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Tài liệu Báo cáo Y học: Differential effects of arachidonoyl trifluoromethyl ketone on arachidonic acid release and lipid mediator biosynthesis by human neutrophils pot

Tài liệu Báo cáo Y học: Differential effects of arachidonoyl trifluoromethyl ketone on arachidonic acid release and lipid mediator biosynthesis by human neutrophils pot

Tài liệu Báo cáo Y học: Differential effects of arachidonoyl trifluoromethyl ketone on arachidonic acid release and lipid mediator biosynthesis by human neutrophils pot

... AA-SF).3766 A. N. Fonteh (Eur. J. Biochem. 269) Ó FEBS 2002 Differential effects of arachidonoyl trifluoromethyl ketone on arachidonic acid release and lipid mediator biosynthesis by human neutrophils Evidence ... studies by demon-strating that inhibition of LTB4 and PAF biosynthesis by AACOCF3occurs without concomitant inhibition of AA release. Generation of the common precursor pool by CoA-IT occurs by ... biosynthesis viaacetylation of 1-alkyl-2-lyso-GPC by acetyl transferase(Fig. 8, f) activity, competition for 1-alkyl-2-lyso-GPCintermediate by various transferases may prevent reacyla-tion of AA. As...
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Tài liệu Báo cáo khoa học: Differential effects of Mxi1-SRa and Mxi1-SRb in Myc antagonism ppt

Tài liệu Báo cáo khoa học: Differential effects of Mxi1-SRa and Mxi1-SRb in Myc antagonism ppt

... that affect mycgene expression, Myc protein stability, and Myc bio-logical activity. Normal regulation of Myc activityoccurs by mechanisms that influence the Myc proteinper se [7], and also through ... converts Mxi1-SRa into apotent suppressor of Myc ⁄ Ras cotransformation activ-ity (Fig. 3) and also changes the activity of Mxi1-SRaactivity on the MYC promoter from activation torepression ... isoforms at the levels of Myc antagonism inthe REF assay, subcellular localization, and transcrip-tional activity. Some of these analyses have assigned differential functions to the two isoforms,...
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Tài liệu Báo cáo Y học: The effects of ring-size analogs of the antimicrobial peptide gramicidin S on phospholipid bilayer model membranes and on the growth of Acholeplasma laidlawii B ppt

Tài liệu Báo cáo Y học: The effects of ring-size analogs of the antimicrobial peptide gramicidin S on phospholipid bilayer model membranes and on the growth of Acholeplasma laidlawii B ppt

... phospholipid bilayer/water partition coefficient, the localization and orientationin the phospholipid bilayer, and the effects of the presence of these peptides on phospholipid organization and ... studies of GS/phospholipid bilayer interactions considerably.Specifically, we have investigated the strength and nature of the interactions of GS with phospholipid bilayers by utilizing differential ... of dimyristoylglycerophosphocholine (Myr2Gro-PCho), dimy-ristoylglycerophosphoethanolamine (Myr2Gro-PEtn) and dimyristoylglycerophosphoglycerol (Myr2Gro-PGro) by DSC, in order to determine their effects on phospholipidbilayer...
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Tài liệu Báo cáo Y học: The effects of low pH on the properties of protochlorophyllide oxidoreductase and the organization of prolamellar bodies of maize (Zea mays) pot

Tài liệu Báo cáo Y học: The effects of low pH on the properties of protochlorophyllide oxidoreductase and the organization of prolamellar bodies of maize (Zea mays) pot

... main line of evidence for the existence of a POR-phosphatase is the observation by Wiktorsson et al. [9] of aninhibition, by NaF and ATP, of the loss of the long-wavelength form of Chlide ... aggregated form of a phosphorylated (or possibly adenylated) ternary complex of POR, its substrate PChlide, and NADPH. Dephospho-rylation of the photoconverted form of this complex by anendogenous ... these conditions, hencethe lack of reformation of POR-PChlide650. Addition of lowconcentrations of NADPH to PLB aged at pH 7.5 whichshow only limited structural reorganization, in contrast,results...
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Tài liệu Báo cáo Y học: Differential response of neuronal cells to a fusion protein of ciliary neurotrophic factor/soluble CNTF-receptor and leukemia inhibitory factor pot

Tài liệu Báo cáo Y học: Differential response of neuronal cells to a fusion protein of ciliary neurotrophic factor/soluble CNTF-receptor and leukemia inhibitory factor pot

... differences.Neuronal differentiation of PC12 cells by Hyper-CNTFIn a second bioassay, we investigated the potential role of Hyper-CNTF in neuronal cell differentiation. Themorphology of rat pheochromocytoma ... 2002STAT3 and MAPK activation by Hyper-CNTFin transfected BAF/3 cellsDownstream signal transduction pathways were analyzed by studying the activation level of JAK/STAT and MAPkinase signaling components ... by activation of the MAPK pathway and that this response issubstantially independent of the JAK/STAT pathway.DISCUSSIONWe have successfully expressed an active fusion protein of human CNTF and human...
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Tài liệu Báo cáo khoa học: Structural effects of a dimer interface mutation on catalytic activity of triosephosphate isomerase The role of conserved residues and complementary mutations pptx

Tài liệu Báo cáo khoa học: Structural effects of a dimer interface mutation on catalytic activity of triosephosphate isomerase The role of conserved residues and complementary mutations pptx

... as a standard.Enzyme activityThe enzyme activity of TIM was determined by the conver-sion of glyceraldehyde 3-phosphate to dihydroxyacetonephosphate in the presence of TIM and a-glycerolphosphatedehydrogenase ... uncatalyzed rates of enolization of dihydroxyacetone phoshate and of glyceraldehyde 3-phosphate in neutral aqueoussolution. The quantitative assessment of the effective-ness ~of an enzyme catalyst. ... progress of the reaction for the triple mutant Y7 4W* is extremely slow, presumably because of theextremely low population of the catalytically competentdimeric species. Consequently, enzyme assays...
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Tài liệu Báo cáo khoa học: Inhibitory effects of nontoxic protein volvatoxin A1 on pore-forming cardiotoxic protein volvatoxin A2 by interaction with amphipathic a-helix doc

Tài liệu Báo cáo khoa học: Inhibitory effects of nontoxic protein volvatoxin A1 on pore-forming cardiotoxic protein volvatoxin A2 by interaction with amphipathic a-helix doc

... cells.Inhibitory effects of VVA1 on the hemolytic and cytotoxic activity of VVA2The effects of VVA1 on the hemolytic activity of VVA2 were studied by incubation of human red bloodcells (RBCs) with the ... Extendingthese results, we hypothesized that the inhibition of VVA2 cytotoxic pore formation by VVA1 can onlyoccur at the ideal ratio of 2 : 1 or lower, due to theability of one molecule of VVA1 to interact ... hemolytic and cyto-toxic activity of volvatoxin A2 (VVA2). (A) The hemolytic activity of VVA2 regulated by VVA1. VVA2 (45 nM) and various concentrations of VVA1 were preincubated as indicated, and...
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Tài liệu Báo cáo khoa học: Antimicrobial effects of H4-(86–100), histogranin and related compounds – possible involvement of DNA gyrase ppt

Tài liệu Báo cáo khoa học: Antimicrobial effects of H4-(86–100), histogranin and related compounds – possible involvement of DNA gyrase ppt

... devoid of resistance induction, due tothe evolutionary stability of histones, and more partic-ularly histone H4 [32]. Interestingly, mutations atcodon 751 of the E. coli gyrB gene conferred ... activity, as its replacement by Gly in HNb is most likely responsible for its relativelylow potency (LD50: 88.3 lgÆmL)1). Interestingly, theantimicrobial potency of HNb could be improved by amidation ... gyrase,are potentiated by ATP and modulated by chemicalagents that affect cell levels of ATP led us to verify the effects of HN and related compounds on the in vitrosupercoiling activity of...
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Tài liệu Báo cáo khoa học: Differential expression of duplicated LDH-A genes during temperature acclimation of weatherfish Misgurnus fossilis Functional consequences for the enzyme ppt

Tài liệu Báo cáo khoa học: Differential expression of duplicated LDH-A genes during temperature acclimation of weatherfish Misgurnus fossilis Functional consequences for the enzyme ppt

... properties (concentration and, as aconsequence, total activity) of an enzyme can be chan-ged by affecting rates of transcription and ⁄ or transla-tion and ⁄ or mRNA and protein degradation. Thisrepresents ... origin of highly homologous geneparalogs of skeletal LDH-A in the weatherfish.Many bony fish (Teleostei) are polyploidy, for exam-ple salmonids (Salmonidae) and cyprinids (Cyprinidae), and the ... UK); and clone managerprofessional suite (Scientific & Educational Software,Cary, NC, USA).Fragments of the fish LDH-A gene were isolated by means of reverse transcription followed by PCR....
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Tài liệu Báo cáo khoa học: Functional effects of deleting the coiled-coil motif in Escherichia coli elongation factor Ts pptx

Tài liệu Báo cáo khoa học: Functional effects of deleting the coiled-coil motif in Escherichia coli elongation factor Ts pptx

... translationindirectly by inhibition of mRNA synthesis during amino acid starvation [20–23].Kirromycin is one in a family of antibiotics that inhibitsprotein synthesis by binding EF-Tu–GTP and preventingthe ... translationin vitro was determined by poly(U)-directed polymerization of phenylalanine in the polymix system, as described by Ehrenberg et al. [42] and modified by Pedersen et al. [43]. Reaction mixtures ... Analysis of the elongation factor Tu (EF-Tu)–elongation factor Ts (EF-Ts) complex in the presence of guanine nucleotides and kirromycin by zone-interference gel electrophoresis. (A) Wild-type and...
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