... overNi-nitrilotriacetic acid resin, according to the instructions of the manufacturer. A single protein band was detected by SDS/PAGE of purified enzymes.Enzyme activities were determined by measuring the formation ... 5¢-ATTAATGGCATGCTTTACCCGT-3¢.UsingthePCRproducts of agmatinase with the GfiAandCfiT substi-tutions in the coding and noncoding strands, respectively, and using the 5¢ and 3¢ primers mentioned above, the ... de Concepcio´n, Casilla 160 -C, Concepcio´n,Chile;2Center for Genetic Engineering and Biotechnology, Habana, CubaUpon mutation of Asp153 by asparagine, the catalyticactivity of agmatinase...