Báo cáo khoa học: Gc recruitment system incorporating a novel signal amplification circuit to screen transient protein-protein interactions pot

... primers 5¢-AAATA TAAAACGCTAGCGTCGACATGGCGC-3¢ and 5¢-AGC GTAAAGGATGGGGAAAG-3¢. The final ratio of target cells was determined by counting the number of colonies retaining the target genes. Acknowledgements This ... as expected. These data indicate that transient interactions cannot be isolated in a library- based screen with the Gc recruitment system. Thus, an advanced approach is...

Ngày tải lên: 05/03/2014, 23:20

... is the Storage Handler: Based on an automatic mime type 5 detection it decides how to store and retrieve documents. For example videos and audio material are best stored as files whereas XML documents ... tagger is a XML document it is stored as a XML Database. Finally the in- formation about the new document is stored in the Master Data including a reference to the original one in o...

Ngày tải lên: 31/03/2014, 20:20

... potentially involved (Riloff and Wiebe., 2003; Sarmento et al., 2009). Alternative approaches to automatic and manual construction of sentiment corpora have been pro- posed. For example, Kim and ... senti- ment annotated corpus. To measure the reliability of the sentiment anno- tations, we conducted an inter-annotator agreement trial, with two annotators. This was performed based on...

Ngày tải lên: 20/02/2014, 05:20

... small. Primers and linkers RT primer: 5¢-(biotin)ATTGGCGCGCCGCGAGCACTGAGTCAATACGA(T) 30 VN-3¢ rSAGER1: 5¢-GCGAGCACTGAGTCAATACGA-3¢ rSAGEF1: 5¢-AAGCAGTGGTATCAACGCAGAGT-3¢ TSP (tag-specific primer): ... oligo (dT) NVTTTTTTT NBAAAAAAA NVTTTTTTT NBAAAAAAA NVTTTTTTT NBAAAAAAA NVTTTTTTT NBAAAAAAA NVTTTTTTT 16 16 16 16 16 16 16 16 5′ 5′ 5′-cap oligo NVTTTTTTT NVTTTTTTT NBAAAAAAA NVTTTTTTT GGG CCC GGG...

Ngày tải lên: 07/03/2014, 00:20

... CA, USA), zinc sulfate (Caledon Laboratory Chemicals, Georgetown, Canada), ammonium formate buffer (Sigma-Aldrich, Oakville, Canada), ammonium hydroxide (BDH Chemi- cals ⁄ VWR, Mississauga, Canada), ... divided by a factor of 33. UV spectra were acquired using a Cary 5G UV-Vis-NIR spectrophotometer (Varian Canada, Mississauga, Canada) in a 1 cm quartz cuvette at room temperature (22 °C)...

Ngày tải lên: 16/03/2014, 06:20

... Ikeda 1 , Shinya Kajita 1 , Masaya Nakamura 2 and Yoshihiro Katayama 1 1 Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo, Japan; 2 Forestry ... °C, b-aryl ether cleavage activity was measured as described above. Localization of b-aryl ether cleavage activity A 14-day-old culture (4 mL) of 2BW-1 cells was separated i...

Ngày tải lên: 17/03/2014, 03:20

... solutions are compli- cated, consist of several stages and hand- built features, and are too slow to be applied as part of real applications that require such semantic labels, partly because of ... is labeled for each particular verb as so-called frames. Addition- ally, semantic roles can also be labeled with one of 13 ARGM adjunct labels, such as ARGM-LOC or ARGM-TMP for additional locatio...

Ngày tải lên: 17/03/2014, 04:20

... crucial limitation to the cytotoxicity of dimeric RNases. Abbreviations BS-RNase, bovine seminal RNase; ds-RNase A, domain-swapped RNase A; FAM-AUAA-TAMRA, 6-carboxyfluorescein-dArU(dA) 2 -6- carboxytetramethylrhodamine; ... be active [48–69%, relative to monomeric RNase A, with RNA as sub- strate; 1–5%, relative to monomeric RNase A, with 6-carboxyfluorescein-dArU(dA) 2 -6-carboxytet...

Ngày tải lên: 22/03/2014, 16:21

... 5¢-GTGTAGCGCGCGTGCG GCCC-3¢. The forward and reverse primers, respectively, for CaS were 5¢-AAATGGCAACGAAGTCTTCAC-3¢ and 5¢-CAGTCGGAGCTAGGAAGGAA-3¢. Isolation of plasma membrane, intact chloroplasts, stroma ... the thylakoid membrane of Arabidopsis thaliana Julia P. Vainonen 1 , Yumiko Sakuragi 2 , Simon Stael 1 , Mikko Tikkanen 1 , Yagut Allahverdiyeva 1 , Virpi Paakkarinen 1 , Eveliina Aro...

Ngày tải lên: 30/03/2014, 04:20

... 353 AACGACGATGAAAACGCCGGCGAGGTGGCGGATCGTGATCCGGTG CTTCTAGTATCTGGAATTGGAGGCTCTATTCTGCATTCTAAGAAGA AGAATTCAAAGTCTGAAATTCGGGTTTG TATATAGGTACCTTAACCAGAATCAACTACTTTGTG 354 ATATATGGATCCATGGGCTGGATTCCGTGTC ... H409L GGAGTGTCCTTCTATAACATA TTTGGAGTGTCACTTAATACACC Y346F GTCACTATCATCTCCCAT GCAATGGGAGGACTTATGGTTTC S17 7A CATATGTAGATGGAGCTGGAACTGTCCCTG D38 4A GGAGTGTCACTTAAT GCACCCTTTGATGTTTG T35 2A 37...

Ngày tải lên: 30/03/2014, 15:20