... iscytosolic and its activity is absent in resting cells, appears in late G1,increases in S phase coinciding with the increase in DNA synthesis, anddisappears during mitosis. The fluctuation ... and molecular mass analyses. Theactivity at saturating conditions was similar before andafter dilution, incubation and storage.Native molecular sizeThe apparent molecular size was determined ... non-radioactive nucleosides and molecularmass markers were purchased from Sigma-Aldrich (Copen-hagen, Denmark). Materials for cloning, PCR, DNA seq-uencing and assays were standard commercially...