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Tài liệu Báo cáo Y học: Importance of the amino-acid composition of the shutter region of plasminogen activator inhibitor-1 for its transitions to latent and substrate forms pdf

Tài liệu Báo cáo Y học: Importance of the amino-acid composition of the shutter region of plasminogen activator inhibitor-1 for its transitions to latent and substrate forms pdf

Tài liệu Báo cáo Y học: Importance of the amino-acid composition of the shutter region of plasminogen activator inhibitor-1 for its transitions to latent and substrate forms pdf
... Both transitions to latent and substrate forms were strongly but differently influenced by the amino-acid composition of the shutter region. Surprisingly, we found that substitution of Lys335 to ... to as the shutter region [2]. By high resolution X-ray crystal structure analysis of the native form of the serpin plasminogen activator inhibitor-2...
Ngày tải lên : 22/02/2014, 07:20
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Tài liệu Báo cáo Y học: Dnmt3a and Dnmt1 functionally cooperate during de novo methylation of DNA pdf

Tài liệu Báo cáo Y học: Dnmt3a and Dnmt1 functionally cooperate during de novo methylation of DNA pdf
... because methylation by Dnmt3a is the initial event for de novo methylation and due to its own de novo activity Dnmt1 might be able to stimulate itself in an autocatalytic fashion, iftoomuchofthisenzymeisused. We ... development and even in adult cells to silence acquired proviral DNA or to alter the developmental program of the cell. Aberrant de novo methylation may le...
Ngày tải lên : 21/02/2014, 01:21
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Tài liệu Báo cáo Y học: Soluble silk-like organic matrix in the nacreous layer of the bivalve Pinctada maxima A new insight in the biomineralization field pptx

Tài liệu Báo cáo Y học: Soluble silk-like organic matrix in the nacreous layer of the bivalve Pinctada maxima A new insight in the biomineralization field pptx
... previously [20]. The serine, threonine and tyrosine contents of the hydrolysates were corrected for destruction during the hydrolysis by extrapolation to zero time hydro- lysis. The amino acid compositions, ... very similar to the WSM, and consequently the EDTA-IM. Hydroxyproline, hydroxy- lysine and phosphoserine were not detected in WSM, WIM, EDTA-SM and EDTA-IM....
Ngày tải lên : 21/02/2014, 01:21
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Tài liệu Báo cáo Y học: BIGH3 (TGFBI) Arg124 mutations influence the amyloid conversion of related peptides in vitro Implications in the BIGH3-linked corneal dystrophies pptx

Tài liệu Báo cáo Y học: BIGH3 (TGFBI) Arg124 mutations influence the amyloid conversion of related peptides in vitro Implications in the BIGH3-linked corneal dystrophies pptx
... decades of life and severely impair visual acuity in adulthood. Their therapy is restricted to keratopl- asty and phototherapeutic keratectomy by Excimer laser. Unfortunately, the benefits of these therapies ... degree of amyloid fibril formation. Cysteine has the capacity to form disulfide bonds. As disulfide bonding of the protein may participate in the appearance of a...
Ngày tải lên : 21/02/2014, 01:21
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Tài liệu Báo cáo Y học: A novel, inducible, citral lyase purified from spores of Penicillium digitatum docx

Tài liệu Báo cáo Y học: A novel, inducible, citral lyase purified from spores of Penicillium digitatum docx
... liquid samplesweretakenandanalysedbyGCandGC-MSto determine the products formed. Electrophoresis SDS/PAGE was used to assess purity of enzyme prepara- tions and determine the molecular mass of the purified enzyme ... as the standard. RESULTS Induction of citral lyase Initially, the reproducibility of the results was hindered by variations in the citral lyase activity...
Ngày tải lên : 21/02/2014, 01:21
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Tài liệu Báo cáo Y học: Ligand interactions and protein conformational changes of phosphopyridoxyl-labeled Escherichia coli phosphoenol pyruvate carboxykinase determined by fluorescence spectroscopy pdf

Tài liệu Báo cáo Y học: Ligand interactions and protein conformational changes of phosphopyridoxyl-labeled Escherichia coli phosphoenol pyruvate carboxykinase determined by fluorescence spectroscopy pdf
... The phosphoryl and pyridoxyl moieties of the P-pyridoxyl group are shown in red and magenta, respectively. The fractional solvent exposed area of the P-pyridoxyl group is 0.39 and 0.082 for the ... 2002 conformational changes caused by the ligand are easily sensed by the P-pyridoxyl chromophore. Figure 4 shows the modeled structure of the P-pyridoxyl-PEP carbox...
Ngày tải lên : 21/02/2014, 01:21
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Tài liệu Báo cáo Y học: The glucose-specific carrier of the Escherichia coli phosphotransferase system Synthesis of selective inhibitors and inactivation studies pptx

Tài liệu Báo cáo Y học: The glucose-specific carrier of the Escherichia coli phosphotransferase system Synthesis of selective inhibitors and inactivation studies pptx
... were recently introduced for the preparation of C-6 aldehyde derivatives of Glc [15]. The key step is the use of Collins reagent for the selective oxidation of the primary trimethyl- silyl ether of the ... G. (1986) The oxidation of primary trimethylsilyl ethers to aldehydes: a selective conversion of a primary hydroxy group into an aldehyde group in the pr...
Ngày tải lên : 21/02/2014, 01:21
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Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf

Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf
... 2 h and 20 h to determine the substrate specificity of the enzyme. The degradation of the polymer and subsequent oligosaccharide formation was analyzed by HPAEC and MALDI-TOF MS. HPAEC analysis ... producing transformant and was used for the purification of GALA. Growth of this transformant and subsequent purification of the enzyme were performed as describe...
Ngày tải lên : 21/02/2014, 01:21
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Tài liệu Báo cáo Y học: Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue pdf

Tài liệu Báo cáo Y học: Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue pdf
... above. Assay for superoxide dismutase activity The specific activity of SOD was measured spectrophoto- metrically by its inhibitory action on the superoxide- dependent reduction of cytochrome c by xanthine-xanthine oxidase ... conclusive result. Effect of hydrogen peroxide on enzyme activity Hydrogen peroxide, the product of the SOD reaction, is an inhibitor of SODs and...
Ngày tải lên : 21/02/2014, 01:21
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Tài liệu Báo cáo Y học: The structures of the lipooligosaccharide and capsule polysaccharide of Campylobacter jejuni genome sequenced strain NCTC 11168 pdf

Tài liệu Báo cáo Y học: The structures of the lipooligosaccharide and capsule polysaccharide of Campylobacter jejuni genome sequenced strain NCTC 11168 pdf
... was restricted to the )60° conformer for the DL form of residue D andto+60° for the LD form. For the trisaccharide, 5 · 10 3 macro moves were used with a step length of 10° for the glycosidic linkage and ... Subsequent 1D-NOESY-TOCSY (H-8D, 200 ms; H-6D, 60 ms) for detection of the H-7D and H-7¢D resonances. (d) 1D-TOCSY-NOESY (H-4D H-8D, 30 ms; H-5D, 200 ms)...
Ngày tải lên : 21/02/2014, 01:21
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