Tài liệu Báo cáo Y học: Phosphorylation of initiation factor-2a is required for activation of internal translation initiation during cell differentiation ppt

... cells. Mol. Cell 6, 1099–1108. Ó FEBS 2002 eIF2a phosphorylation and IRES activity (Eur. J. Biochem. 269) 2819 Phosphorylation of initiation factor-2a is required for activation of internal translation ... Increased phosphorylation of eukaryotic initiation factor 2 alpha at the G2/M boundary in human osteosarcoma cells correlates with deglycosylation of p67 a...

Ngày tải lên: 22/02/2014, 07:20

... specificities of isolated antisera were tested by Western analysis. RNA isolation and Northern-analysis Total R NA was isolated by guanidinium thiocyanate extraction [25] from embryos, pupae, larvae, ... ability of CK2 a subunit to phosphorylate calmodulin and this inhibition can be overcome by the polylysine It was shown that in contrast to the stimulatory effect on phosphorylation of...

Ngày tải lên: 21/02/2014, 15:20

... analysis of the entire set of chimeras prepared in this study, which allowed us to assay directly the condensation activity of the isolated (His) 6 -FAE1 KCSs [20]. We used this assay to verify ... usefulness of this in vivo system as a quick screen for activities of FAE1 KCSs. However, the results are reflective of the entire yeast metabolic pathways from the synthesis of 3-ke...

Ngày tải lên: 21/02/2014, 15:20

... comparison with previously studied cytochrome c oxidases is presented and on this basis the contributions of the reorganization of the polypeptide backbone, of individual amino acids and of the hemes ... centers discussed. Keywords: caa 3 oxidase; cytochrome c oxidase; UV/VIS- spectroscopy; FT-IR-spectroscopy; Thermus thermophilus. Cytochrome c oxidase is the terminal enzyme of...

Ngày tải lên: 21/02/2014, 15:20

... ber of words in keyword dictionary was about 800. In his method, duration of discourses of switch board corpora is rather long and there are many keywords in the discourse. However, for a ... manually and calcu- lated 5 feature vectors for classifying domains of each unit of radio news and for extracting key- words. Our previous system could not extract some keywords...

Ngày tải lên: 20/02/2014, 18:20

... asynchronously growing cells (asynchr.) was also analyzed. The percentage of cells in the G0/G1, S, and G2/M phases of the cell cycle at each time point was determined by FACS analysis and is shown ... address this possibility it will be necessary to generate a higher affinity antiserum that is specific for the E2F6 protein. Translation of E2F6b is initiated at the internal...

Ngày tải lên: 08/03/2014, 09:20

... secretion is the key s tep in synaptic transmission and is the basis of intercellular communication in the nervous system. Synaptic vesicle exocytosis is regulated by Ca 2+ and by a large number of ... example, the phosphorylation of synaptic vesicle-associated protein SV2 by CKI modulates its ability to interact with synap- totagmin. Phosphorylation of syntaxin-1A by CKII i...

Ngày tải lên: 22/02/2014, 04:20

... herefore, a study on the cell cycle-dependent regulation of the interaction of LBR and chromatin is important for elucidation of the nuclear envelope assembly/disassembly mechanism. In this stud ... differentiated somatic cell divisions. Thus, the structure of nuclear envelopes changes very dynamically d epending on the stage o f the cell cycle. T o ensure the p recise assem...

Ngày tải lên: 22/02/2014, 04:20

... performance of the assay was reliable only within a limited range of probe concentrations), dialysis after phosphorylation was not necessary, probably because of a lower degree of interaction of ... protein perilipin, are also of key importance. In adipocytes, stimulation of lipolysis by catecholamines results in activation of adenylate cyclase, leading to elevated Keyword...

Ngày tải lên: 18/02/2014, 11:20

... activity is controlled is phosphorylation at tyrosine 17 (Y1 7) of CDK4. This site corresponds to tyrosine 15 (Y1 5) of CDK1 (Cdc2), a site of inhibitory phosphorylation on this kinase [10,11]. Phosphorylation ... cancer. The activity of cyclin dependent kinase 4 is controlled by multiple mechanisms, including phosphorylation of tyrosine 17. This site is equivalent t...

Ngày tải lên: 18/02/2014, 18:20