Tài liệu Báo cáo Y học: Evidence that a eukaryotic-type serine/threonine protein kinase from Mycobacterium tuberculosis regulates morphological changes associated with cell division docx

... 1085 PRIORITY PAPER Evidence that a eukaryotic-type serine/threonine protein kinase from Mycobacterium tuberculosis regulates morphological changes associated with cell division Rachna Chaba, Manoj Raje ... Mukhopadhyay, S., Kapatral, V., Xu, W. & Chakrabarty, A. M. (1999) Characterization of a Hank’s type serine/threonine kinase and a serine/th...

Ngày tải lên: 22/02/2014, 04:20

... procedures Materials Yeast nitrogen base without amino acids, phenylmethyl- sulfonyl fluoride, digitonin, Triton X-100, glass beads, acryl- amide, bis-acrylamide, N,N,N¢,N¢-tetramethylethylenediamine, ammonium ... because of the absence of the catalytic subunit ISP (Table 1). Figure 3A shows that a band of approximately 500 kDa was also found in this mutant strain when the mitochondrial m...

Ngày tải lên: 18/02/2014, 08:20

... d(5¢-AACAACGCAGCT GGGCTCTG GAACCAT), ECF -A1 41Q d(5¢-TCAACCTCTAAC CAG GCTACTCCGCTG) ECM-G77Q d(5¢-AACAACGCTGG C CAGCACGCTAACCAC) and ECM-Q14 6A d(5¢-TCT ACTGCTAAC GCGGATTCTCCGCTG) following the manufacturer’s ... performed with hydrogen peroxide and catalase to ensure there were no adverse effects on the SOD assay. Polyacrylamide gel electrophoresis (PAGE) Native 8% polyacrylamide gels (a...

Ngày tải lên: 21/02/2014, 01:21

... 5¢-ATGTTAAA GAAAGTATTAAGTTGTTG-TATTGCAGC-3¢ and 5¢-AAAGTTTGCTTGGAGCGTCAAGCC-ACTGTAAG CCG-3¢, cloned into the expression vector pBAD-TOPO, transformed into the E.coliTop10 strain and grown on LB agar ... triose-phosphate isomerase [24], a- amylase [27,28], and that of malate dehydrogenase [31] have been published. The psychrophilic enzymes characterized so far generally have higher catalyti...

Ngày tải lên: 21/02/2014, 01:21

... utative signal sequence. After treatment with tunicamycin, a glycosylation inhibitor, BmUGT1 migrated more rapidly, with an estima te d molecular mass o f 55 k Da, indicating that it was N-glycosylated. ... project. A wing disc cDNA library derived from fifth instar B. mori C108 larvae was kindly provided by Dr Kawasaki (University of Utsunomiya, Japan). A total of 1000 clones we...

Ngày tải lên: 22/02/2014, 04:20

... Tokumura, A. , Iimori, M., Nishioka, Y. , Kitahara, M., Sakashita, M. & Tanaka, S. (1994) Lysophosphatidic acids induce pro- liferation of cultured vascular smooth muscle cells from rat aorta. Am. ... cells Kentaro Kogure, Motoki Morita, Susumu Hama, Sawa Nakashima, Akira Tokumura and Kenji Fukuzawa 1 Faculty of Pharmaceutical Sciences, University of Tokushima, Japan The effect of a-...

Ngày tải lên: 22/02/2014, 04:20

... computational analysis. For many years, it was generally assumed that lipases are poorly active against soluble esters a nd become mark edly active when the solubility limit of the substrate is ... kinetic behaviors unambiguously classify Rv1399c as a nonlipolytic rather than a lipolytic hydrolase. Sequence alignment reveals that this enzyme b elongs to the a/ b hydrolase fold fami...

Ngày tải lên: 07/03/2014, 16:20

... those of extradiol dioxygenases available in the FASTA AND BLAST database programs at the DNA Data Bank of Japan. The gene encoding 4-amino-3-hydrox- ybenzoate 2,3-dioxygenase is currently being cloned; ... (Extract Ehlrich) was from Kyokuto Seiyaku Kogyo (Osaka, Japan) and 4-aminoresorcinol hydrochloride was from Aldrich (Milwaukee, Wis., USA). DE52 cellulose was from What- man (Madiso...

Ngày tải lên: 21/02/2014, 01:21

... & Tokuyasu, K. (1989) Analysis of unsaturated disaccharides from glycosami- noglycuronan by high-performance liquid chromatography. Anal Biochem. 177, 327–332. 37. Reese, C .A. & Mayne, R. ... action on platelet adhesion and activation by only two peptides from type II collagen (data not shown) and only by peptide a1 (I) CB3 from t ype I collagen, as already known from the li...

Ngày tải lên: 21/02/2014, 15:20

... de Salamanca, Edi®cio Departamental, Avda, Salamanca, Spain; 2 Centro Hispano-Luso de Investigaciones Agrarias, Universidad de Salamanca, Edi®ci o Departamental, Avda, Salamanca, Spain The Phycomyces ... Complementation analysis To characterize genetically the mutant strain A4 86, a complementation analysis was performed by making hetero- karyons between this mutant strain and representati...

Ngày tải lên: 22/02/2014, 04:20