Tài liệu Báo cáo Y học: NMR-based determination of the binding epitope and conformational analysis of MUC-1 glycopeptides and peptides bound to the breast cancer-selective monoclonal antibody SM3 pptx
Tài liệu Báo cáo Y học: A novel, inducible, citral lyase purified from spores of Penicillium digitatum docx
... as the standard. RESULTS Induction of citral lyase Initially, the reproducibility of the results was hindered by variations in the citral lyase activity of the P. digitatum spores. Remarkably, ... hydratase and aldolase activity in a single enzyme. No other enzyme has been reported to catalyse the conversion of citral into methylheptenone and acetaldehyde, or a simil...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: Systematic search for zinc-binding proteins in Escherichia coli potx
... the Zn(II) -binding activity and to estimate the Zn(II) -binding affinity for the newly identified Zn(II)- binding proteins, we next cloned the genes encoding some of the newly identified Zn(II) -binding ... influence of the His 6 tag on the Zn(II) -binding activity was then tested. The His 6 tag added to the test proteins was found to show a low level of th...
Ngày tải lên: 22/02/2014, 04:20
Tài liệu Báo cáo Y học: NMR-based determination of the binding epitope and conformational analysis of MUC-1 glycopeptides and peptides bound to the breast cancer-selective monoclonal antibody SM3 pptx
... NMR of MUC-1 glycopeptides (Eur. J. Biochem. 269) 1455 NMR-based determination of the binding epitope and conformational analysis of MUC-1 glycopeptides and peptides bound to the breast cancer-selective ... enhances binding affinity of glycopeptides to the antibody [18]. Conventional pepscan analysis however, does not allow easy analys...
Ngày tải lên: 21/02/2014, 15:20
Tài liệu Báo cáo khoa học: Structure determination and biochemical studies on Bacillus stearothermophilus E53Q serine hydroxymethyltransferase and its complexes provide insights on function and enzyme memory doc
... interaction with the phenolate oxygen of Y5 1, N of G257 and NE2 of Q53 of the symmetry related subunit. Another oxygen atom is held by hydrogen bonding to NE2 of H122 and phenolate oxygen of Y5 1 of the symmetry related ... breakdown of the two- fold symmetry and hence to the monoclinic crystal form [12]. Attempts to crystallize E53QbsSHMT in the pre...
Ngày tải lên: 18/02/2014, 16:20
Tài liệu Báo cáo Y học: Ligand interactions and protein conformational changes of phosphopyridoxyl-labeled Escherichia coli phosphoenol pyruvate carboxykinase determined by fluorescence spectroscopy pdf
... The phosphoryl and pyridoxyl moieties of the P-pyridoxyl group are shown in red and magenta, respectively. The fractional solvent exposed area of the P-pyridoxyl group is 0.39 and 0.082 for the ... 2002 conformational changes caused by the ligand are easily sensed by the P-pyridoxyl chromophore. Figure 4 shows the modeled structure of the P-pyridoxyl-PEP carbox...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: The glucose-specific carrier of the Escherichia coli phosphotransferase system Synthesis of selective inhibitors and inactivation studies pptx
... G. (1986) The oxidation of primary trimethylsilyl ethers to aldehydes: a selective conversion of a primary hydroxy group into an aldehyde group in the presence of a secondary hydroxy group. J. ... were recently introduced for the preparation of C-6 aldehyde derivatives of Glc [15]. The key step is the use of Collins reagent for the selective oxidation of the prim...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: Dnmt3a and Dnmt1 functionally cooperate during de novo methylation of DNA pdf
... novo methylation of DNA [15]. Interestingly, the de novo activity of Dnmt1 is stimulated by binding of methylated DNA to an allosteric site located in the N-terminal domain of the enzyme [18,19]. ... development and even in adult cells to silence acquired proviral DNA or to alter the developmental program of the cell. Aberrant de novo methylation may lead to hy...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf
... 2 h and 20 h to determine the substrate specificity of the enzyme. The degradation of the polymer and subsequent oligosaccharide formation was analyzed by HPAEC and MALDI-TOF MS. HPAEC analysis ... potato resulting in a 30% reduction of the galactosyl content of the pectin fraction of the cell walls [17]. In this paper we describe the cloning, characterizati...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue pdf
... governed by one or even two residues, but is most likely accomplished by the concerted effects of a combination of key residues. Further analysis of these and other mutant SODs is currently underway. ACKNOWLEDGEMENTS We ... conclusive result. Effect of hydrogen peroxide on enzyme activity Hydrogen peroxide, the product of the SOD reaction, is an inhibitor of SODs and a...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: The structures of the lipooligosaccharide and capsule polysaccharide of Campylobacter jejuni genome sequenced strain NCTC 11168 pdf
... this possibility. Alternatively, this enzyme may be involved in the epimerization reaction necessary for the biosynthesis of the capsular heptopyranose as alditol acetate analysis (results not shown) and ... characterization of specific enzymes that are involved in the biosynthesis of these structures and may lead to the discovery of potential therapeutic targets. In...
Ngày tải lên: 21/02/2014, 01:21