... Identification of 20,23-dihydroxyvitamin D3. (A) Expansion of proton–proton COSY correlations for 3-CH and 23-CH; (B) expan-sion of proton–proton TOCSY correlations for 3-CH and 23-CH; (C)expansion of ... above.Preparation of minor products to test assubstrates for P450sccMinor metabolites of vitamin D3 were purified from50 mL incubations of 100 lm vitamin D3 with 2 lm P450,as described above for the ... School of Biomolecular, Biomedical and Chemical Sciences, The University of Western Australia, Crawley, Australia2 Department of Pharmaceutical Sciences, College of Pharmacy, University of Tennessee...