Tài liệu Báo cáo Y học: Divergent members of a soybean (Glycine max L ) 4-coumarate:coenzyme A ligase gene family potx

Tài liệu Báo cáo Y học: Divergent members of a soybean (Glycine max L.) 4-coumarate:coenzyme A ligase gene family potx

... parentheses): Arabidopsis thaliana 4CL1 (U1867 5), A. thaliana 4CL2 (AF10608 6), A. thaliana 4CL3(AF10608 8), G. max 4CL1 (AF27926 7), G. max 4CL2(AF00225 9), G. max 4CL3 (AF00225 8), G. max 4CL4(X6995 5), ... 26 9) 1315 Divergent members of a soybean (Glycine max L. ) 4-coumarate:coenzyme A ligase gene family Primary structures, catalytic properties, and differential expressionChristian Lindermayr1, ... (198 9) Rapid induction of phenylalanine ammonia-lyase and chalcone synthase mRNAsduring fungus infection of soybean (Glycine max L. ) r oots orelicitor treatment of soybean cell cultures at the...

Tài liệu Báo cáo Y học: The structures of the lipooligosaccharide and capsule polysaccharide of Campylobacter jejuni genome sequenced strain NCTC 11168 pdf

... GalNAc-(1ﬁ 4)- Gal-(1ﬁ 3)- Gal+826 GalNAc-(1ﬁ 4)- Gal+3›2Neu5Ac872 GalNAc-(1ﬁ 4)- Gal-(1ﬁ 3)- Gal+3›2Neu5Ac1029 Gal-(1ﬁ 3)- GalNAc-(1ﬁ 4)- Gal+3›2Neu5Ac1120 GalNAc-(1ﬁ 4)- Gal-(1ﬁ 3)- Gal-(1ﬁ 3)- Hep+2›1Gal1233 ... Cj1427cF918 (5¢-AACTTTCATCATTTTAAACGCTCTT-3 ) and Cj1427cR10 (5¢-AAAGTTTTAATTACAGGTGGTGCAG-3 ). Deoxycholate-PAGE analysis and silver staining of polysaccharidesProteinase K-treated whole cells of C. ... phase variability of wlaN. The lack of terminal GalNAc in this strain has recently been shown byGilbert et al. to be due to the inactivation of the N-acetyl-galactosaminyltransferase, CgtA,...

Tài liệu Báo cáo Y học: Identiﬁcation of a set of genes involved in the biosynthesis of the aminonucleoside moiety of antibiotic A201A from Streptomyces capreolus pdf

... modes of action as antibacterial (puromycin), plant antifungal(blasticidin S, mildiomycin), antiviral (oxetanocin, Ara -A) ,antitumoral (oxanosine, neplanocin A) , herbicidal (poly-oxins), insecticidal ... thiostrepton was used at a ﬁnalconcentration of 10 lgÆmL )1 and 25 lgÆmL )1 in liquidand agar media, respectively, whereas hygromycin B wasadded to a ﬁnal concentration of 200 lgÆmL )1 .Transformation ... similarities with several products from the purcluster of S. alboniger [6]. They were accordingly namedataP3, ataP5, ataP4, ataP10 and ataP7. The two additionalones were named ata12 and ataPKS1...

Tài liệu Báo cáo Y học: Functional analysis of DM64, an antimyotoxic protein with immunoglobulin-like structure from Didelphis marsupialis serum pdf

... (M13F-cccagtcacgacgttgtaaaacg- and M13R-agcggataacaatttcacacagg) were fromLife Technologies, Inc. All other chemicals were of analy-tical grade or higher quality.Animals, venoms, and toxinsD. marsupialis ... 26 9) 6053(43 kDa), carbonic anhydrase (30 kDa), soybean trypsininhibitor (20.1 kDa) and a- lactalbumin (14.4 kDa).Molecular massDM64 molecular mass was determined by MALDI-TOFMS on a Voyager ... (43 kDa),chymotrypsinogen (25 kDa) and ribonuclease (13.7 kDa).Di-BSA (134 kDa) present in the BSA standard was alsoused as marker.Chemical deglycosylationDM64 was chemically deglycosylated...

Tài liệu Báo cáo Y học: Dynamic mechanism of nick recognition by DNA ligase ppt

... (200 0) Crystallization and preliminary X-ray crystallographic analysis of NAD+-dependent DNA ligase from Thermus ﬁliformis. ActaCrystallogr. D Biol Crystallogr. 56, 357–358.21. Lee, J .Y. , Chang, ... Two of them, Lys238 and Lys240 of T7 DNA ligase (Lys188 and to a lesser extent Lys186 of Chlorellavirus DNA ligase) were shown to be essential for thetransadenylation and nick sealing activities ... this transition in the DNA ligase catalysis?DYNAMIC MECHANISM OF NICKRECOGNITION BY DNA LIGASE –AHYPOTHESISAccording to Doherty et al. [25,34], the adenylylated DNA ligase binds dsDNA forming...

Tài liệu Báo cáo Y học: The effects of ring-size analogs of the antimicrobial peptide gramicidin S on phospholipid bilayer model membranes and on the growth of Acholeplasma laidlawii B ppt

... ring-sizeanalogs of GS on the growth of A. laidlawii B, a cell wall-lessGram positive bacteria (Mollicute). The absence of a lipopolysaccharide-containing cell wall or outer membraneor a lipopeptidoglycan ... effect of these analogs on thepermeability of LUVs composed of 1-palmitoyl-2-oleoyl-glycerophosphocholine (PamOleGro-PCho), 1-palmitoyl-2-oleoylglycerophosphoethanolamine (PamOleGro-PEtn)and ... PamOleGro-PCho, 1-palmitoyl-2-oleoyl-glycerophosphocholine; PamOleGro-PEtn, 1-palmitoyl-2-oleoyl-glycerophospholamine; PamOleGro-PGro, 1-palmitoyl-2-oleoyl-glycerophosphoglycerol.*Present address: Department...

Tài liệu Báo cáo Y học: Novel bradykinins and their precursor cDNAs from European yellow-bellied toad (Bombina variegata) skin potx

... (braykinyl-isoleucyl-tyrosine-0-sulphate)and bradykinyl-isoleucyl-tyrosine. Br.J.Pharmacol.27, 479–485.16. Yasuhara, T., Hira, M., Nakajima, T., Yanaihara, N. &Yanaihara, C. (197 3) Active ... wasachieved by acid hydrolysis of a known gravimetric quantity of lyophilisate followed by amino acid analysis using anApplied Biosystems PTH-amino acid analyser.cDNA cloningDorsal skin was ... (Val1,Thr 6)- bradykinin and (Val1,Thr 6)- bradykinyl-VAPAS inR. nigromaculata [13,14]. Additional structural variantssuch as phyllokinin (bradykinyl-IYsulfated) have beenfound in the skins of several...

Tài liệu Báo cáo Y học: Novel complexes of mammalian translation elongation factor eEF1AÆGDP with uncharged tRNA and aminoacyl-tRNA synthetase potx

... elskaya@biosensor.kiev.uaAbbreviations: ARS, aminoacyl-tRNA synthetase;eEF 1A, eukaryotic translation elongation factor 1A (formerly EF- 1a) ;EF 1A, prokaryotic translation elongation factor 1A (formerlyEF-Tu); FITC, ... transfer of tRNA/aminoacyl-tRNA duringmammalian translation elongation cycleRecently, the crystal structure of the [eEF 1A eEF1Ba]complex became available revealing a possibility of competition ... tRNA/aminoacyl-tRNA channeling in thetranslation elongation cycle. d, amino acid; small and large triangles,tRNA and eEF1Ba, respectively.Fig. 5. Biosensor assay of the quaternary [eEF 1A GDPÆtRNAPheÆPheRS]...

Tài liệu Báo cáo Y học: Molecular modeling of the dimeric structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain docx

... structure;heparin binding; substrate recognition; catalytic activity.Lipoprotein lipase (LPL) belongs to a mammalian lipase family that includes pancreatic lipase (PL), hepatic lipase(HL), gastric lipase, ... b-D-galactosidase, and the other wascoupled with insoluble bacterial cell walls. LPL concentra-tion was measured as the catalytic activity with 4-methyl-umbelliferyl b-D-galactoside as a substrate. ... (University of Utah, Salt LakeCity, UT, USA), or anti-(His-tag) polyclonal Ig (Medicaland Biological Laboratories Co., Nagoya, Japan) was usedto detect LPL or His-tagged proteins. Bound antibody wasreacted...

Tài liệu Báo cáo Y học: Identiﬁcation of syntaxin-1A sites of phosphorylation by casein kinase I and casein kinase II ppt

... inaseswidely expressed in a range of eukaryotes including yeast,mammals and plants. They have been shown to play a role indiverse physiological events including membrane traﬃcking.CKIa is associated ... C-terminal to Arg or Lys(i.e. K/R-X-Sp/Tpbonds [30 ]). Release of 32P at each cycle of Edman degradation on covalently coupled peptides afterphosphorylation of syntaxin- 1A (1–26 5) by CKII andpuriﬁcation ... (Fig. 6). ThisFig. 4. CKIa phosphorylates in vitro syntaxin 1 A on Thr21. Release of 32P at each cycle of automatic Edman degradation after phosphory-lation of syntaxin- 1A by C KIa and puriﬁcation...

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