Tài liệu Báo cáo Y học: EPR characterization of the mononucl

Tài liệu Báo cáo Y học: EPR characterization of the mononuclear Cu-containing Aspergillus japonicus quercetin 2,3-dioxygenase reveals dramatic changes upon anaerobic binding of substrates potx

... EPR characterization of the mononuclear Cu-containing Aspergillus japonicus quercetin 2,3-dioxygenase reveals dramatic changes upon anaerobic binding of substrates Ingeborg ... perturbation of the axial symmetry of the tensor, whereas axial symmetry, i.e. g xx % g yy << g zz is typically found for type 2 sites. The grouping of the g-valu...

Ngày tải lên : 22/02/2014, 07:20

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Tài liệu Báo cáo khoa học: Functional characterization of an orphan cupin protein from Burkholderia xenovorans reveals a mononuclear nonheme Fe2+-dependent oxygenase that cleaves b-diketones ppt

... indirectly by measuring the increase in absorbance at 550 nm resulting from cyto- chrome c being reduced by superoxide in the course of the reaction. The principle of the assay is that the presence of ... comparison of nonheme metal- dependent enzymes. Fig. S2. Overlay of the homology model of Bxe_A2876 (white) with the template crystal structure of RgCarb (RgCAR...

Ngày tải lên : 18/02/2014, 06:20

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Tài liệu Báo cáo khoa học: Kinetic characterization of the ﬁrst step of the ribozyme-catalyzed trans excision-splicing reaction docx

... seen with other ribozymes [6,8,13,18,19,43]. Thus, the rate of assembly of the Pneumocystis ribozyme– substrate complex appears to be limited by the process of helix formation. Nevertheless, because ... Tetrahymena there is only a twofold difference. Apparently, there are additional or more stable interactions that the ribozyme uses to bind the product relative to the ribozy...

Ngày tải lên : 18/02/2014, 18:20

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Tài liệu Báo cáo Y học: Molecular modeling of the dimeric structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain docx

... as the high- afﬁnity peak of wild-type LPL. The LPL in the eluate was detected by Western blot with LPL polyclonal antibody 7640. The fraction number of the high-afﬁnity peak was the same for the ... recognition site in the C-terminal domain of the other face each other, thereby bringing together the substrate -binding and catalytic functions in proximity for effect...

Ngày tải lên : 21/02/2014, 15:20

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Tài liệu Báo cáo Y học: Structural determinants of the half-life and cleavage site preference in the autolytic inactivation of chymotrypsin pdf

... used. RESULTS The enzymatic activity and molecular stability of the mutants The catalytic activity and the molecular stability of the mutants were assayed because a change in either of these parameters ... Thus, it is the cleavage of the interdomain loop and not of the autolysis or other loops that determines the half-life of chymotrypsin activity. Keywords: auto...

Ngày tải lên : 22/02/2014, 07:20

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Tài liệu Báo cáo Y học: Solution structure of the mEGF/TGFa44250 chimeric growth factor doc

... 2001 geometry, the set of solutions reflects any uncertainty in the calibration as well as alternative fits to the set of distances. The main advantages of the procedure are simple: the details of the ... by modification of the standard cysteine residue in the DYANA library [53]. A pseudoatom was included instead of the HG atom and a bridge was formed by superimpo...

Ngày tải lên : 22/02/2014, 07:20

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Tài liệu Báo cáo khoa học: Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y•) involved in ribonucleotide reduction ppt

... to the catalytic site of the R1E subunit. As reported in the Results, upon completion of substrate conversion, the radical is then rapidly passed back from the R1E subunit to the tyrosyl of the ... affected by the addition of HU. The tyrosyl radical completely decayed within 10 s of substrate addition. The degree of tyrosyl radical loss was dependent on...

Ngày tải lên : 15/02/2014, 01:20

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Tài liệu Báo cáo khoa học: Molecular characterization of Arabidopsis thaliana PUF proteins – binding speciﬁcity and target candidates doc

... was fully abolished (Fig. 3D). Furthermore, assays using APUM-7 as prey did not interact with the wild-type or any of the mutant NREs (Fig. 3C). To conﬁrm that the result of binding speciﬁcity observed ... each APUM in the PFAM analysis. Gray circles represent the localization of repeats in the protein and the numbers indicate the position of each repeat in the PUF...

Ngày tải lên : 18/02/2014, 06:20

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Tài liệu Báo cáo khoa học: Spectroscopic characterization of a higher plant heme oxygenase isoform-1 from Glycine max (soybean) ) coordination structure of the heme complex and catabolism of heme docx

... some of the details are still unknown, the degradation of heme by HO follows the mechanism shown in Scheme 1. Heme catabolism by mammalian HO proceeds sequentially by way of oxyheme, hydroxyheme, ... estimate the yield of free biliverdin produced by the GmHO-1 reaction, the bilirubin assay was carried out by use of rat BVR. The overall yields of bilirubin after the...

Ngày tải lên : 19/02/2014, 05:20

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Tài liệu Báo cáo khoa học: Thermodynamic characterization of interleukin-8 monomer binding to CXCR1 receptor N-terminal domain ppt

... enthalpy of binding is linearly related to the intrinsic ionization enthalpy of the buffer. The relationship between the ionization enthalpy of the buffer and the measured enthalpy is given by the ... determine the enthalpy (DH), entropy (DS), and the free energy (DG) of binding of monomeric IL-8 to the receptor CXCR1 N-domain. The binding isotherm of I...

Ngày tải lên : 19/02/2014, 05:20

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