07/03/2014, 17:20

... decreased interactions, and the ND49 mutant showed 80% loss of interaction with NV. These observations suggest that there is a major interacting domain within the N-terminal 49 amino acids of the SeMV ... ELISA in the absence of the interacting proteins. rMP interacted with NV in both the experi- ments (Fig. 4A,B). Nature of the MP–NV interaction To determi...

Ngày tải lên: 15/03/2014, 00:20

... investigate the basis of the interaction of E2p with E3 in the assem- bly of the pyruvate dehydrogenase multienzyme complex of Bacillus stearo- thermophilus. Thirteen of the 55 amino acids in the PSBD ... describes the interaction of a 15 N-labelled PSBD (residues 119–171) of the B. stearothermophilus E2p polypeptide chain with the dimer of a p...

Ngày tải lên: 16/03/2014, 18:20

... quantities of protein utilizing the principle o f protein dye binding. Anal. Biochem. 72, 248–254. 35. Laemmli, U.K. (19 70) Cleavage of structural proteins us ing the assembly of the h ead of b acteriophage ... o ther inducible factor interacts with R LjunRP bound to the )148 to )124 region, and further enhances c-jun transcription in proliferating liver. Most...

Ngày tải lên: 16/03/2014, 18:20

... C1r 2 –C1s 2 in the C1 complex that hinders the interaction of the C1q subunit with the polysaccharide. According to this hypo- thesis fucoidan should then interact with the hinge region containing the binding ... determine the site of interaction on the protein. For this purpose we took advantage of the binding properties of C1q toward DNA and of an e...

Ngày tải lên: 16/03/2014, 23:20

... characteristic of the TÆT minor- groove than the AÆA minor-groove, in the binding of DAPI [16]. In conclusion, the main cause of the strong inhibitory effect of DAPI on DNA replication in vitro reported in ... fluorescence and UV thermal melting studies. The addition of ATT-template to DAPI solution determines drug fluorescence changes that are distinctive of...

Ngày tải lên: 16/03/2014, 23:20

... both 1 H-NMR and 31 P-NMR data argue for the absence of CTII binding to Pam 2 Gro-PCho. The in uence of CTII binding to MLV of Pam 2 Gro- PGro on the lineshapes of the 31 P-NMR spectra of the phospholipid ... inserted volume of CTII molecule within the micelle and within the membrane increases when the imidazole group of His31 becomes neutral. This is reflect...

Ngày tải lên: 17/03/2014, 03:20

... competence in PDE4 and to play a key role in the binding of competitive PDE4 inhibitors [36]. The side chain of this residue may conceivably p-stack with the purine base of the bound substrate and form ... on the other side of the catalytic pocket, identified by Turko and colleagues, such as Q765 [13]. The removal of part of the catalytic pocket explains...

Ngày tải lên: 17/03/2014, 09:20