Tài liệu Báo cáo Y học: The solution structure and activation of visual arrestin studied by small-angle X-ray scattering pot

... Biochem. 269) 3809 The solution structure and activation of visual arrestin studied by small-angle X-ray scattering Brian H. Shilton 1 , J. Hugh McDowell 2 , W. Clay Smith 2 and Paul A. Hargrave 2,3 1 Department ... with phosphorylated R*. Keywords: visual arrestin; rhodopsin; G-protein coupled receptor signalling; small-angle X-ray scattering; solution...

Ngày tải lên: 22/02/2014, 07:20

... the presence of Glc slightly sensitized EII Glc for inactivation by the isothiocyanates 1e and 3d. On the other hand, the rate of inactivation by bromoacetyl-Glc (1a) was 15 times faster than by bromoacetic ... were recently introduced for the preparation of C-6 aldehyde derivatives of Glc [15]. The key step is the use of Collins reagent for the selective oxi...

Ngày tải lên: 21/02/2014, 01:21

... involves two key steps: branch migration, catalysed by the RuvB protein that is targeted to the Holliday junction by the structure specific RuvA protein, and resolution, which is catalysed by the RuvC ... highly structure specific in its binding to Holliday junctions. The effect of the charge on the central pin with respect to the specificity of the interaction...

Ngày tải lên: 21/02/2014, 01:21

... in the present structure, but they have a different orientation of t he hydroxyl group with respect to the X-ray structures. Side chain of Lys136 has different orientations in each of the X-ray ... Some of the H-bonds present in the human oxidized X-ray structures are observed also in the solution structure. The H-bonds among ring hydrogens of His43 and bac...

Ngày tải lên: 21/02/2014, 03:20

... 2 h and 20 h to determine the substrate specificity of the enzyme. The degradation of the polymer and subsequent oligosaccharide formation was analyzed by HPAEC and MALDI-TOF MS. HPAEC analysis ... HPAEC analysis of the hydrolysis of arabinogalactans by GALA. Three different arabinogalactans were used: potato (r), onion (j)andsoy (m). The release of monomers (A),...

Ngày tải lên: 21/02/2014, 01:21

... characterization of specific enzymes that are involved in the biosynthesis of these structures and may lead to the discovery of potential therapeutic targets. In addition, the correlation of carbohydrate structure ... regarding the sequences of sugar units was obtained by FAB-MS of the methylated core OS derivative. Figure 2 and Table 3 shows a series of A-type...

Ngày tải lên: 21/02/2014, 01:21

... Aiolos and Helios [6–8], and of Eos and Pegasus [9], revealed the existence of a family of related factors. Ikaros, Aiolos and Helios are all abundantly expressed in the haematopoietic system and ... shown in (B) and (C). The prominent bands seen above the bands of interest (arrowheads) cor- respond to the heavy chain of the antibodies used because of incom...

Ngày tải lên: 21/02/2014, 01:21

... related to the asymmetry of shape and possibility also to the size of the peptide molecule. A crude estimate of these two parameters, based simply on the structure and conformation of these peptides ... magnitude of their perturbation of phospholipid bilayer membranes and the growth of A. laidlawii, generally agrees well with the results of previous studies...

Ngày tải lên: 21/02/2014, 01:21

... very similar type of structure, possibly b ased on the PPII conformation, and which may b e envisaged as a more open version of the X -ray crystal structure of the Bowman±Birk inhibitor. The ... implying that they behave like synthetic high polymers in dilute aqueous solution for which the r andom coil model was originally developed. The random coil state is envisage...

Ngày tải lên: 21/02/2014, 15:20

... methods. Enzyme activity was a nalysed by the standard assay and the i mmunoreactivity was detected by Western blot analysis and quantitated as described i n Materials and methods. Open and filled ... located in the insert within the catalytic domain, close to the catalytic His264, and the proximity to the active site may explain the effect of oligomerization on...

Ngày tải lên: 21/02/2014, 15:20