Tài liệu Báo cáo Y học: Exploring the role of a glycine cluster in cold adaptation of an alkaline phosphatase pdf

... 2002 Exploring the role of a glycine cluster in cold adaptation of an alkaline phosphatase Konstantinos Mavromatis 1, *, Iason Tsigos 2, *, Maria Tzanodaskalaki 2 , Michael Kokkinidis 1,3 and Vassilis ... the active site of the psychrophilic alkaline phosphatase from the Antarctic Table 1. Enzymatic and thermodynamic parameters of the psychrophilic...

Ngày tải lên: 22/02/2014, 04:20

... form the ba- sis for assigning a given text to a certain genre are reflected in the style of the text, and if that style can be characterised quantitatively as a ten- dency to favour certain ... marks for CL. We then determined the relevance of each of these lemmata for a given classifica- tion task by their gain ratio (Yang and Pedersen, 1997). From this ranked...

Ngày tải lên: 22/02/2014, 03:20

... solvents in enzymatically cata- lyzed reactions has gained increasing importance [1,2]. Unfortunately, most of these solvents act as a denaturant. Like conventional denaturants such as guanidine hydro- chloride ... information on conformational changes of RNase A in trifluoroethanol. In the absence of trifluoroeth- anol, subtilisin Carlsberg and proteinase K degrade RNa- se A...

Ngày tải lên: 22/02/2014, 07:20

... has shown that k XaÀVIIaÀTF ;TFPI a and k TFPIÀXaÀVIIaÀTF d are practically independent of k VIIaÀTF ;Xa a when the latter is changed in the range of 0–10 n M )1 Æmin )1 , and change of k XaÀVIIaÀTF d does ... plasma and binds to circulating factor VIIa, greatly enhancing its proteolytic activity. The VIIa–TF complex activates factors IX and X via limited proteolysis. This i...

Ngày tải lên: 22/02/2014, 04:20

... esters of methyl a- D -glucopyranoside ( 1a) andmethyla- D -manno- pyranoside (1c), methyl 6-deoxy-6-isothiocyanato -a- D - glucopyranoside (1e), b- D -glucopyranosyl isothiocyanate (3c)andb- D -glucopyranosyl ... mutually interacting binding sites containing an active-site cysteine that is accessible from both faces of the membrane. The nature of the structural rearrangement...

Ngày tải lên: 21/02/2014, 01:21

... detected against any of the transgalactooligosaccharides listed in Materials and methods. Hydrolysis of arabinogalactans The sugar composition of the arabinogalactans from potato, onion and soy was ... 6.6% L -arabinose, while soy arabinogalactan consists of 57% D -galactose and 38% L -arabinose. Methylation analysis demonstrated that a substantial amount of the L -arabi...

Ngày tải lên: 21/02/2014, 01:21

... mutants) and per-manganese metal basis (manganese superoxide dismutase mutants). Table 2. Metal contents of mutant superoxide dismutases. Metal con- tent was measured by atomic absorbance and ... [11,22,23]. Beyond the metal ligand residues and within 10 A ˚ of the metal there are few significant differences between iron- and manganese-containing SODs. During catalysis substrate and...

Ngày tải lên: 21/02/2014, 01:21

... the terminal galactose and GalNAc are missing in the latter [6]. As already mentioned, the missing galactose can be attributed to the phase variability of wlaN. The lack of terminal GalNAc in this strain ... followed by a final annealing at 72 °C for 10 min using primers: Cj1427cF918 (5¢-AACTTTCATCATTTTAAAC GCTCTT-3¢) and Cj1427cR10 (5¢-AAAGTTTTAATTAC AGGTGGTGCAG-3¢). Deoxy...

Ngày tải lên: 21/02/2014, 01:21

... WSM. Glycosaminoglycan analysis Glycosaminoglycans are highly negatively charged because of the presence of sulfate ester and/or carboxyl groups. Therefore, they interact with cations and are precipitated ... Sulfated and nonsulfated glycosamino- glycans (GAGs) from the supernatant were estimated by the Whiteman Alcian blue binding technique [28,29], using chondroitin sulfate as sta...

Ngày tải lên: 21/02/2014, 01:21

... developed after the delay. The delayed signal was inhibited by concomitant addition of SOD and the metal chelator DTPA. These data show that initial PBN adducts are dependent on the superoxide anion radical ... In partic- ular, the appearance of the ThDP adduct with the substrate moiety during the complex-catalyzed succinyl-CoA hydro- lysis [72] accounts for the inacti...

Ngày tải lên: 21/02/2014, 01:21