Tài liệu Báo cáo Y học: Phosphatidylinositol synthesis and exchange of the inositol head are catalysed by the single phosphatidylinositol synthase 1 from Arabidopsis docx

... APDyTTPEMY-NH 2 were obtained from Dr A ˚ ke Engstro ¨ m at the Peptide Synthesis and Analysis Laboratory of the Department of Medical Biochemistry and Microbio- logy, Uppsala University. The ... months. The phosphate content of the phosphopeptide was analysed by the malachite green method [19 ]. The peptide concentration was calculated by using the molar abs...

Ngày tải lên: 21/02/2014, 01:21

... TC105068 1 1 1 3 AtPCa TC1 212 67 2 2 AtP32 TC 117 759 1 12 AtP 31 TC123560 1 1 2 AtP14 TC 111 256 22 AtP46 TC 112 253 1 1 AtP30 – 11 AtP18 TC 116 977 11 AtP36 TC122998 1 1 AtP25 TC 117 275 11 AtP28 TC105 819 ... TC 115 545 17 218 37 AtP 11 TC109595 24 16 31 AtP4 TC103 612 1 1 4 6 5 9 1 27 AtP19 TC 115 663 15 2 7 24 AtP17 TC1 215 24 2 2 13 5 22 AtP15 TC109700 8 1...

Ngày tải lên: 21/02/2014, 01:21

... m M phenylmethanesulfonyl fluoride. Cells were then disrupted by treatment with lysozyme (1 mgÆmL )1 )onice for 10 min followed by a single rapid cycle of freeze and thaw at 4 °C. The entire cell lysate ... at the beginning of the stationary phase the rate of synthesis of heat-shock proteins increases considerably, but only transiently [2 ,18 ]. Similarly, we obse...

Ngày tải lên: 22/02/2014, 07:20

... oxidation of the heme groups of the enzyme by various compounds was tested to identify the physiological electron acceptor of the enzyme. The enzyme was not oxidized by the heterodisulfide of coenzyme ... amounts of th e 16 -kDa c-type cytochrome. The Fig. 2. Genomic o rganization of the genes e ncoding the subunits of the Hme complex from A. fulgidus....

Ngày tải lên: 21/02/2014, 03:20

... Phosphatidylinositol synthesis and exchange of the inositol head are catalysed by the single phosphatidylinositol synthase 1 from Arabidopsis Anne-Marie Justin, Jean-Claude Kader and Sylvie ... host naturally devoid of phosphatidylinositol (PtdIns). In the context of the bacterial membrane and in addition to de novo synthesis, the plant enz...

Ngày tải lên: 22/02/2014, 04:20

... cleaved by the restriction enzymes Nde1 and Xho1 and inserted between the Nde1 and the Xho1 sites of the pET21b. The HCV NS5B-1b and the GBV-B NS5B were purified following the same procedure as for the ... efficiently replicates the 3 41 nt of the 3¢-end of the HCV minus-strand RNA in vitro and that deletion of the 3¢ 45 nt greatly impaired RNA synt...

Ngày tải lên: 20/02/2014, 01:20

... The phosphoryl and pyridoxyl moieties of the P-pyridoxyl group are shown in red and magenta, respectively. The fractional solvent exposed area of the P-pyridoxyl group is 0.39 and 0.082 for the ... concentrations. Substrate or ligand s 1 (ns) f 1 s 2 (ns) f 2 v 2 – 5 .15 0.34 1. 21 0.66 1. 1 ATP, Mn 2+ 5.27 0.37 1. 17 0.63 1. 06 PEP, Mg 2+ 5.52 0.47 1. 10 0.53...

Ngày tải lên: 21/02/2014, 01:21

... regulated by natural inhibitors belonging to the cystatin superfamily. These inhibitors are of three major types [1] . Those of type 1, cystatins A and B (also called stefins), are approximately 10 0 ... cells (1 · 10 6 mL )1 ) were seeded and cultured in the presence of 1 l M ATRA for 4 days or in the presence of 0 .13 l M TPA for 2 days. After incubation for...

Ngày tải lên: 21/02/2014, 01:21

... 265 .1 334.2 5 T6 630.3 520.3 4 T 6 315 .7 260.6 4 P7 727.4 419 .2 3P 7364.2 210 .1 3 F8 874.4 322.2 2 F 8 437.7 16 1.6 2 R9 10 30.6 17 5 .1 1R 9 515 .8 88 .1 1 (Val1,Thr3,Thr6)-bradykinin V1 10 0 .1 10 21. 5 ... 10 )6 M 1 · 10 )3 M ) exposures and the endothelial layer of the artery was removed by bubbling with oxygen for 10 s. Absence of the endothelial layer w...

Ngày tải lên: 21/02/2014, 15:20

... transduction by members of the TNF receptor and IL1/Toll-receptor family [1, 2]. The TRAF proteins are characterized by a C-terminal homology domain of about 200 amino acids, called the TRAF domain. The ... Henkler 2 , Ottmar Janssen 3 and Harald Wajant 1 1 Institute of Cell Biology and Immunology and 2 Institute of Industrial Genetics, University of Stuttga...

Ngày tải lên: 21/02/2014, 15:20