Tài liệu Báo cáo Y học: Control of p70 ribosomal protein S6 kinase and acetyl-CoA carboxylase by AMP-activated protein kinase and protein phosphatases in isolated hepatocytes pot
Tài liệu Báo cáo Y học: Control of p70 ribosomal protein S6 kinase and acetyl-CoA carboxylase by AMP-activated protein kinase and protein phosphatases in isolated hepatocytes pot
... Control of p70 ribosomal protein S6 kinase and acetyl-CoA carboxylase by AMP-activated protein kinase and protein phosphatases in isolated hepatocytes Ulrike Krause*, Luc Bertrand and Louis ... the inhibition of energy-consuming bio- synthetic pathways. Keywords:ACC;aminoacid;AMPK;p70S6K ;protein phosphatase. p70 ribosomal protein S6 kinas...
Ngày tải lên: 22/02/2014, 07:20
Tài liệu Báo cáo khoa học: Control of the coagulation system by serpins Getting by with a little help from glycosaminoglycans pptx
... Site-directed mutagenesis of arginine 103 and lysine 185 in the proposed glycosaminoglycan-binding site of heparin cofactor II. J Biol Chem 265, 286–291. Control of the coagulation system by serpins R. N. Pike ... Thrombomodulin enhances the reac- tivity of thrombin with protein C inhibitor by provid- ing both a binding site for the serpin and allosterically modulating t...
Ngày tải lên: 20/02/2014, 02:21
Tài liệu Báo cáo Y học: Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue pdf
... manganese. Selectivity of the proteins for their metal cofactor has been demonstrated in vivo [3] and although apoenzymes of each type of SOD may be reconstituted by the addition of metals, the resulting enzyme ... active site of the enzyme by reducing the occupied internal volume, allowing the intro- duction of an extra water molecule [29]. Replacement of the same resid...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: Inactivation of the 2-oxo acid dehydrogenase complexes upon generation of intrinsic radical species pptx
... 509–518. 66. Graceffa, P. (1983) Spin labeling of protein sulfhydryl groups by spin trapping a sulfur radical: application to bovine serum albu- min and myosin. Arch. Biochem. Biophys. 225, 802–808. 67. Gatti, ... signal between the protein and nonprotein fractions was investigated after precipitation of 2-oxo acid dehydrogenase complex by addition of 0.2 vol. of 35% polyet...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: Characterization of an omega-class glutathione S-transferase from Schistosoma mansoni with glutaredoxin-like dehydroascorbate reductase and thiol transferase activities pptx
... agarose. In all cases, protein purity was determined by SDS/PAGE and protein concentration was measured by bicinchoninic acid method following manu- facturers indications (Sigma). Antiserum against ... GSH. Ten microliters of the original protein, binding assay supernatant and eluate were analyzed by SDS/PAGE. Expression of SmGSTO along the parasite life cycle Total prote...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: Oxidation of phenols by laccase and laccase-mediator systems doc
... checked by 1 HNMR and HPLC. Spectrophotometric determination of laccase activity The activity of laccase was determined by following the rate of oxidation of 2,2¢-azinobis-(3-ethylbenzothiazoline-6- sulfonate) ... N-hydroxyphtalimide (HPI) and 2,2,6,6- tetramethylpiperidin-1-yloxy free radical (TEMPO) as mediators, since they greatly differ both in their mechan- ism of act...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: Characterization of a cloned subtilisin-like serine proteinase from a psychrotrophic Vibrio species doc
... N-terminal of the proteinase domain (see Fig. 2 or 3). The proteinase domain of VPR additionally contains Cys277 and Cys281, and Cys351 and Cys362 in the C-terminal domain, that suppo- sedly also ... enzymes displayed identical inactivation behaviour, indicating that the same disulfide(s) are involved in maintaining the integrity of active protein structure in both wild-typ...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: A novel meta-cleavage dioxygenase that cleaves a carboxyl-groupsubstituted 2-aminophenol Purification and characterization of 4-amino-3-hydroxybenzoate 2,3-dioxygenase from Bordetella sp. strain 10d doc
... an enzyme involved in the initial steps of the metabolism of 4-amino-3-hydroxybenzoic acid. 4-Amino-3-hydroxybenzoate 2,3-dioxygenase contained 1.9 mol Fe 2+ per mol of enzyme. Addition of Fe 2+ increased ... dioxygenase; 4-amino-3-hydroxybenzoate 2,3-dioxygenase. Dioxygenases catalyzing the fission of benzene rings are key enzymes in the microbial metabolic pathways of aromati...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: Inhibition of the MEK/ERK signaling pathway by the novel antimetastatic agent NAMI-A down regulates c-myc gene expression and endothelial cell proliferation ppt
... mediated, at least in part, by G -protein coupled receptors (GPCRs), stimulation of membrane phospholipid hydrolysis, and activation of the diacylglycerol sensitive isoforms of protein kinase C (PKC) [28]. ... as an internal control for mRNA integrity and equal loading. The levels of radioactivity incorporated into c-myc product were normalized by comparison with the...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: Role of electrostatics in the interaction between plastocyanin and photosystem I of the cyanobacterium Phormidium laminosum ppt
... copper protein plastocyanin can be replaced by cytochrome c 6 , a redox protein of similar size but entirely different folding, in a number of eukaryotic algae and cyanobacteria including P. laminosum ... sets of protein protein interactive surfaces with the same function as Cyt f – plastocyanin and plastocyanin–photosystem I – are available for identi- fication of featur...
Ngày tải lên: 21/02/2014, 01:21