... parentheses): Arabidopsis thaliana 4CL1 (U1867 5), A. thaliana 4CL2 (AF10608 6), A. thaliana 4CL3 (AF10608 8), G. max 4CL1 (AF27926 7), G. max 4CL2 (AF00225 9), G. max 4CL3 (AF00225 8), G. max 4CL4 (X6995 5), ... 26 9) 1315 Divergent members of a soybean ( Glycine max L. ) 4-coumarate:coenzyme A ligase gene family Primary structures, catal...
Ngày tải lên: 22/02/2014, 04:20
... GalNAc-(1fi 4)- Gal-(1fi 3)- Gal + 826 GalNAc-(1fi 4)- Gal + 3 › 2 Neu5Ac 872 GalNAc-(1fi 4)- Gal-(1fi 3)- Gal + 3 › 2 Neu5Ac 1029 Gal-(1fi 3)- GalNAc-(1fi 4)- Gal + 3 › 2 Neu5Ac 1120 GalNAc-(1fi 4)- Gal-(1fi 3)- Gal-(1fi 3)- Hep + 2 › 1 Gal 1233 ... Cj1427cF918 (5¢-AACTTTCATCATTTTAAAC GCTCTT-3 ) and Cj1427cR10 (5¢-AAAGTTTTAATTAC AGGTGGTGCAG-3 ). Deoxycholate-PAGE analysis and...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: Identification of a set of genes involved in the biosynthesis of the aminonucleoside moiety of antibiotic A201A from Streptomyces capreolus pdf
... modes of action as antibacterial (puromycin), plant antifungal (blasticidin S, mildiomycin), antiviral (oxetanocin, Ara -A) , antitumoral (oxanosine, neplanocin A) , herbicidal (poly- oxins), insecticidal ... thiostrepton was used at a final concentration of 10 lgÆmL )1 and 25 lgÆmL )1 in liquid and agar media, respectively, whereas hygromycin B was added to a final concentrati...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: Functional analysis of DM64, an antimyotoxic protein with immunoglobulin-like structure from Didelphis marsupialis serum pdf
... (M13F-cccagtcacgacgttg taaaacg- and M13R-agcggataacaatttcacacagg) were from Life Technologies, Inc. All other chemicals were of analy- tical grade or higher quality. Animals, venoms, and toxins D. marsupialis ... 26 9) 6053 (43 kDa), carbonic anhydrase (30 kDa), soybean trypsin inhibitor (20.1 kDa) and a- lactalbumin (14.4 kDa). Molecular mass DM64 molecular mass was determined by MAL...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: Dynamic mechanism of nick recognition by DNA ligase ppt
... (200 0) Crystallization and preliminary X-ray crystallographic analysis of NAD + -dependent DNA ligase from Thermus filiformis. Acta Crystallogr. D Biol Crystallogr. 56, 357–358. 21. Lee, J .Y. , Chang, ... Two of them, Lys238 and Lys240 of T7 DNA ligase (Lys188 and to a lesser extent Lys186 of Chlorella virus DNA ligase) were shown to be essential for the transadenylation an...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: The effects of ring-size analogs of the antimicrobial peptide gramicidin S on phospholipid bilayer model membranes and on the growth of Acholeplasma laidlawii B ppt
... ring-size analogs of GS on the growth of A. laidlawii B, a cell wall-less Gram positive bacteria (Mollicute). The absence of a lipopolysaccharide-containing cell wall or outer membrane or a lipopeptidoglycan ... effect of these analogs on the permeability of LUVs composed of 1-palmitoyl-2-oleoyl- glycerophosphocholine (PamOleGro-PCho), 1-palmitoyl- 2-oleoylglycerophosphoeth...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: Novel bradykinins and their precursor cDNAs from European yellow-bellied toad (Bombina variegata) skin potx
... (braykinyl-isoleucyl-tyrosine-0-sulphate) and bradykinyl-isoleucyl-tyrosine. Br.J.Pharmacol.27, 479–485. 16. Yasuhara, T., Hira, M., Nakajima, T., Yanaihara, N. & Yanaihara, C. (197 3) Active ... was achieved by acid hydrolysis of a known gravimetric quantity of lyophilisate followed by amino acid analysis using an Applied Biosystems PTH-amino acid analyser. cDNA cloning Dorsal s...
Ngày tải lên: 21/02/2014, 15:20
Tài liệu Báo cáo Y học: Novel complexes of mammalian translation elongation factor eEF1AÆGDP with uncharged tRNA and aminoacyl-tRNA synthetase potx
... elskaya@biosensor.kiev.ua Abbreviations: ARS, aminoacyl-tRNA synthetase; eEF 1A, eukaryotic translation elongation factor 1A (formerly EF- 1a) ; EF 1A, prokaryotic translation elongation factor 1A (formerly EF-Tu); FITC, ... transfer of tRNA/aminoacyl-tRNA during mammalian translation elongation cycle Recently, the crystal structure of the [eEF 1A eEF1Ba] complex became available reveal...
Ngày tải lên: 21/02/2014, 15:20
Tài liệu Báo cáo Y học: Molecular modeling of the dimeric structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain docx
... structure; heparin binding; substrate recognition; catalytic activity. Lipoprotein lipase (LPL) belongs to a mammalian lipase family that includes pancreatic lipase (PL), hepatic lipase (HL), gastric lipase, ... b- D -galactosidase, and the other was coupled with insoluble bacterial cell walls. LPL concentra- tion was measured as the catalytic activity with 4-methyl- umbelliferyl b- D -ga...
Ngày tải lên: 21/02/2014, 15:20
Tài liệu Báo cáo Y học: Identification of syntaxin-1A sites of phosphorylation by casein kinase I and casein kinase II ppt
... inases widely expressed in a range of eukaryotes including yeast, mammals and plants. They have been shown to play a role in diverse physiological events including membrane trafficking. CKIa is associated ... C-terminal to Arg or Lys (i.e. K/R-X-S p /T p bonds [30 ]). Release of 32 P at each cycle of Edman degradation on covalently coupled peptides after phosphorylation of syntaxi...
Ngày tải lên: 22/02/2014, 04:20