... suppliedwith the enzyme, and contained Aba, Abb and Abcat40 nm each, and the start and stop primers Abstart and Abstop at 600 nm each, and 200 lm each of dATP, dCTP,dGTP and dTTP. The product ... of the purification, and the require-ment for HPLC increases the length and complexity ofthe purification procedure. Moreover, as with thestudies by Lee et al. [21] and Subramanian and Shree[42], ... substitutions, and we have produced some suchpeptides in this study. Importantly, the protocoldescribed for expression and purification of Ab(M1–40) and Ab(M1–42) is inexpensive, relatively rapid and only...