Tài liệu Báo cáo Y học: Toxicity of substrate-bound amyloid peptides on vascular smooth muscle cells is enhanced by homocysteine docx

... inhibition of adhesion and subsequent toxicity to the cells by Ab may be important in cerebrovascular pathogenesis of amyloid angiopathy. The greater toxicity of the longer Ab1–42 species is consistent ... Toxicity of substrate-bound amyloid peptides on vascular smooth muscle cells is enhanced by homocysteine Su San Mok 1,2 , Bradley J. Turner 1,2...

Ngày tải lên: 22/02/2014, 07:20

... selectivity and specificity in the mononuclear SODs is not governed by one or even two residues, but is most likely accomplished by the concerted effects of a combination of key residues. Further analysis ... differen- tial effects of hydrogen peroxide on the FeSOD mutants may be explained by an alteration of reactivity induced by the electronic configuration of active s...

Ngày tải lên: 21/02/2014, 01:21

... Journal compilation ª 2007 FEBS 6271 dimethylation of Arg, monomethylation, dimethylation and trimethylation of Lys, acetylation and ubiquitina- tion of Lys, and phosphorylation of Ser and Thr ... 1. Fragmentation scheme for production of ions of type c¢ and z¢ Æ by reaction of a low-energy electron with a multiply protonated peptide. ETD-MS analysis of peptides and protei...

Ngày tải lên: 18/02/2014, 16:20

... inhibition of tRNA Gly 1 -1 by the compet- ing tRNA Gly 1 -6,7 (lane 3), was rescued very efficiently by increasing concentrations of TFIIIB (lanes 5 and 6) but not by pol III (lane 4). Transcription ... in the sequestration of TFIIIB presumably by binding to the TBP component of TFIIIB. This inference was further confirmed by immuno- depletion of TFIIIB using a polyclonal a...

Ngày tải lên: 20/02/2014, 02:21

... substrate specificity of the enzyme. The degradation of the polymer and subsequent oligosaccharide formation was analyzed by HPAEC and MALDI-TOF MS. HPAEC analysis demon- strated that already after 15 ... and methods. Hydrolysis of arabinogalactans The sugar composition of the arabinogalactans from potato, onion and soy was determined as described [21]. Onion arabinogalactan consists...

Ngày tải lên: 21/02/2014, 01:21

... complex-catalyzed succinyl-CoA hydro- lysis [72] accounts for the inactivation by dihydrolipoamide in the presence of succinyl-CoA (Table 2). DISCUSSION Generation of radical species during catalysis by ... backward reaction of NADH oxidation [22]. Under physiological conditions, this reaction is unlikely to contribute to the mitochondrial metabolism significantly: NADH is a stro...

Ngày tải lên: 21/02/2014, 01:21

... Purification yield approximately 500 lg of protein per milliliter of S-hexyl glutathione agarose. In all cases, protein purity was determined by SDS/PAGE and protein concentration was measured by bicinchoninic ... for SmGSTO and the strong inhibitory effect of S-hexyl glutathione on the enzyme activity. The preference of SmGSTO for more hydrophobic alkyl-bound S-hexyl glutathione...

Ngày tải lên: 21/02/2014, 01:21

... Poliporus pinsitus was kindly donated by Novo Nordisk Biotech and purified by ion-exchange chromatography on Q-Sepharose by elution with phosphate buffer [5,20], and an activity of 10 000 UÆmL )1 determined spectrophotometrically ... determination of laccase activity The activity of laccase was determined by following the rate of oxidation of 2,2¢-azinobis-(3-ethylbenzothia...

Ngày tải lên: 21/02/2014, 01:21

... proteinases of this comparative study, we also determined the denaturation curves of the enzymes inhibited by phenylmethanesulfonyl fluoride, as a function of denat- urant concentration. Several ... loops of the C- and N-termini of the protein, calcium is coordinated by the carboxylate oxygens of Asp260 and the backbone oxygen of Thr16, in addition to 4–5 water molecules [5...

Ngày tải lên: 21/02/2014, 01:21

... Ventura 1,2 1 Department of Biomedical Sciences, Division of Biochemistry, Laboratory of Cardiovascular Research, 2 Division of Cell Biology, National Institute of Biostructures and Biosystems, and 3 Department of ... activation is mediated, at least in part, by G-protein coupled receptors (GPCRs), stimulation of membrane phospholipid hydrolysis, and activation of the diac...

Ngày tải lên: 21/02/2014, 01:21