Tài liệu Báo cáo Y học: Interaction of decorin with CNBr peptides from collagens I and II Evidence for multiple binding sites and essential lysyl residues in collagen pot

... peptides (Eur. J. Biochem. 269) 1437 Interaction of decorin with CNBr peptides from collagens I and II Evidence for multiple binding sites and essential lysyl residues in collagen Ruggero Tenni 1, *, ... the in uence on d ecorin binding of chemical modi- fication of Lys and Hyl side chains of collagens and peptides. Derivatizations...

Ngày tải lên: 21/02/2014, 15:20

... Ab1–42 peptides were solubilized in distilled water by trituration and sonication at 42 kHz for 5 min. In some experiments, peptides were incubated for 5 days at 37 °C in distilled water to induce ... to elicit a dose-dependent increase in toxicity in VSMC. In the presence of Ab peptides, this toxicity is exacerbated. This effect of homocysteine and Ab hasalsobeen...

Ngày tải lên: 22/02/2014, 07:20

... observed in the reaction with Cyt f, i. e. neutralizing acidic residues speeded the reaction up, and neutralizing or inverting the charge on basic residues slowed it down (Fig. 3 in [1], Fig. 2 in this ... wild-type P. laminosum plastocyanin reacting with wild-type P. laminosum photosystem I and wild-type spinach plastocyanin reacting with wild-type spinach photosystem...

Ngày tải lên: 21/02/2014, 01:21

... responsible for the binding of unfolded hydrophobic peptides and a N-terminal domain, which binds ATP. This protein can bind and release peptides (in the Ct domain) in a cycle driven by nucleotide hydrolysis and ... recently, methods were devised for predicting residues involved in protein interaction s ites in the absence of any structural reports. By analysing...

Ngày tải lên: 21/02/2014, 15:20

... by a very hydrophilic environment of W residues, exposed hydrophobic sites interacting with ANS and increased susceptibility to proteolysis. Exposure of hydrophobic sites increases the probability ... polymerization. In addition, this heating regime resembles that occurring in vivo under certain physiological conditions. In preliminary experiments we have shown that the inc...

Ngày tải lên: 20/02/2014, 23:20

... azide is contained within a pocket formed by no less than six residues all within 4 A ˚ (azide N1 comes within 2.1 A ˚ of the metal ion). Azide binding residues include the three His metal ligands ... Slower-migrating bands also visualized by SOD activity staining may have been derived from further interactions of the fusion protein GST domains (Fig. 2B). Although not visualized...

Ngày tải lên: 21/02/2014, 01:21

... study of linoleic acid radicals during the initiation of lipid peroxidation in Fenton’s reagent. Biophysics (Russ.) 29, 766–769. 84. Graceffa, P. (1988) Spin trapping the cysteine thiyl radical with ... efficiently interact with the E1 catalytic intermediate, as the lipoyl-bearing domain of E2 is designed for this interaction (reaction 2). In this case, 1e – oxidation of the...

Ngày tải lên: 21/02/2014, 01:21

... 0.20 Hydroxyethyl disulfide 0.11 Table 2. Inhibitor sensitivities of recombinant SmGSTO. Inhibitor sensitivities are presented as I 50 and were calculated in standard reac- tion conditions. Values in ... additional functions were attributed to GSTs including the transport of hydrophobic ligands, binding to bilirubin and carcinogens [3,4], the isomerization of maleylacetoaceta...

Ngày tải lên: 21/02/2014, 01:21

... (TEMPO) as mediators, since they greatly differ both in their mechan- ism of action and in their specificity. In fact, HPI is representative of a mediator that, after having been oxidized by laccase, ... obtained in the presence of an organic cosolvent, and also in the presence of two mediating species, i. e. N-hydroxyphthalimide (HPI) and 2,2,6,6-tetramethyl- piperidi...

Ngày tải lên: 21/02/2014, 01:21

... University of Iceland; and 2 Department of Biochemistry, Science Institute, University of Iceland, Reykjavik, Iceland The gene encoding a subtilisin-like serine proteinase in the psychrotrophic ... dithiothreitol under the same conditions as VPR wt both enzymes displayed identical inactivation behaviour, indicating that the same disulfide(s) are involved in maintaining the integrit...

Ngày tải lên: 21/02/2014, 01:21