Tài liệu Báo cáo Y học: Re-oxygenation of hypoxic simian virus 40 (SV40)-infected CV1 cells causes distinct changes of SV40 minichromosome-associated replication proteins doc
Tài liệu Báo cáo Y học: Granule-bound starch synthase I A major enzyme involved in the biogenesis of B-crystallites in starch granules ppt
... submitted to in vitro synthesis. Time of incubation (h) a Total starch (mg) % Amylopectin % Amylose % of A-type crystals (± 5%) % of B-type crystals (± 5%) % of total crystallinity (± 3%) 0 13.9 87 ... displays a high crystallinity of the A-type with few B-type crystals present. After a mere 24 h, crystallinity has decreased from 42 to 32% whilst B-type crystals have increased from...
Ngày tải lên: 22/02/2014, 07:20
Tài liệu Báo cáo Y học: Ligand interactions and protein conformational changes of phosphopyridoxyl-labeled Escherichia coli phosphoenol pyruvate carboxykinase determined by fluorescence spectroscopy pdf
... phosphoenolpyruvate (PEP) carboxykinase catalyzes the decarboxylation of oxaloacetate and transfer of the c-phosphoryl group of ATP to yield PEP, ADP, and CO 2 . The interaction of the enzyme with the substrates ... pyridoxamine, with a maximum at 393 nm. This spectral behavior reflects a high degree of exposure of the P-pyridoxyl group to the solvent. The fluorescence decay of py...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: The glucose-specific carrier of the Escherichia coli phosphotransferase system Synthesis of selective inhibitors and inactivation studies pptx
... stereoselective synthesis of enantiomerically pure mono- and di-O-hexadecyl-b- D -glucosylglycerol ethers by epoxidation of an allyl b- D -glucopyranoside asymmetrically induced by the glucide moiety. Tetrahedron: ... The oxidation of primary trimethylsilyl ethers to aldehydes: a selective conversion of a primary hydroxy group into an aldehyde group in the presence of a secondary hy...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: Dnmt3a and Dnmt1 functionally cooperate during de novo methylation of DNA pdf
... groups are always introduced, one slowly by Dnmt3a or by de novo methylation catalyzed by Dnmt1 and the second one very fast. Therefore, this effect can only double the rate of DNA methylation, because every ... novo methylation of DNA [15]. Interestingly, the de novo activity of Dnmt1 is stimulated by binding of methylated DNA to an allosteric site located in the N-terminal domain o...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf
... substrate specificity of the enzyme. The degradation of the polymer and subsequent oligosaccharide formation was analyzed by HPAEC and MALDI-TOF MS. HPAEC analysis demon- strated that already after 15 ... N-glycosylation site. BLAST analysis of the deduced amino acid sequence of GALA revealed very high similarity to the endogalactanases of A. tubingensis (95.7% amino acid sequence id...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue pdf
... manganese. Selectivity of the proteins for their metal cofactor has been demonstrated in vivo [3] and although apoenzymes of each type of SOD may be reconstituted by the addition of metals, the resulting enzyme ... apparently the least sensitive of all SODs tested (K i approx. 40 m M , results not shown and Fig. 5). Effect of temperature on enzyme activity The thermostability...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: The structures of the lipooligosaccharide and capsule polysaccharide of Campylobacter jejuni genome sequenced strain NCTC 11168 pdf
... the detection of NOEs for H-5D. (e) 1D-TOCSY-NOESY (H-5D, 40 ms; H-4D, 400 ms) for detection of the NOEs for H-4D. (f) 1D-TOCSY-NOESY (H-2D, 40 ms; H-3D, 400 ms) for detection of NOEs for H-3D. ... A.M., Wu, Y. , Young, N.M. & Wakarchuk, W.W. (2000) Biosynthesis of ganglioside mimics in Campylobacter jejuni OH4384. Identification of the glycosyltransferase genes, enzymatic...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: Soluble silk-like organic matrix in the nacreous layer of the bivalve Pinctada maxima A new insight in the biomineralization field pptx
... o-phthaldial- dehyde derivatives of amino acids were detected with a Waters 420 fluorimeter. Proline, hydroxyproline and hydroxylysine were examined at 254 nm by reverse-phase HPLC of their phenylisothiocarbamate ... as reported previously [20]. The serine, threonine and tyrosine contents of the hydrolysates were corrected for destruction during the hydrolysis by extrapolation to zero ti...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: Inactivation of the 2-oxo acid dehydrogenase complexes upon generation of intrinsic radical species pptx
... Site-directed mutagenesis of dihy- drolipoamide acetyltransferase and post-translational modifica- tion of its lipoyl domains. In Biochemistry and Physiology of Thiamin Diphosphate Enzymes (Bisswanger, ... Biochem. Biophys. 246, 501–514. 64. Ambrose, M.C. & Perham, R.N. (1976) Spin-label study of the mobility of enzyme-bound lipoic acid in the pyruvate dehy- drogenase multienzyme...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: BIGH3 (TGFBI) Arg124 mutations influence the amyloid conversion of related peptides in vitro Implications in the BIGH3-linked corneal dystrophies pptx
... [12] in a phenotypic variant of granular corneal dystrophy type 1 (GCD1), Arg124His (R124H) [1] in granular corneal dystrophy type 2 (GCD2, Avellino dystrophy) a mixed type of amyloid and granular ... diseases. Keywords: amyloidosis; keratoepithelin; lattice corneal dys- trophy; granular corneal dystrophy; synthetic peptide. Hereditary corneal dystrophies are a cause of blindness. These...
Ngày tải lên: 21/02/2014, 01:21