Tài liệu Báo cáo Y học: Steady-state kinetics of the glutaminase reaction of CTP synthase from Lactococcus lactis The role of the allosteric activator GTP in coupling between glutamine hydrolysis and CTP synthesis potx
Tài liệu Báo cáo Y học: Evidence that a eukaryotic-type serine/threonine protein kinase from Mycobacterium tuberculosis regulates morphological changes associated with cell division docx
... mass of % 97 kDa. The fu sion protein was purified from the s oluble f raction b y affinity chromatography using amylose resin. In vitro kinaseassays showed that the autophosphorylating ability o ... division.Finally, our study in a heterologous setting has shown the involvement of PknA in cell shape regulation; it is the firstreport describing the functionality of any eukaryotic-typeSer/Thr kinase ... ability of the fusion proteinwas monitored b y incubating it with [c-32P]ATP in t hepresence of Mn2+, f ollowed by separation of reaction products by SDS/PAGE. Finally, the labelled protein...
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Tài liệu Báo cáo khoa học: High affinity copper binding by stefin B (cystatin B) and its role in the inhibition of amyloid fibrillation docx
... proteins they probably formpart of the copper binding sites in this protein.Although there are four histidines in the C-terminal,another histidine is present at position 18, and given the folded ... protein withglycine alone was performed and no binding was observed. In our hands, as others [54], the most reproducible datawas obtained from injections of the metal into a solution of the ... (cystatin B) is a high affinitycopper binding protein, and (b) that amyloid fibrilformation of this protein is diminished in presence of Cu2+ions. Another interesting finding is thatcopper binding...
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Tài liệu Báo cáo Y học: Steady-state kinetics of the glutaminase reaction of CTP synthase from Lactococcus lactis The role of the allosteric activator GTP in coupling between glutamine hydrolysis and CTP synthesis potx
... Steady-state kinetics of the glutaminase reaction of CTP synthase from Lactococcus lactis The role of the allosteric activator GTP in coupling between glutamine hydrolysis and CTP synthesis Martin ... lactis CTP synthase is suggested. Here the binding of GTP to the allosteric site promotes coordination of the phosphorylation of UTP and hydrolysis of glutamine foroptimal efficiency in CTP synthesis ... Lactococcus lactis. CTP synthase (EC 6.4.3.2) catalyzes the synthesis of CTP from UTP by amination of the pyrimidine ring at the 4-position. The enzyme has three functionally distinct sites; the glutaminase...
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Tài liệu Báo cáo khoa học: Enzyme kinetics informatics: from instrument to browser pdf
... allowing the user toselect from a list of reactions. The user canthen specify the direction of the reaction, and which substrate concentration wasvaried during the assays.Enzyme kinetics: from ... without any indi-cation of the enzyme concentration contained within the term. To prevent this, the kineticswizard cap-tures the enzyme concentration used in the assay,allowing the kinetic parameter ... deriving kinetic data from the laboratory instrument and make it accessible in the web. The task of managing enzyme kinetics datainvolves four steps: data capture, analysis, submission and querying...
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Tài liệu Báo cáo Y học: Ligand interactions and protein conformational changes of phosphopyridoxyl-labeled Escherichia coli phosphoenol pyruvate carboxykinase determined by fluorescence spectroscopy pdf
... highbinding affinity of PEP in the presence of Mn2+[28]. The high affinity of PEP in the presence of Mn2+suggests aspecific interaction between these two ligands in the enzymeactive site. In the ... characteristics of the native protein, allowing the determination of several pro-tein–ligand dissociation constants, as well as the role of Mg2+ and Mn2+ in substrate binding. The binding affinity of PEP ... the binding of several ligands and on the role of Mn2+ and Mg2+on theirbinding. The comparison between acrylamide quenchingstudies and modeled structures of free and substrate boundP-pyridoxyl–E....
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Tài liệu Báo cáo Y học: The glucose-specific carrier of the Escherichia coli phosphotransferase system Synthesis of selective inhibitors and inactivation studies pptx
... thatdephosphorylation and/ or catalytic turnover of EIIGlc,rather than binding of Glc, enhanced the reactivity of Cys421. As Cys421 is the only invariant cysteine in homologous transporters and also the ... wererecently introduced for the preparation of C-6 aldehydederivatives of Glc [15]. The key step is the use of Collinsreagent for the selective oxidation of the primary trimethyl-silyl ether of the ... G. (1986) The oxidation of primary trimethylsilyl ethers toaldehydes: a selective conversion of a primary hydroxy group intoan aldehyde group in the presence of a secondary hydroxy group.J....
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Tài liệu Báo cáo Y học: Dnmt3a and Dnmt1 functionally cooperate during de novo methylation of DNA pdf
... [6,7]. The methylationpattern is created by de novo methylation and demethyla-tion of the DNA, and maintained during mitosis bymaintenance methylation [reviews 8,9]. De novo methyla-tion of DNA ... accurate maintenance methylation in these regions. On the other hand, Dnmt3a and Dnmt3b are not capable of efficient de novo methylation of DNA in the absence of Dnmt1. Whereas the maintenance methylation ... methylation of hemimethylated DNA in vitro [17,18]. However, Dnmt1also shows capabilities for de novo methylation of DNA[15]. Interestingly, the de novo activity of Dnmt1 isstimulated by binding...
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Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf
... Expression of lacA is also detected in the presence of these compounds, but in the presence of D-xylose and xylan as well. This can be explained by the structure of the polysaccharides. Xylan contains ... activity of GALA could be detected against any of the transgalactooligosaccharides listed in Materials and methods. Hydrolysis of arabinogalactans The sugar composition of the arabinogalactans from potato, ... made by introducingan NsiI site at the translation start point of galA and by thencloning a fragment, starting at this NsiI site and containing the galA gene and approximately 700 bp 3¢-flanking...
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Tài liệu Báo cáo Y học: Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue pdf
... was the extremely high thermostability of Mn[G77Q] and the very low thermostability of Fe[A141Q](Fig. 5). Each of these mutants has two glutamine residues in their active sites; one from the ... distinguish between FeSOD and MnSOD [7]. The position of the glutamine residue, which isstructurally equivalent in the enzymes, is contributed by the N domain of FeSODs (Q69) and the C domain of MnSODs(Q146). ... wild-type enzymes, the metal sites of the purified proteins are either completely(FeSOD, 1.17) or nearly fully occupied by the metal ion from which the SOD derives, and very little of the contrarymetal...
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Tài liệu Báo cáo Y học: The structures of the lipooligosaccharide and capsule polysaccharide of Campylobacter jejuni genome sequenced strain NCTC 11168 pdf
... characterization of specific enzymes thatare involved in the biosynthesis of these structures and maylead to the discovery of potential therapeutic targets. In addition, the correlation of carbohydrate ... we are currently investigating thispossibility. Alternatively, this enzyme may be involved in the epimerization reaction necessary for the biosynthesis of the capsular heptopyranose as alditol ... cell-surface molecules of the host and having the ability to vary these structures plays an important role in pathogenesis and survival within the environment. In the capsular polysaccharide of NCTC 11168,...
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