Báo cáo khoa học: Characterization of heme-binding properties of Paracoccus denitrificans Surf1 proteins doc
... in Surf1 A. Hannappel et al. 1778 FEBS Journal 278 (2011) 1769–1778 ª 2011 The Authors Journal compilation ª 2011 FEBS Characterization of heme-binding properties of Paracoccus denitrificans Surf1 ... purification of Surf1 Heterologous expression of apo -Surf1 in E. coli was per- formed in the absence of the Paracoccus heme a-synthesiz- ing machinery as described pr...
Ngày tải lên: 06/03/2014, 00:21
... concomitant appearance of other signals in other parts of the spectrum. This result could be a consequence of the paramagnetic properties of Fe(II), or it might arise from the presence of an intermediate equilibrium ... spectra of CyaY titrations. Superimpo- sitions of a reference [ 1 H, 15 N] HSQC spectrum of CyaY (blue) with a spectrum (red) of a 1 : 1 mixture of CyaY...
Ngày tải lên: 18/02/2014, 16:20
... and properties of type I-signal peptidases of Bacillus amyloliquefaciens Hoang Ha Chu, Viet Hoang*, Peter Kreutzmann², Brigitte Hofemeister, Michael Melzer and JuÈ rgen Hofemeister Institute of ... 1,A2). A BLAST search of the nucleotide sequence of the ampli®ed DNA fragment revealed the presence of three ORFs, the deduced proteins having 70, 77, and 67% identity to proteins...
Ngày tải lên: 21/02/2014, 03:20
Tài liệu Báo cáo khoa học: Characterization and functional expression of cDNAs encoding thyrotropin-releasing hormone receptor from Xenopus laevis Identification of a novel subtype of thyrotropin-releasing hormone receptor ppt
... subtypes of TRHR cDNA (xTRHR1, xTRHR2 and xTRHR3) from Xenopus brain. These encode the entire sequences of the proteins. The amino acid sequence of xTRHR1 is very similar (74–78% identity) to that of ... desensitization of the responses. (Lower) Responses of the three xTRHR subtypes to different concentrations of TRH. The maximum current amplitude of each recording was meas- u...
Ngày tải lên: 21/02/2014, 03:20
Báo cáo khoa học: Characterization and cDNA cloning of a clofibrate-inducible microsomal epoxide hydrolase in Drosophila melanogaster potx
... JHIII in any of the cell fractions. Transcriptional activation of DmEH with clofibrate Northern blot analysis revealed that transcription of DmEH in larvae was enhanced within 5 h of clofibrate ... Alignment of the five microsomal EH sequences. The deduced amino acid sequence of DmEH was compared with those of four other microsomal residential EHs. The abbreviations and accession n...
Ngày tải lên: 07/03/2014, 21:20
Báo cáo khoa học: Spectroscopic and kinetic properties of the horseradish peroxidase mutant T171S Evidence for selective effects on the reduced state of the enzyme potx
... Department of Biochemistry, School of Life Sciences, University of Sussex, Brighton, UK Horseradish peroxidase (HRPC) is a member of class III of the plant peroxidase superfamily and is cap- able of ... many of our ideas of biological oxidation reactions have been based [1]. More recently this has involved the detailed characterization of mutants [2–4] designed to probe va...
Ngày tải lên: 07/03/2014, 21:20
Báo cáo khoa học: Purification and properties of a new S-adenosyl-Lmethionine:flavonoid 4¢-O-methyltransferase from carnation (Dianthus caryophyllus L.) pot
... Double-reciprocal plot of inhibition kinetic of S-adenosyl- L - homocysteine (AdoHcy). Lineweaver–Burkplotof1/v vs. 1/[s] (where s ¼ kaempferol) in the presence of different fixed concentrations of S-adenosyl- L -methionine. ... the tissues of carnation (Dianthus caryophyllus L). The enzyme, with a molecular mass of 43–45 kDa and a pI of 4.15, specifically methylates the hydroxy su...
Ngày tải lên: 08/03/2014, 08:20
Báo cáo khoa học: On the aggregation properties of FMRP – a link with the FXTAS syndrome? pot
... being the presence of RGG motifs. FXR proteins are components of different types of nuclear and cytoplasmic ribonucleoprotein granules in which they often co-localize [1,15–19]. FXR proteins can form ... the nature of aggregation of FXR proteins, we have carried out a study of their aggregation properties using different approaches. We identified by in silico analysis potent...
Ngày tải lên: 14/03/2014, 23:20
Báo cáo khoa học: Human anionic trypsinogen Properties of autocatalytic activation and degradation and implications in pancreatic diseases potx
... expressed as percentage of trypsin activity measured at the beginning of the incubation. (B) Autolysis in the presence of 100 m M NaCl. (C) Effect of Ca 2+ ontherelativerateofautolysisinthe presence ... concentrations of Ca 2+ stabilized the enzyme, with an IC 50 of 5 l M (Fig. 6A,C). Addition of 100 m M NaCl to anionic trypsin decreased the rate of autolysis threefold (in the...
Ngày tải lên: 17/03/2014, 03:20
Báo cáo khoa học: "ON THE SUCCINCTNESS PROPERTIES OF UNORDERED CONTEXT-FREE GRAMMARS" ppt
... polynomiaUy bigger than the size of a grammar for the language itself. The proof of this claim rests on inspection of one of the standard proofs, say Hopcroft and Ullman [5]. The result is ... The number of strings in A is less than or equal to the number of ways of selecting one of the u strings. Consider the u string to the left of the boundary which z overlaps. Beca...
Ngày tải lên: 17/03/2014, 20:20