Tài liệu Báo cáo Y học: Steady-state kinetics of the glutaminase reaction of CTP synthase from Lactococcus lactis The role of the allosteric activator GTP in coupling between glutamine hydrolysis and CTP synthesis potx

... mass of % 97 kDa. The fu sion protein was purified from the s oluble f raction b y affinity chromatography using amylose resin. In vitro kinase assays showed that the autophosphorylating ability o ... division. Finally, our study in a heterologous setting has shown the involvement of PknA in cell shape regulation; it is the first report describing the functionality of...

Ngày tải lên: 22/02/2014, 04:20

... proteins they probably form part of the copper binding sites in this protein. Although there are four histidines in the C-terminal, another histidine is present at position 18, and given the folded ... protein with glycine alone was performed and no binding was observed. In our hands, as others [54], the most reproducible data was obtained from injections of the meta...

Ngày tải lên: 19/02/2014, 06:20

... Steady-state kinetics of the glutaminase reaction of CTP synthase from Lactococcus lactis The role of the allosteric activator GTP in coupling between glutamine hydrolysis and CTP synthesis Martin ... lactis CTP synthase is suggested. Here the binding of GTP to the allosteric site promotes coordination of the phosphoryl...

Ngày tải lên: 21/02/2014, 15:20

... allowing the user to select from a list of reactions. The user can then specify the direction of the reaction, and which substrate concentration was varied during the assays. Enzyme kinetics: from ... without any indi- cation of the enzyme concentration contained within the term. To prevent this, the kineticswizard cap- tures the enzyme concentration used in...

Ngày tải lên: 18/02/2014, 04:20

... high binding affinity of PEP in the presence of Mn 2+ [28]. The high affinity of PEP in the presence of Mn 2+ suggests a specific interaction between these two ligands in the enzyme active site. In the ... characteristics of the native protein, allowing the determination of several pro- tein–ligand dissociation constants, as well as the role of Mg 2+ an...

Ngày tải lên: 21/02/2014, 01:21

... that dephosphorylation and/ or catalytic turnover of EII Glc , rather than binding of Glc, enhanced the reactivity of Cys421. As Cys421 is the only invariant cysteine in homologous transporters and also the ... were recently introduced for the preparation of C-6 aldehyde derivatives of Glc [15]. The key step is the use of Collins reagent for the selective oxidat...

Ngày tải lên: 21/02/2014, 01:21

... [6,7]. The methylation pattern is created by de novo methylation and demethyla- tion of the DNA, and maintained during mitosis by maintenance methylation [reviews 8,9]. De novo methyla- tion of DNA ... accurate maintenance methylation in these regions. On the other hand, Dnmt3a and Dnmt3b are not capable of efficient de novo methylation of DNA in the absence of Dnmt...

Ngày tải lên: 21/02/2014, 01:21

... Expression of lacA is also detected in the presence of these compounds, but in the presence of D -xylose and xylan as well. This can be explained by the structure of the polysaccharides. Xylan contains ... activity of GALA could be detected against any of the transgalactooligosaccharides listed in Materials and methods. Hydrolysis of arabinogalactans The...

Ngày tải lên: 21/02/2014, 01:21

... was the extremely high thermostability of Mn[G77Q] and the very low thermostability of Fe[A141Q] (Fig. 5). Each of these mutants has two glutamine residues in their active sites; one from the ... distinguish between FeSOD and MnSOD [7]. The position of the glutamine residue, which is structurally equivalent in the enzymes, is contributed by the N domain...

Ngày tải lên: 21/02/2014, 01:21

... characterization of specific enzymes that are involved in the biosynthesis of these structures and may lead to the discovery of potential therapeutic targets. In addition, the correlation of carbohydrate ... we are currently investigating this possibility. Alternatively, this enzyme may be involved in the epimerization reaction necessary for the biosynthesis of t...

Ngày tải lên: 21/02/2014, 01:21