... ofVrp1p and Las17p in yeast and WIP and WASP-fam-ily proteins in mammalian cells are direct and resultin constitutive and apparently stable complexes[28,29,31,32]. In yeast, Vrp1p and Las17p ... functionsof Vrp1p in endocytosis, cytokinesis, and growth atelevated temperature, on the one hand, and in corticalactin-patch polarization, on the other hand, are at leastpartially distinct [23]. ... interaction with actin and that GST-C-Vrp1p465)533binds endogenous actin in yeast lysates and directly binds purified yeast and rabbit skeletalmuscle G-actin in vitro (Fig. 4B–D and data notshown)....