... lM) and ALLN (20 lM, 0.2 lM); (B) caspase inhibitors, Z-VAD-FMK (50 lM for pan caspase), Z-YVAD-FMK (50 lM for caspase-1 ⁄ 4),Z-VDVAD-FMK (50 lM for caspase-2) and Z-DEVD-FMK (50 lM for caspase-3 ... lgofEmGFP-pcDNA3.1 and 27 lL of FuGENE6, and incubated at37 °C for 48 h. After washing twice with ice-cold wash buf-fer, the cells were incubated with 1 mL of extraction bufferIat4°C for 10 min, and the ... 4887Geriatrics and Gerontology (NCGG); a Grant-in-Aid for Research on Intractable Diseases from the Ministryof Health, Labor and Welfare, Japan; and OkinakaMemorial Institute for Medical Research.References1...