Tài liệu Báo cáo Y học: Granule-bound starch synthase I A major enzyme involved in the biogenesis of B-crystallites in starch granules ppt

... the absence of concomitant amylopectin synthesis (reviewed in [41]). In vitro synthesis of amylose Because in Chlamydomonas amylose synthesis remains active when amylopectin synthesis has become minimal, wehavedecidedtoresorttoasemiin ... become minimal and it is difficult to say if the residual amylopectin synthesis activity is due to a residual soluble starch synthase act...

Ngày tải lên: 22/02/2014, 07:20

... factor Xa. TFPI has three Kunitz- type domains. The first domain binds to and inhibits factor VIIa, the second inhibits factor Xa. Inhibition of VIIa can proceed only after preliminary Xa binding. ... of the hypothetical reaction is defined by the correlation: ½VIIa À TF 0 / K XaÀVIIaÀTF D ¼ k XaÀVIIaÀTF d k VIIaÀTF;Xa a A2 2Þ In a similar fashion, the analysis of VIIa–TF...

Ngày tải lên: 22/02/2014, 04:20

... mobility is indica- ted by a line; the mobility of the Cyp4x1 specific band is indicated by an arrow. (C) Quantification of Cyp4x1 in brain microsomes. Thirty and 10 ng of purified recombinant Cyp4x1 ... brain; quantitation of the Cyp4x1 protein in brain demonstrated  10 ng of Cyp4x1 proteinÆmg )1 microsomal protein, showing that Cyp4x1 is a major brain P450. Immunohis...

Ngày tải lên: 19/02/2014, 07:20

... presence of either metal ion originates changes in the protein conformation, high affinity binding is achieved only in the presence of Mn 2+ . This high binding affinity of PEP in the presence of Mn 2+ appears ... characteristics of E. coli carb- oxykinase, very little information is available for ATP- dependent carboxykinases with respect to thermodynamic data on ligand b...

Ngày tải lên: 21/02/2014, 01:21

... of a second site. Also pointing in this direction is the second-order or biphasic shape of the inactivation curve of EII Glc by 1a (see residuals in Fig. 3). This may indicate that binding of a ... (a) EII Glc ,EII Man and the mannitol transporter, EII Mtl display biphasic phosphorylation kinetics towards their natural substrates, indicating that activity in vitro is...

Ngày tải lên: 21/02/2014, 01:21

... in Dnmt 3a, although this enzyme like Dnmt1 contains at least one additional DNA binding site in its N-terminal part [33]. The observation that Dnmt 3a is not activated by pre-exiting methylation ... to a second round of methylation with M.HpaII using radiolabeled AdoMet. No radioactivity was incorporated into the DNA demonstrating that all HpaII sites are fully methylated. RES...

Ngày tải lên: 21/02/2014, 01:21

... [33]. The fusion construct (pIM3982) was made by introducing an NsiI site at the translation start point of galA and by then cloning a fragment, starting at this NsiI site and containing the galA ... Methylation analysis demonstrated that a substantial amount of the L -arabinose residues (14%) in soy arabinogalactan is present as terminal residues [21], suggesting that of...

Ngày tải lên: 21/02/2014, 01:21

... Slower-migrating bands also visualized by SOD activity staining may have been derived from further interactions of the fusion protein GST domains (Fig. 2B). Although not visualized in these zymograms, ... opened the active site of the enzyme by reducing the occupied internal volume, allowing the intro- duction of an extra water molecule [29]. Replacement of the same re...

Ngày tải lên: 21/02/2014, 01:21

... missing in the latter [6]. As already mentioned, the missing galactose can be attributed to the phase variability of wlaN. The lack of terminal GalNAc in this strain has recently been shown by Gilbert ... similar in intensity to the intraresidue NOE 3D-5D, indicating that interproton pairs were in the 2–3 A ˚ range. The average distances obtained from molecular dyn...

Ngày tải lên: 21/02/2014, 01:21

... in the nacreous layer of the bivalve Pinctada maxima A new insight in the biomineralization field Lucilia Pereira-Mourie ` s 1 , Maria-Jose ´ Almeida 1,3 , Cristina Ribeiro 3 , Jean Peduzzi 2 , ... on individual proteins which enables the purification of different proteins in relative large amounts. In this work, we compare the nacre organic matrix obtained by the trad...

Ngày tải lên: 21/02/2014, 01:21